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biorelevant FeSSIF-V2现货

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在模拟胃肠道液体的溶出介质中测试难溶性化合物有多大帮助，biorelevant开发了一种*的产品，可以立即产生这些液体（初称为“ SIF粉末”）。事实证明如此成功，我们决定集中精力为客户提供这些生物相关介质。

上海金畔biorelevant FeSSIF-V2现货

  

 

FeSSIF-V2

5个推荐

  • 制作第二版FeSSIF（进食状态模拟肠液）
  • 包含生理表面活性成分

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选择包装
0.5L大小（V2FES01）
5L大小（V2FES02）
50L大小（V2FES03）
4 X 0.5L尺寸（V2FES04）
4 X 5L尺寸（V2FES05）

 

 

 

 

pUB6/V5 His C说明书

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 pUB6/V5 His C

型号 载体名称 出品公司 载体用途
VPI0024 pUB6/V5 His C Invitrogen 哺乳动物表达载体

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Blasticidin
Promoter：UbC
Vector Type：pUB6
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：V5 Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

pUB6/V5-His A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells.

The vectors contain the following elements:

  1. Human ubiquitin C promoter （hUbC） for high-level expression across a broad range of species and cell types （Schorpp et al., 1996; Wulff et al., 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal peptide encoding the V5 epitope and a polyhistidine （6×His） metal-binding tag.
  3. Blasticidin resistance gene （bsd） for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g. COS-1, COS-7）.

The control plasmid, pUB6/V5-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: 
 

pUB6/V5 His B说明书

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 pUB6/V5 His B

型号 载体名称 出品公司 载体用途
VPI0023 pUB6/V5 His B Invitrogen 哺乳动物表达载体

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Blasticidin
Promoter：UbC
Vector Type：pUB6
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：V5 Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

pUB6/V5-His A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells.

The vectors contain the following elements:

  1. Human ubiquitin C promoter （hUbC） for high-level expression across a broad range of species and cell types （Schorpp et al., 1996; Wulff et al., 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal peptide encoding the V5 epitope and a polyhistidine （6×His） metal-binding tag.
  3. Blasticidin resistance gene （bsd） for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g. COS-1, COS-7）.

The control plasmid, pUB6/V5-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: 
 

 

 

 

pUB6/V5 His A说明书

发表于由

 pUB6/V5 His A

型号 载体名称 出品公司 载体用途
VPI0022 pUB6/V5 His A Invitrogen 哺乳动物表达载体
产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Blasticidin
Promoter：UbC
Vector Type：pUB6
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：V5 Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

pUB6/V5-His A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells.

The vectors contain the following elements:

  1. Human ubiquitin C promoter （hUbC） for high-level expression across a broad range of species and cell types （Schorpp et al., 1996; Wulff et al., 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal peptide encoding the V5 epitope and a polyhistidine （6×His） metal-binding tag.
  3. Blasticidin resistance gene （bsd） for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g. COS-1, COS-7）.

The control plasmid, pUB6/V5-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: 
 

Invitrogen pGene v5-His 说明书

发表于由

 

GeneSwith 哺乳动物诱导系统

Invitrogen  pGene v5-His 

 

 

产品参数: 

Mammalian Selection：Zeocin
Constitutive/Inducible：Inducible
Expression Level Tightly controlled （activate with mifepristone）
Sequencing Primer pGene Fwd 
Sequencing Primer Sequence：5’d[CTGCTATTCTGCTCAACCT]3′ 
Protein Tags：6X His，V5

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

Switch-On Expression from the Lowest Basal Levels

The GeneSwitch™ System for inducible mammalian expression is ideal for experiments that require the absolute lowest uninduced expression levels. The expression vector pGene/V5-His provides a minimal promoter, GAL4-E1b, consisting of the binding sites for the yeast Gal4 DNA binding protein followed by the TATA sequence from the Adenovirus E1b promoter. Without additional factors, the GAL4-E1b promoter is transcriptionally silent.

To activate transcription from the GAL4-E1b promoter, the GeneSwitch™ regulatory protein is expressed from a minimal TK promoter on the pSwitch vector. The GeneSwitch™ protein has three functional domains:

  1. Gal4 DNA Binding Domain （Gal4-DBD） to bind the regulatory protein to the GAL4-E1b promoter
  2. Truncated human Progesterone Receptor Ligand Binding Domain （hPR-LBD） that undergoes a conformational change when it binds the progesterone antagonist, mifepristone
  3. Transcription activation domain from the NFØB transcription factor p65 （p6D） to activate transcription from the silent GAL4-E1b promoter

In the absence of mifepristone, the conformation of the hPR-LBD region prevents the GeneSwitch™ regulatory protein from activating transcription from the GAL4-E1b promoter. When mifepristone is added and binds the hPR-LBD region, the GeneSwitch™ regulatory protein assumes a conformation that permits it to stimulate transcription from the GAL4-E1b promoter.

Induction of the GeneSwitch™ System leads to activation of the gene of interest on the vector pGene/V5-His. In addition, four Gal4 binding sites upstream of the minimal HSV TK promoter on pSwitch can bind the pSwitch regulatory protein. Therefore, adding mifepristone up-regulates production of the regulatory protein. The increased levels of the GeneSwitch™ regulatory protein result in induction of the gene of interest from pGene/V5-His to levels that can approach those of viral promoters.

The GeneSwitch™ Kit offers exceptionally low uninduced and high induced expression levels in mammalian cells. The system provides the expression vector pGene/V5-His with the minimal, synthetic GAL4-E1b promoter that is transcriptionally silent until activated. The GeneSwitch™ regulatory protein binds the GAL4-E1b promoter to activate transcription upon the addition of mifepristone. In addition, the GeneSwitch™ protein upregulates its own expression, leading to amplified expression of the gene of interest from pGene/V5-His. pGene/V5-His offers several features that facilitate expression analysis and purification of recombinant proteins in mammalian cells:

  1. C-terminal V5 epitope tag for detection with Anti-V5 Antibodies
  2. C-terminal polyhistidine （6xHis） tag for rapid purification with nickel-chelating resin and detection with Anti-His（C-term） Antibodies
  3. The Zeocin resistance gene for effective selection in both mammalian cells and E. coli