pCDNA4/HisMax A&B&C

 

pCDNA4/HisMax A&B&C

型号 载体名称 出品公司 载体用途
VPI0013 pcDNA4/HisMax B Invitrogen 哺乳动物表达载体
产品参数: 

Mammalian Selection:Zeocin
Sequencing Primer:T7 Fwd
Sequencing Primer Sequence:5’d[TAATACGACTCACTATAGGG]3′ 
Tag:6X His,Xpress

载体抗性: 
氨苄青霉素(Ampicillin)
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pcDNA3.1(-)/myc-His A&B&C说明书

  

pcDNA3.1(-)/myc-His A&B&C

型号 载体名称 出品公司 载体用途
VPI0017 pcDNA3.1(-)/myc-His A Invitrogen 哺乳动物表达载体
VPI0018 pcDNA3.1(-)/myc-His B Invitrogen 哺乳动物表达载体
VPI0019 pcDNA3.1(-)/myc-His C Invitrogen 哺乳动物表达载体

 

产品参数: 

Mammalian Selection:G418,neo
Protein Tag or Fusion:c-Myc Epitope Tag,His Tag (6x His)
Sequencing Primer:T7 Fwd
Sequencing Primer Sequence:5’d[TAATACGACTCACTATAGGG]3′

载体抗性: 
氨苄青霉素(Ampicillin)
质粒图谱: 

 

pcDNA3.1/GS

 

pcDNA3.1/GS

型号 载体名称 出品公司 载体用途
VPI0010 pcDNA3.1/GS Invitrogen 哺乳动物表达载体
产品参数: 

Mammalian Selection:Zeocin
Sequencing Primer:T7 Fwd
Sequencing Primer Sequence:5’d[TAATACGACTCACTATAGGG]3′

载体抗性: 
氨苄青霉素(Ampicillin)
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pcDNA3.1(hyg)+载体说明书

 

pcDNA3.1(hyg)+

型号 载体名称 出品公司 载体用途
VPI0008 pcDNA3.1(hyg)+ Invitrogen 哺乳动物表达载体

 

产品参数: 

Promotor: CMV
5′ Sequencing 1 Primer: T7 Fwd
5′ Sequencing 1 Primer Sequence: 5’d[TAATACGACTCACTATAGGG]3′
Selectable Marker: Hygromycin

载体描述: 

Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori.

说明书: 
 pcDNA3.1(hyg)+ 载体序列
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pcDNA6.2-GWEmGFP-miR negative

 

pcDNA6.2-GWEmGFP-miR negative

型号 载体名称 出品公司 载体用途
VGI0404 pcDNA6.2-GWEmGFP-miR negative Invitrogen Gateway系统

Comments for:

CMV promoter:

attB1 site:

EmGFP:

EmGFP forward sequencing primer site:

5’ miR flanking region:

miR-neg control:

3’ miR flanking region:

attB2 site (c):

miRNA reverse sequencing primer site (c):

TK polyadenylation signal:

f1 origin:

SV40 early promoter and origin:

EM7 promoter:

Blasticidin resistance gene:

SV40 polyadenylation signal:

pUC origin (c):

Spectinomycin resistance gene (c):

Spect ni omycin

5’ miR flanking

 region attB1 EmGFP attB2

pcDNATM 6.2-GW/EmGFP-miR-neg

control plasmid

5759 nucleotides

1 – 588

680 – 704

713 – 1432

1409 – 1428

1492 – 1518

1519 – 1578

1579 – 1623

1652 – 1676

1667 – 1686

1705 – 1976

2088 – 2516

2543 – 2851

2906 – 2972

2973 – 3371

3529 – 3659

3797 – 4470

4540 – 5550

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Invitrogen pcDNA™6 TR说明书

 pcDNA™6⁄TR

载体描述: 

The pcDNA™6⁄TR vector is part of the T-REx™ System, which yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known.

The regulatory vector, pcDNA™6⁄TR, is provided for high-level expression of the tetracycline repressor (TR) protein. This vector expresses the Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express the TR protein.

 

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Invitrogen四环素诱导系统(pCDNA4/HisMax A&B&C)说明书

 Invitrogen四环素诱导系统

VectorspcDNA4/TO/Myc-His ApcDNA4/TO/Myc-His BpcDNA4/TO/Myc-His C

 

产品参数: 

Mammalian Selection:Zeocin
Sequencing Primer:T7 Fwd
Sequencing Primer Sequence:5’d[TAATACGACTCACTATAGGG]3′ 
Tag:6X His,Xpress

载体抗性: 
氨苄青霉素(Ampicillin)
 
 
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