人类原代细胞一览

人类原代细胞一览

推荐的培养基* 产品 接种密度 溶解后传代 标记物** 种群加倍
C-22070 人类心肌细胞 (HCM) 10,000 – 15,000
cells per cm²
P2 Sarcomeric alpha-Actinin+
Slow muscle myosin+
> 15
C-27101 人类软骨细胞(HCH) 10,000 – 20,000
cells per cm²
P2 Differentiation tested > 10
C-22010
C-22011
人类脐静脉内皮细胞(HUVEC) 5,000 – 10,000
cells per cm²
P1 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22010
C-22011
人类脐静脉内皮细胞(HUVEC) 混合 5,000 – 10,000
cells per cm²
P1 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22010
C-22011
人类脐静脉内皮细胞 (HUVEC)
预先筛选
5,000 – 10,000
cells per cm²
P1 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22010
C-22011
人类脐动脉内皮细胞 (HUAEC) 5,000 – 10,000
cells per cm²
P1 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类主动脉内皮细胞 (HAoEC) 5,000 – 10,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类冠状动脉内皮细胞 (HCAEC) 5,000 – 10,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22010
C-22011
人类肺动脉内皮细胞 (HPAEC) 5,000 – 10,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22010
C-22011
人类大隐静脉内皮细胞 (HSaVEC) 5,000 – 10,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类皮肤微血管内皮细胞 (HDMEC)
青少年包皮
10,000 – 20,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类皮肤微血管内皮细胞 (HDMEC)
成年人供者
10,000 – 20,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类皮肤微血管内皮细胞 (HDMEC)
预先筛选
10,000 – 20,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020 人类皮肤血液内皮细胞 (HDBEC)
青少年包皮
10,000 – 20,000
cells per cm²
P2 Podoplanin                                
Prox-1
CD31+
> 15
C-22020 人类皮肤血液内皮细胞 (HDBEC)
成年人供者
10,000 – 20,000
cells per cm²
P2 Podoplanin                                
Prox-1
CD31+
> 15
C-22020 人类皮肤淋巴内皮细胞 (HDLEC)
青少年包皮
10,000 – 20,000
cells per cm²
P2 Podoplanin                                
Prox-1
CD31+
> 15
C-22020 人类皮肤淋巴内皮细胞 (HDLEC)
成年人供者
10,000 – 20,000
cells per cm²
P2 Podoplanin                                
Prox-1
CD31+
> 15
C-22020
C-22022
人类膀胱微血管内皮细胞 (HBdMEC) 10,000 – 20,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类心脏微血管内皮细胞 (HCMEC) 10,000 – 20,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类肺脏微血管内皮细胞 (HPMEC) 10,000 – 20,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-22020
C-22022
人类子宫微血管内皮细胞 (HUtMEC) 10,000 – 20,000
cells per cm²
P2 vWF+                                              CD31+                            
Dil-Ac-LDL uptake+
> 15
C-21060 人类鼻部上皮细胞 (HNEpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 15
C-21060 人类气管上皮细胞 (HTEpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 15
C-21060 人类支气管上皮细胞 (HBEpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 15
C-21070 人类小气道上皮细胞 (HSAEpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 15
C-21010 人类乳腺上皮细胞 (HMEpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 15
C-26001
C-26030
人类肾脏上皮细胞 (HREpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 15
C-26001
C-26030
人类肾皮质上皮细胞 (HRCEpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 15
C-26040 人类胎盘上皮细胞 (HPlEpC) 10,000 – 15,000
cells per cm²
P2 Cytokeratin+ > 5
C-23010 正常人类皮肤成纤维细胞 (NHDF)
青少年包皮
3,500 – 7,000
cells per cm²
P2 CD90+ > 15
C-23020 正常人类皮肤成纤维细胞 (NHDF)
成年人供者
3,500 – 7,000
cells per cm²
P2 CD90+ > 15
C-23020 人类肺脏成纤维细胞 (HPF) 3,500 – 7,000
cells per cm²
P2 CD90+ > 15
C-23020 人类主动脉外膜成纤维细胞 (HAoAF) 3,500 – 7,000
cells per cm²
P2 CD90+ > 10
C-23025 人类心脏成纤维细胞 (HCF) 3,500 – 7,000
cells per cm²
P2 CD90+                                     
Smooth muscle α-actin           
Slow muscle myosin
> 15
C-23020 人类子宫成纤维细胞 (HUF) 3,500 – 7,000
cells per cm²
P2 CD90+ > 15
C-23020 人类绒毛膜间充质成纤维细胞 (HVMF) 3,500 – 7,000
cells per cm²
P2 CD90+ > 15
C-26501 人类毛囊毛乳头细胞 (HFDPC) 5,000 – 10,000
cells per cm²
P2 Alkaline phosphatase+ > 10
C-25010
C-25020
人类肝脏细胞 (HHpC) 150,000 cells per cm² P0 ECOD+                                     
7-HCG/7-HCS+          
Albumin+
 
C-20011 正常人类表皮角质化细胞 (NHEK)
青少年包皮, 单一供者
5,000 cells per cm² P2 Cytokeratin+ > 15
C-20011 正常人类表皮角质化细胞 (NHEK) 青少年包皮,混合 5,000 cells per cm² P2 Cytokeratin+ > 15
C-20011 正常人类表皮角质化细胞 (NHEK)
成年人单一供者
5,000 cells per cm² P2 Cytokeratin+ > 15
C-20011 正常人类表皮角质化细胞 (NHEK)
成年人供者, 混合
5,000 cells per cm² P2 Cytokeratin+ > 15
C-24010 正常人类表皮黑色素细胞 (NHEM)
青少年包皮
5,000 – 10,000
cells per cm²
P2 Mel-5+ > 15
C-24300 正常人类表皮黑色素细胞 (NHEM)
青少年包皮,培养于M2培养基
5,000 – 10,000
cells per cm²
P2 Mel-5+ > 15
C-24300 正常人类表皮黑色素细胞 (NHEM)
成年人供者,培养于M2培养基
5,000 – 10,000
cells per cm²
P2 Mel-5+ > 15
C-27001 人类成骨细胞 (HOB) 10,000 – 20,000
cells per cm²
P2 Alkaline phosphatase+
Mineralization tested
> 10
C-27410
C-27436
C-27438
人类白色前成脂肪细胞 (HWP)
皮下脂肪
5,000
cells per cm²
P2 Differentiation tested > 10
C-27410
C-27436
C-27438
人类白色前成脂肪细胞 (HWP)
内脏脂肪
5,000
cells per cm²
P2 Differentiation tested > 10
C-23060
C-23061
人类骨骼肌细胞
(SkMC)
3,500 – 7,000
cells per cm²
P2 Sarcomeric Myosin+
Differentiation tested
> 15
C-22062 人类主动脉平滑肌细胞
(HAoSMC)
7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类冠状动脉平滑肌细胞 (HCASMC) 7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类肺动脉平滑肌细胞
(HPASMC)
7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类脐带动脉平滑肌细胞 (HUASMC) 7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类气管平滑肌细胞 (HTSMC) 7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类支气管平滑肌细胞(HBSMC) 7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类膀胱平滑肌细胞 (HBdSMC) 7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类前列腺平滑肌细胞 (HPrSMC) 7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15
C-22062 人类子宫平滑肌细胞
(HUtSMC)
7,500 – 10,000
cells per cm²
P2 Smooth muscle alpha-Actin+ > 15

* 该表格中的培养基为即用型。培养基试剂盒信息,请参见第4章。
** CD 31, Prox-1 和肾小球上皮细胞整合膜蛋白抗体均可以从 PromoKine购买。

 

  • 心肌细胞
  • 软骨细胞
  • 内皮细胞(大血管)
  • 内皮细胞(微血管)
  • 上皮细胞
  • 成纤维细胞
  • 毛囊毛乳头细胞
  • 肝细胞