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Homer 1 (VesL 1, Syn 47)
A post synaptic density (PSD) protein involved in the targeting of glutamate receptors
Fact Sheet Cat. No. 160 003
| 多克隆抗兔抗体1 | |
|---|---|
| Cat. No. 160 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications | WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 500 up to 1 : 1000 (see remarks!) image IHC: 1 : 200 image IHC-P: 1 : 500 image ELISA: yes (see remarks) |
| Immunogen | Recombinant protein of human homer (aa 1 – 186). |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Specific for Homer 1. Cross-reactivity of the serum to Homer 2 and 3 was removed by pre-adsorption with Homer 2 (aa 1 – 176) and Homer 3 (aa 1 – 177). The Homer 1-specific sequence aa 121 – 186 was used for affinity purification. According to Soloviev et al. (2000), aa 1 – 180 are present in isoforms a, b, c and d. |
| Remarks | ICC: PFA fixation recommended. Methanol fixation leads to a complete loss of the signal. ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 160 011 as capture antibody (protocol for sandwich-ELISA). |
相关产品信息如下：
| 货号 | 品名 | 规格 | 品牌 |
| 104 211 | Synaptobrevin 2 | 50μg | SYSY |
| 111 011 | SNAP 25 human, rat, mouse, vertebrates | 50µg | SYSY |
| 131 103 | VGAT lumenal domain | 50μg | SYSY |
| 135 311 | VGLUT 1 | 100μg | SYSY |
| 135 416 | chicken polyclonal, purified IgY | 50μg | SYSY |
| 160 003 | Homer 1 (VesL 1, Syn 47) | 50ug | SYSY |
| 182 011 | GluA 1 | 100μg | SYSY |
| 202 003 | m6A | 50ug | SYSY |
| 224 103 | GABA-A receptor alpha2 | 50μg | SYSY |
| 226 003 | c-Fos | 50μg | SYSY |