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InstantBlue 快速蛋白染色液 是已配制好可直接使用的蛋白凝胶电泳染色剂，速度快,灵敏度高,无毒,效果好。
Expedeon 的 InstantBlue 是*真正的实现一步考马斯凝胶染色。只需简单的从电泳槽中取出凝胶、放入染色剂中，就可离开了。
无需洗涤、微波加热或脱色。所有条带将在 15 分钟内可见。
凝胶可以无限期留在 InstantBlue试剂内, 只有蛋白质被染色，不会增加背景染色。
InstantBlue 是无毒的并且与质谱分析兼容。
超快 15分钟内即可看到结果，1小时后完成 简单 一步即可
染色前不需要清洗 染色前不需要固定 不需要加热/微波 不需要脱色
灵敏 ≥5ng 蛋白/带
可用于质谱 Edman Sequencing 安全 无毒 无甲醇 废液可直接排放
InstantBlue – ILSB1L
InstantBlue is a ready to use Coomassie protein stain for polyacrylamide gels.
Its unique mechanism of action stains proteins in 15 minutes while leaving
a clear background eliminating the need to fix, wash or destain.
InstantBlue is formulated for safe use and easy disposal.
• Ultrafast 15 minute staining
• Simple one step protocol
• Safe usage and disposal
InstantBlue Features & Benefits
• Ultra-fast staining – Results in 15 minutes or less
• Single step procedure – No washing, fixing, microwaving or destaining
• High sensitivity – 5ng bands detectable*
• Clear background – High signal/noise ratio
• Flexible – No over staining
• Quantitative – Batch to batch consistency
• Efficient – Only 25ml per gel
• Safe composition – Non-toxic. No fume hood or solvent disposal required
• Methanol free – No gel shrinkage or protein methylation
• Acetic acid free – No protein aceylation
• MS Compatible 1 – Destainable. No residual methylation or acetylation
InstantBlue is ready for use straight out the bottle and comes in convenient pre-mixed 1 litre volumes.
Its unique patented mechanism begins to stain in moments and results are achieved within 15 minutes.
Some premade and traditional home-made Coomassie R-250 stains can take 3 hours or more to fully stain gels.
There’s no need to wash, fix, microwave or destain gels when using InstantBlue.
Homemade stains often require all these steps and most premade solutions require at least one.
InstantBlue uses a one-step protocol and can be fully destained using standard procedures.
nstantBlue’s acetic acid free formulation doesn’t methylate or acetylate protein during
staining making it fully compatible with mass spectrometry.
Unparalleled sensitivity allows protein levels as low as 5 ng per band to be detected (BSA)
when staining overnight. This is thanks to InstantBlue’s protein specific staining which
gives a clear background, improving the signal/noise ratio of the stain. Gels can be left
in InstantBlue for weeks and still come out with a crystal clear background,
all without the need for destaining.
InstantBlue is non-toxic and sink disposable due to its methanol free composition.
Other stains require solvent disposal procedures and can require microwaving which
produces dangerous fumes. InstantBlue’s effective staining and safe composition means
there’s no need for microwaving, solvent disposal, or fume hoods.
The lack of methanol also means no gel shrinkage.
Economical staining with only 25ml per mini-gel allows you to use less InstantBlue. Homemade solutions and some pre-mixed solutions require about 50-100ml of stain per gel. InstantBlue will stain at least 40 mini-gels per bottle.
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