Hampton 蛋白结晶优化试剂 Optimization Screens

Hampton 蛋白结晶优化试剂 Optimization Screens

1、Solubility & Stability Screen  蛋白特异性试剂优化溶解性和稳定性
Protein specific agents for optimization of solubility & stability  
2、Solubility & Stability Screen 2   离子缓冲液
Buffer, pH & ionic strength screen for optimization of solubility & stability
3、Slice pH    PH缓冲液
pH & buffer screen for optimization of crystals or sample solubility & stability
4、Additive Screen  96孔板添加剂试剂盒
96 compound screen for optimization of crystal size & quality
5、Silver Bullets • Silver Bullets Bio
Small molecule additive screen for optimization of crystal size & quality
6、Detergent Screen
Detergent screen for optimization of crystals or sample solubility & stability
7、Ionic Liquid Screen
Ionic liquid additive screen for optimization of crystal size & quality
8、StockOptions Buffer Kits
Titrated buffer sets for optimization of crystals or sample solubility & stability
9、StockOptions Polymer
n of crystal size & quality
10、StockOptions Salt
Salt reagent kit for optimization of crystal size & qualit
11、Polymer reagent kit for optimizatioy
Proti-Ace
In situ proteolysis kit protein for crystallization screening & optimization
12、Reductive Alkylation
Reductive alkylation kit for crystallization screening & optimization
13、Silica Hydrogel Kit
pH neutral silica hydrogel matrix crystallization
14、Low Melting Agarose
Agarose gel matrix crystallization
15、CryoPro
Water soluble cryoprotectant reagent set
16、Heavy Atom Screens
Heavy atom kits for multiple isomorphous replacement
17、I3C Phasing Kit
Produce heavy atom derivative of biological macromolecules for phasing

Hampton坐滴蛋白结晶板 3 Well Crystallization Plate (Swissci)

Hampton坐滴蛋白结晶板     3 Well Crystallization Plate (Swissci)
3 Well Crystallization Plate (Swissci)
Applications:Sitting drop crystallization  应用于坐滴结晶
该坐滴结晶板的特点:Features

Lens effect drop wells for enhanced optics
Drop volume: 50 nl to 5 碌l
Micro-numbering alongside drop volumes
Reservoir volume: 20 to 40 碌l
ANSI/SLAS 1-2004 Standard
Drop well allows easier crystal harvesting
Three drop wells per reservoir
Midi profile or Low Profile for easier storage
UV compatible (UVP)

Hampton坐滴蛋白结晶板3    Well Crystallization Plate (Swissci)
Description
The SWISSCI 3 Lens Crystallization Plate is an ideal product for protein crystallization presented in a 96-well plate format.
该结晶板是用于在96孔板呈现蛋白质结晶的理想产品
The plate was developed in collaboration with very experienced protein crystallographers. It is the result of many years of experience in successful robotic high-throughput crystallization and combines many of the necessary features not earlier available to the crystallographer.
该板协同经验非常丰富的蛋白质晶体学家开发。这是多年的成功的机器人高通量结晶经验的结果,并结合了许多不早提供给晶体学家必要的功能。
The SWISSCI 3 Lens crystallization Plate offers unique properties that make it ideal for both nanoliter crystallization screening and mircoliter optimization alike. Made from optically superior polymer (UVP) and with a new design of the wells, the plate allows easy crystal viewing and retrieval.The Advantages of the 3 Lens Crystallization Plate    优点:Easy crystal retrieval   易结晶
Raised wide wells make the crystal mounting especially easy.Easy viewing
The wells are a wide conical shape and have a lens effect for perfect illumination. The micro-numbering ensures you will never get lost again (visible by microscope). The optically superior polymer is UV transmissible and may be used to differentiate between salt and protein crystals.Better sealing
Wide partition walls between the wells give plenty of area for good sealing with tape. No central bending occurs in this very robust structure. Excellent long term storage – no sample evaporation.Wide range of volumes
Typical volumes are 20-40 µl of reservoir and 50 nl-5 µl drop size. The 288 optical wells offer three times the number of experimental constructions.ANSI/SLAS 1-2004 Standard
The plates are designed to the 96-well ANSI/SLAS 1-2004 standards for all common holders and external numbering (A-H, 1-12)with corner location make the plate easy to use in a robotic sampler. The 3 Lens plate is suitable for centrifugation. The unique 3 drop protein crystallization plate offers a new way of sitting-drop crystallography. The 288 wells are optically perfect designed to observe crystals under a microscope.3 Lens plate features
Easy to fill 96-lens structure – typically 30 microlitres per buffer lens. Standard ANSI/SLAS 1-2004 form for automation. Optically perfect wells with lens effect for a better view with microscope. Microscopic identifier within wells, simplifying the orientation under the microscope. Maximum volume of the buffer reservoir is 40 microlitres. Grown crystals are easy to identify and to remove from lens due to a low-binding polymer. Plate with 3 lenses for each sample, better growing security with triplicates or the ability to use lens two and lens three as mixing stations. Lenses fill without micro-droplets jumping out due to static effects. Wells can be individually sealed with a perfectly flat upper surface – there is a large land area designed to ensure integrity of each lens section. The profile allows for easier storage. Low volume buffer well enables savings on reagents. Triplicates allow for 288 constructs per plate.3 Well Midi Crystallization Plate (Swissci 3W96TUVP)
3 Well Low Profile Crystallization Plate (Swissci 3W96TLP-UVP)
Hampton坐滴蛋白结晶板  3 Well Crystallization Plate (Swissci)
Sitting drop crystallization

CAT NO NAME DESCRIPTION
HR3-123 3 Well Midi Crystallization Plate (Swissci) 10 plate case
HR3-125 3 Well Midi Crystallization Plate (Swissci) 40 plate case
HR3-205 3 Well Low Profile Crystallization Plate (Swissci) 10 plate case
HR3-206 3 Well Low Profile Crystallization Plate (Swissci) 40 plate case

Hampton 水晶透明密封带Crystal Clear Sealing Tape

Hampton 水晶透明密封带Crystal Clear Sealing Tape
Crystal Clear Sealing Tape
3 inch, 1.88 inch, 1.88 inch with dispenser, 0.75 inch with dispenser (left to right)
Crystal Clear Sealing Tape
0.75 inch Crystal Clear Sealing Tape
Applications  应用
Sealing tape used to seal sitting drop crystallization experiments
Description
These optically clear tapes are compatible with protein crystallization reagents. The polypropylene Crystal Clear Sealing Tapes use acrylic solvent based adhesives which are compatible with aqueous crystallization reagents.
Catalog number HR3-511 is a 1.88 inch (48 mm) wide, 3 mil tape on a 43.7 yard (40 M) roll with a 1.5 inch core and is supplied with a green dispenser / cutter.Catalog number HR4-511 is a 1.88 inch wide, 3 mil tape on a 60 yard roll with a 3 inch core and no dispenser / cutter.Two strips of the HR3-511 or HR4-511 will seal a Corning, Cryschem, CrystalClear Strip, Greiner, Intelliplate, Linbro, Swissci (MRC), VDX and VDXm plate.Catalog number HR4-506 is a 3 inch wide, 3 mil tape on a 54.86 yard roll with a 3 inch core and no dispenser / cutter.One strip of the HR4-506 will seal a Corning, Cryschem M, CrystalClear Strip, Greiner, Intelliplate, Swissci (MRC) and VDXm plate.Crystal Clear Mini Sealing Tape HR4-508 is a 0.75 inch (19 mm) wide, 3 mil tape on a 650 inch (16.5 M) roll and is supplied with a clear dispenser / cutter. The tape is useful for resealing or patching wells after mounting crystals. The tape is also wide enough to seal a single row of a Cryschem S or Cryschem M plate.See chart for tape and plate compatibility.

Hampton Natrix • Natrix 2 • Natrix HT

Hampton Natrix • Natrix 2 • Natrix HT

Applications
  Primary biased sparse matrix crystallization screen for nucleic acids & protein/nucleic acid complexes  主要用于核酸和蛋白质/核酸复合物的删选
Features

Nucleic acid sparse matrix screen
Sparse matrix formulation efficiently samples salts, polyols, polymers, organics, additives & pH
pH range 5.6 – 8.5
Tube or Deep Well block format
 Description
Natrix, Natrix 2 and Natrix HT are based upon published reagent formulations for the crystallization of nucleic acids and protein-nucleic acid complexes. A variety of hammerhead ribozymes and other ribozymes, RNAs, DNAs, RNA-drug complexes, and RNA-protein complexes have been crystallized using the Natrix protocols.By using sparse matrix sampling technology, The Natrix kits allow one to quickly test wide ranges of pH, salts, and precipitants using a very small sample of nucleic acid.
Natrix screens are unique in that rather than relying solely on the traditional nucleic acid precipitant MPD, Natrix screens also utilize Polyethylene glycols (PEGs) in a variety of molecular weights (200, 400, 4,000, 8,000) as well as 2-Propanol, Polyethylene glycol monomethyl ether (PEG MME), and 1,6-Hexanediol. Many of the polymeric and low molecular weight organic precipitants are combined with various monovalent salts as precipitating agents. This combination of salts and low molecular weight organics and polyalcohols, as well as the utilization of varying chain length PEGs, has proven to be a successful combination for producing nucleic acid and protein-nucleic acid complex crystals.
Natrix contains 48 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by William Scott in 1995.
Natrix 2, an extension of Natrix, contains 48 unique reagents, 10 ml each. Natrix 2 is a biased sparse matrix screen based on extracting patterns from crystallization data as well as reagent formulations first described by Berger et al in 1996.
Natrix HT contains 1 ml of each reagent from Natrix and Natrix 2 in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
Natrix • Natrix 2 • Natrix HT
Natrix • Natrix 2 • Natrix HT
View Full Size
Natrix • Natrix 2 • Natrix HT

 

CAT NO NAME DESCRIPTION
HR2-116 Natrix 10 ml, tube format
HR2-117 Natrix 2 10 ml, tube format
HR2-131 Natrix HT 1 ml, Deep Well block format

Hampton蛋白结晶产品目录

Hampton蛋白结晶产品目录

产品编号 中文品名 英文品名
HR4-126 11 刀片替换装 Replacement #11 Blade
HR4-904 CrystalCap Magnetic Vial CrystalCap™, Vial only
HR4-729 CrystalWand Magnetic, Straight CrystalWand™ Magnetic, Straigh
HR4-837 Micro-Tools II Set Micro-Tools II™ Set
HR4-811 Micro-Tools Set Micro-Tools™ Set
HR3-172 VDX 24孔板,带密封剂 VDX™ Plate, with sealant
HR4-902 带磁力底座 CrystalCap™, without Vial
HR4-733 带磁力底座 CrystalCap™, with Vial
HR4-745 带铜管磁力底座 CrystalCap™ Copper 18 mm, with
HR4-708 冻存套 CryoSleeve™
HR3-217 硅化方形盖玻片22mmx0.22mm 22 mm x 0.22 mm Siliconized Sq
HR4-671 夹钳-弯 Vial Clamp™ – Curved, 45°/135°
HR4-670 夹钳-直 Vial Clamp™ – Straight
HR4-316 Super Glue
HR4-506 胶带 3 inch wide x 55 yard Crystal
HR3-083 结晶板 2孔 MRC 2 Well Crystallization Pla
HR3-125 结晶板 3孔 3 Well Crystallization Plate (
HR4-217 晶体探针 Crystal Probe
HR4-662 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR4-673 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR4-674 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR4-675 泡沫保温瓶 Spearlab Cryogenic Foam Dewar
HR1-008 液氮罐 CX100 (含运输箱) CX100 Cryo-Express Dry Shipper
HR2-912-37   Crystal Screen 2™ #37
HR2-501   50% v/v Jeffamine® M-600® pH 7
HR2-509   1.0 M BICINE
HR2-517   100% Polyethylene glycol 300
HR2-523   50% w/v Polyethylene glycol 1,
HR2-525   50% w/v Polyethylene glycol 1,
HR2-529   50% w/v Polyethylene glycol 4,
HR2-533   50% w/v Polyethylene glycol 6,
HR2-537   1.0 M Magnesium formate dihydr
HR2-541   3.5 M Ammonium sulfate
HR2-545   2.0 M Lithium sulfate monohydr
HR2-547   7.0 M Sodium formate
HR2-549   1.6 M Sodium citrate tribasic
HR2-551   4.0 M Sodium phosphate monobas
HR2-557   2.0 M Calcium chloride dihydra
HR2-563   1.0 M Zinc acetate dihydrate
HR2-102   StockOptions™ Hepes
HR2-103   StockOptions™ Bis-Tris Propane
HR2-104   StockOptions™ Citric Acid
HR2-106   StockOptions™ Bis-Tris
HR2-107   SaltRx™ 1
HR2-109   SaltRx™ 2
HR2-110   Crystal Screen™
HR2-112   Crystal Screen 2™
HR2-114   MembFac™
HR2-116   Natrix™
HR2-117   Natrix™ 2
HR2-118   Nucleic Acid Mini Screen™
HR2-126   PEG/Ion Screen™
HR2-235   StockOptions™ Sodium Citrate
HR2-237   StockOptions™ Tris Hydrochlori
HR2-239   StockOptions™ Sodium Cacodylat
HR2-241   StockOptions™ pH
HR2-245   StockOptions™ Salt
HR2-249   StockOptions™ Succinic Acid
HR2-320   Seed Bead™
HR2-406   Detergent Screen HT™
HR2-428   Additive Screen™
HR2-434   Reductive Alylation Kit
HR2-442   Heavy Atom Screen™ Pt
HR2-444   Heavy Atom Screen™ Au
HR2-446   Heavy Atom Screen™ Hg
HR2-448   Heavy Atom Screen M1™
HR2-450   Heavy Atom Screen M2™
HR2-082   PEGRx™ 1
HR2-084   PEGRx™ 2
HR2-096   Silver Bullets™
HR2-098   PEG/Ion 2 Screen™
HR4-315   CrystalWand™ Magnetic, 45° McM
HR4-350   Large Applicator (200 & 350 µl
HR4-625   Mounted CryoLoop™ – 20 micron,
HR4-637   CryoTong™ 18 mm (standard 110
HR4-709   CryoCane™ 5 Vial Holder
HR4-711   CryoCane™ 6 Vial Holder
HR2-211   Grid Screen™ Ammonium Sulfate
HR2-213   Grid Screen™ PEG 6000
HR2-227   StockOptions™ Polymer
HR2-233   StockOptions™ Sodium Acetate
HR4-931   CryoLoop™ – 20 micron, 0.2 – 0
HR4-945   Mounted CryoLoop™ – 20 micron,
HR4-947   Mounted CryoLoop™ – 20 micron,
HR4-953   Mounted CryoLoop™ Sampler – 20
HR4-955   Mounted CryoLoop™ – 20 micron,
HR4-957   Mounted CryoLoop™ – 20 micron,
HR4-959   Mounted CryoLoop™ – 20 micron,
HR4-961   Mounted CryoLoop™ – 20 micron,
HR4-963   Mounted CryoLoop™ – 20 micron,
HR4-970   Mounted CryoLoop™ – 20 micron,
HR4-971   Mounted CryoLoop™ – 20 micron,
HR4-989   CryoLoop™ – 10 micron, 0.3 – 0
HR2-128   Crystal Screen Lite™
HR2-138   Additive Screen HT™
HR2-140   PCT™ – Pre-Crystallization T
HR4-313   Mounted CryoLoop™ – 20 micron,
HR2-601   100% Polyethylene glycol 200
HR2-607   50% w/v Polyethylene glycol 10
HR2-609   30% w/v Polyethylene glycol 20
HR2-611   100% Polyethylene glycol monom
HR2-613   50% w/v Polyethylene glycol mo
HR2-615   50% w/v Polyethylene glycol mo
HR2-625   6.0 M 1,6-Hexanediol
HR2-637   5.0 M Sodium chloride
HR2-723   1.0 M BICINE pH 9.0
HR2-755   100% Tacsimate™ pH 7.0
HR2-761   3.0 M DL-Malic acid pH 7.0
HR2-801   TCEP hydrochloride
HR2-821   2.0 M Magnesium sulfate heptah
HR2-829   100% Tacsimate™ pH 8.0
HR2-857   4.0 M TMAO
HR2-859   100% Polyethylene glycol 600
HR2-910-08   Crystal Screen™ #08
HR2-144   Index™
HR4-993   Mounted CryoLoop™ Sampler – 10
HR4-995   Mounted CryoLoop™ – 10 micron,
HR4-999   Mounted CryoLoop™ – 10 micron,
HR6-146   1.0 mm Quartz Capillary
HR8-034   Cryo Puck Carrier
HR8-035   Porcupine Push Tool
HR8-036   Puck Wand
HR8-037   Cryo Puck Base Removal Platfor
HR8-038   Angled Cryo Puck Tong
HR8-039   Cryo Puck Dewar Loading Tool
HR8-114   CrystalCap HT™, Vial only
HR8-133   Seeding Tool
HR8-173   CrystalCap™ Copper HT, 0.025 –
HR8-174   CrystalCap™ Copper HT, 0.05 –
HR8-175   CrystalCap™ Copper HT, 0.1 – 0
HR8-176   CrystalCap™ Copper HT, 0.2 – 0
HR2-575   1.0 M Sodium cacodylate trihyd
HR2-581   1.0 M Hydrochloric acid

Hampton 银弹 Silver Bullets

Hampton 银弹 Silver Bullets

Applications

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability
Features

Developed at Hampton Research
Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

Description
Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3
Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3
X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.
Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests
The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:
•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal
The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ºC, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.
Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.
Silver Bullets reagent volume is 0.5 ml (each well).
ThermoFluor is a registered trademark of Johnson & Johnson.

Hampton  Silver Bullets Bio

Silver Bullets • Silver Bullets Bio
Silver Bullets • Silver Bullets Bio
上海金畔生物作为Hampton蛋白结晶产品的代理商,竭诚为您服务,欢迎新老客户咨询。
Applications 应用

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

 

Developed at Hampton Research
Two sets of 96 reagents, composed of more than 1,090 chemicals, of which more than 400 are uniqu

Please note, the Silver Bullets Bio Screen is temporarily unavailable as a new formulation is in development
 

低温泡沫杜瓦瓶 Spearlab Cryogenic Foam Dewars

低温泡沫杜瓦瓶 Spearlab Cryogenic Foam Dewars
Spearlab Cryogenic Foam Dewars
Spearlab Dewars
Spearlab Cryogenic Foam Dewars
HR4-662 Cryogenic Foam Dewar (500 ml) – Small  500ml规格
Spearlab Cryogenic Foam Dewars
HR4-675 – Spearlab Cryogenic Foam Dewar (2 liter)  2L规格
低温泡沫杜瓦瓶 Spearlab Cryogenic Foam Dewars
Applications 应用
    Cryocrystallography 蛋白结晶学

Features
  Each dewar is supplied with a lid  每个杜瓦瓶都配有盖子
  Proprietary foam construction (USPTO # 7,971,744)
  Mini (130 ml), Small (500 ml), Standard (800 ml), Large (1400 ml) and Tall (1800 ml)
   Reduced ice formation on dewar lip
  Easier to dry
   Foam construction reduces slipping

Description
Spearlab FD-130 Cryogenic Foam Dewar (130 ml) – Mini : Small, hexagonal outside, hand held foam dewar, maximum volume 175 ml. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 2 inches in diameter by 3.5 inches deep, so that it easily holds 130 ml and a maximum volume of 175 ml of liquid nitrogen. The HR4-676 has an overall height of approximately 4.5 inches without cover and the width is 3.5 inches at the top and 3.75 inches on the bottom (measured on the flats of the hexagon). Circular cover is approximately 0.5 inches tall x 3.5 inches diameter.
Spearlab Cryogenic Foam Dewar (500 ml) – Small: The small foam dewar shape is circular, with a protruding handle, so that it resembles a large teardrop. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 4.5 inches in diameter by 2.8 inches deep, so that it easily holds 500 ml of liquid nitrogen.
Spearlab FD-800 Cryogenic Foam Dewar (800 ml) – Standard: The standard foam dewar shape is circular, with a protruding handle, so that it resembles a large teardrop. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 5.8 inches in diameter by 2.8 inches deep, so that it easily holds 800 ml of liquid nitrogen.
Spearlab FD-1400 Cryogenic Foam Dewar (1400 ml) – Large: The large foam dewar shape is circular, with a protruding handle, so that it resembles a large teardrop. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 6.3 inches in diameter by 3.8 inches deep, so that it easily holds 1400 ml of liquid nitrogen. Overall height is approximately 6 inches without cover and the width is 9 inches on the short axis and 11 inches on the long axis. Cover approximately 0.5 inches tall x 9 inches diameter.
Spearlab FD-1800 Cryogenic Foam Dewar (1800 ml) – Tall: The tall foam dewar shape is a tapered octagon on the outside with a cylindrical interior. The tapered octagon features a wide, stable base. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 3.5 inches in diameter by 12.5 inches deep, so that it holds approximately 1,800 ml of liquid nitrogen. The outside dimensions of the tall dewar are 15.25 inches tall with a 6.25 inches wide top and a 7.75 inches wide base (measured on the flats of the octagon). The cover is 6.5 inches in diameter and 0.5 inches thick.
The patented (USPTO # 7,971,744) design of the Spearlab Cryogenic Foam Dewar makes it easy to handle and safer to use than a traditional low profile glass dewar. Also, because of its lower thermal mass, a foam vessel will cause less boil off when it is filled with liquid nitrogen. Additionally, the dewar will accumulate less frost during regular use. The end result is that less liquid nitrogen is consumed.
The dewars are made from cross-linked polyethylene foam. A density of 4 pounds per cubic foot is used for the 130 ml and 800 ml dewars. A density of 2 pounds per foot is used for the 1400 ml and 2 liter dewars. The higher density foam has significantly higher mechanical strength and stiffness. The lower density foam has slightly lower thermal conductivity. Like most lightweight insulation materials, the thermal conductivity of the foam is predominantly determined by the thermal conductivity of air, which eventually permeates the pores of the foam material.

CAT NO NAME DESCRIPTION
HR4-676 Cryogenic Foam Dewar (130 ml) – Mini each
HR4-662 Cryogenic Foam Dewar (500 ml) – Small each
HR4-673 Cryogenic Foam Dewar (800 ml) – Standard each
HR4-674 Cryogenic Foam Dewar (1400 ml) – Large each
HR4-675 Cryogenic Foam Dewar (2 liter) – Tall each

Hampton热销产品

Hampton Research是 美国最权威的蛋白结晶试剂盒提供商,产品齐全,性价比高。
在生命科学领域,上海金畔生物为业界提供了丰富的实验室和生物医药生产研发的产品。庆祝上海金畔生物正式成为Hampton的签约代理商,在这里要感谢广大客户多年来对上海金畔生物科技有限公司的支持和厚爱,我们将一如既往的为广大客户带来Hampton高品质的产品和服务,欢迎广大新老客户来电咨询。‘
美国Hampton Research公司位于加利福尼亚州,是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材,同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线,不仅为全世界晶体研究人员的工作带来了便捷,而且也受益于科研人员的回馈不断发展自己,目前已成为晶体研究领域最受信赖的品牌之一。
Hampton research主要产品:
Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液
Optimize Reagents 结晶级别盐类,聚合物,有机溶液或缓冲液
Crystallization Plates, Hardware & Accessories 结晶板(悬滴法和坐滴法,微量透析,微配液,自由界面扩散,凝胶以及无容器悬浮法)及其他配件
Cryocrystallography 低温晶体学耗材如 Crystalcap system,cryoloops
Goniometer Heads&Supplies 测角器头和配件
Protein Crystallization Standards 结晶级别标准蛋白
Custom Shop Crystallization Reagents 定制试剂盒和结晶溶液内的单一组分
StockOptions Kits 内含盐类,缓冲液和冷冻液的简便型试剂盒
Tools,Seeding&Resin 结晶用探针,结晶笔,seeding法结晶工具
Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂
Hampton热销产品

  • StockOptions pH Buffer Kit HR2-241
    Crystallization grade buffer stocks for crystal screening, optimization, & production
    Crystal Screen Cryo HR2-122
    Primary screen with cryo for proteins, soluble peptides, nucleic acids, & water soluble small molecules
  • Natrix HR2-116
    Primary biased sparse matrix crystallization screen for nucleic acids & protein/nucleic acid complexes
  • MembFac HR2-114
    Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility
  • SaltRx HR2-136
    Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules
  • Grid Screens HR2-248
    Primary or secondary, salt, polymer, organic and pH grid crystallization screen for biological macromolecules
  • PEG/Ion HR2-126
    Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules
  • PCT™ Pre-Crystallization Test HR2-140
    Determine the appropriate sample concentration for crystallization screening
  • Index HR2-144
    Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules
  • Crystal Screen HR2-110
    Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules;Sparse matrix additive screen
  • PEGRx HR2-082
    Primary and secondary, polymer and pH based crystallization screen for biological macromolecules
  • Additive Screen HR2-428
    Manipulate sample-sample & sample-solvent interactions to improve crystals or alter sample solubility
  • Detergent Screen HR2-408
    Prevent and manipulate non-specific aggregation due to hydrophobic interactions
  • CryoPro HR2-073
    Water soluble cryoprotectant reagent set designed for the cryopreservation of biological macromolecular crystals
  • StockOptions Salt HR2-245
    Crystallization grade salt reagent stock solutions for screen formulation and optimization
  • Siliconized Glass Cover Slides HR3-217
    Hanging, sitting or sandwich drop crystallization
  • Intelli-Plate 96 HR3-185
    蛋白结晶座滴板
  • CrystalCap HR4-733
  • CryoLoop HR4-623

    • CryoTong™ – Long HR5-104
      Crystal transfer under cryo temperature
    • CrystalWand™ Magnetic HR4-729
      CrystalCap Magnetic & CrystalCap Copper Magnetic handling tool
    • Vial Clamp™ – Straight HR4-670
      Vial support and manipulation
    • CrystalCap™ Holder HR4-707
    • CryoCane HR4-709
      Organized storage of CrystalCaps

Hampton Polyethylene glycol 4,000

Hampton Polyethylene glycol 4,000

Applications
Crystallization grade Polyethylene glycol for formulating screens or for optimizatio
Features
Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description
Polyethylene glycol 4,000
Synonym: PEG 4,000
H(OCH2CH2)nOH
Mr 3,500-4,500
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3500000
MDL Number MFCD00081839
PubChem Substance ID 24890217
Melting point (Starting Material) 58.0-61.0°C
Aldehyde None detected
Peroxide None detected

HR2-529 Measured pH range: 6.9 – 9.5 at 25°C
HR2-529 Measured Conductivity Range: 35.3 – 46.9 µS/cm at 25°C
HR2-529 Measured Refractive Index Range: 1.40172 – 1.40279 at 20°C

CAT NO NAME DESCRIPTION
HR2-605 Polyethylene glycol 4,000 Flake – 500 g
HR2-529 Polyethylene glycol 4,000 50% w/v solution – 200 ml

Hampton Solubility & Stability Screen

Hampton Solubility & Stability Screen

Solubility & Stability Screen(溶解度和稳定性的屏幕)
应用:溶解性和稳定性屏幕的设计,以协助识别解决方案的条件,促进蛋白质的溶解性和稳定性,并尽量减少蛋白质沉淀。
Solubility & Stability Screen
Solubility & Stability Screen
Solubility & Stability Screen
Solubility & Stability Screen HR2-072
Applications

A solubility screen, a stability screen, an additive screen for protein assays including ThermoFluor and crystallization
Preformulation screening

 

Compatible with Thermofluor , Differential Scanning Fluorimetry (DSF), and Protein Thermal Shift assays
96 sterile filtered reagents
17 classes of reagents and excipients
Highly concentrated (10x) reagent formulation
Developed at Hampton Research

Features
Methodologies

Thermal shift assay
Dynamic Light Scattering
Use Solubility & Stability Screen along side Slice pH and Solubility & Stability Screen 2 to optimize sample buffer
Description

Solubility & Stability Screen is designed to assist in the identification of solution conditions which promote protein solubility and stability, and minimize protein precipitation. Solubility & Stability Screen is a solubility screen, a stability screen, and may also be used as an additive screen in the presence of a crystallization reagent.
The Hampton Research Solubility and Stability Screen can evaluate protein solubility, stability and crystallization in the presence of 94 different chemical additives sampling 17 different classes of reagents plus two controls.
Protein solubility and stability are universally required in a wide range of applications, including general biochemical studies, the preparation of proteins in pharmaceuticals, structural biology and crystallization.1-11The preparation of a concentrated, soluble and stable protein sample can often be a difficult task as proteins often aggregate, precipitate or denature.
Thermofluor (also known as Differential Scanning Fluorimetry, DSF and Protein Thermal Shift) provides a rapid, convenient and effective means of identifying subsaturating solution conditions that may also correlate with protein heterogeneity and poor crystallization outcomes.12
Protein solubility and stability is affected by many different chemical factors including pH, buffer type, chemical additives and excipients. pH and buffer type are dominant protein solubility and stability variables and can be evaluated and optimized using the Hampton Research Slice pH kit HR2-070. Slice pH evaluates protein solubility, stability and crystallization versus 20 different buffers over the pH range 3.5-9.6. Chemical additives influencing protein solubility and stability can be evaluated using the Solubility & Stability Screen.
It is widely accepted that protein solubility and stability can be increased by the use of chemical additives.5,15Seventeen classes of reagents are sampled by the Solubility & Stability Screen and each of these classes has been reported as important in improving sample solubility and stability.2-11
The Solubility & Stability Screen is a set of 94 high purity reagents formulated in high purity water (NCCLS/ASTM Type 1+) at 25°C and are 0.22 micron sterile filtered. The 94 solubility and stability reagents are formulated at 2 to 10 times their recommended working concentration. The remaining two reagents are water and a negative (TCA) control. A water control demonstrates the effect of diluting sample as well as sample buffer concentration. TCA, the negative control, demonstrates total sample precipitation, loss of sample solubility and loss of sample stability. The effects of the Solubility & Stability reagents can be compared with this negative control to assist in the identification of reagents promoting sample solubility and stability. 500 microliters of each reagent is supplied in a sterile 96 well polypropylene Deep Well block. The Solubility & Stability Screen reagents are compatible with the sitting or hanging drop vapor diffusion, microbatch, free interface diffusion, sandwich drop vapor diffusion, and dialysis crystallization methods utilizing water soluble reagents.