5-diagnostics 5D-30805说明书
5-Diagnostics总部位于瑞士，在美国拥有强大的基础，并采用集中管理的止血代表和产品专家的网络。在满足包括临床，研究和行业团体在内的广泛客户需求方面，这些公司均具有多年的专业知识。我们决心通过在范围内采购和制造好的研究和临床产品，为诊断技术人员和科学家带来创新。我们对高质量的承诺体现在我们快速扩展的*和专业化验与组分产品组合中。
5-Diagnostics是IVD市场的新名称。我们向止血研究和诊断市场提供创新的IVD系统，随时可以使用测定法和生化试剂。
因子IIa（凝血酶）的合成显色底物，高度纯化和稳定。甘露醇作为填充剂。摩尔浓度：45μmol/小瓶。
SKU：5D-30805
分类：发色和荧光底物，质量控制试剂EP和USP
5-CHROM-38凝血酶显色底物，用于根据欧洲药典/美国药典确定LMWH和UFH的抗FIIa活性。
| 尺寸 |
小瓶25毫克 |
|---|
5-diagnostics 5D-51426说明书
5-CLOT NAPTT试剂是一种合成的磷脂血小板替代品，旨在与欧洲药典（EP）方法一起用于检测治疗产品中的活化凝血因子。
SKU：5D-51426 分类：EP＆USP质量控制试剂，测试盒
5-CLOT NAPTT试剂是一种合成的磷脂血小板替代品，旨在与欧洲药典（EP）方法一起用于检测治疗产品中的活化凝血因子。5-CLOT NAPTT试剂也可用于非活化的部分凝血活酶时间（NaPTT）测试中，用于研究人血浆样品中的接触活化和高凝性。不用于诊断过程。
| 尺寸 |
5 x 10毫升 |
|---|
如今，大部分细胞在塑料培养皿的表面生长，也就是说，是二维的。但在体内，细胞则被其他细胞、组织和细胞外基质重重包围。
于是，研究人员开始尝试3D细胞培养，并观察它与2D培养的差异，包括基因表达、信号转导、细胞增殖、细胞形态、细胞间相互作用以及药物反应的改变。
幸运的是，从2D到3D细胞培养系统的转换并非想象中那么困难，而且也是相当值得的。“你的收获在于所研究的细胞更接近它们的真实环境，这对你的生物学研究有好处，”TAP Biosystems的RAFT开发主管Grant Cameron如是说。
下面我们就来介绍一些从2D向3D转换时的注意事项。
3D系统的类型
3D细胞培养基质（或支架）为细胞培养环境增添了第三维。它们有几种主要的类型，包括来自天然材料的水凝胶支架，如细胞外基质胶原蛋白。TAP Biosystems的RAFT（Real Architecture for 3D Tissue）技术正是基于胶原蛋白，它让研究人员能够开展细胞生物学、药物开发和组织工程等研究。
Life Technologies的Geltrex®和BD Biosciences的BD Matrigel™是从小鼠肿瘤中提取出的基底膜基质，Sigma-Aldrich的MaxGel™是人细胞外基质，而Life Technologies的AlgiMatrix来源于褐色海藻。Microtissues公司在其3D 培养皿上使用基于琼脂糖的水凝胶。据介绍，在这种培养皿上，细胞会聚集并自我组装成数百个大小均一的多细胞球体。
中所使用的第二种类型的支架是合成的水凝胶，有时也包括仿生肽，它们可增强整合素的结合。BD Biosciences的BD™ PuraMatrix™肽段水凝胶和Sigma-Aldrich的HydroMatrix™就属于这一类型，而Cellendes的3D Life Biomimetic 水凝胶也是由聚合物和交联剂制成的。
其他类型的人造支架是来自组织培养的塑料，甚至是可生物降解的塑料。还有一个系统，是利用磁悬浮让细胞悬浮，其中细胞产生自己的细胞外环境，而无需塑料支架。
n3D Biosciences公司的Bio-Assembler™系统就是这种，利用磁悬浮来3D培养细胞。该公司的科学家Glauco Souza介绍：“我们利用磁性纳米颗粒来实现，而无需支架。你可以利用磁性笔来移动细胞，创建组织层，然后拿走磁铁，细胞就自由了。”Souza领导的研究小组利用这种方法生成了一种四层的细胞结构，类似于肺组织。
此外，还有一些系统可诱导球体形成，如B-Bridge的Lipidure-Coat板、3D Biomatrix的Perfecta3D®悬滴板，以及STEMCELL的AggreWell™，适合人诱导多能干细胞和胚胎干细胞。
何时转换
越来越多的证据表明，在3D培养系统中生长的细胞会表现出更生理的表型、相互作用及反应。因此，当结果的生理相关性很重要，或者你无法通过其他方式来准确重现细胞行为时，或许应该考虑向3D转换。
AMSBIO的业务部Peter Rettenberger认为：“细胞在平坦的塑料表面上生长，这与它们天然的生理环境相距甚远，也需要一定程度的适应。与之相关的是对细胞的某种选择压力。”
当然，对于了解报告基因表达的转染实验而言，3D培养可能并不重要。但如果你研究的是复杂的药物相互作用，情况就大不相同了。在3D培养中，细胞对抗癌药物的敏感性可能与活体肿瘤更为相似。3D微组织中的药物运输和扩散更像3D的器官和组织。
现在你准备好了？以下这些秘诀可帮助你平稳过渡。首先，注意接种密度，需要比2D培养更高。Cameron解释道：“许多细胞要彼此靠近，甚至要与其他细胞类型共同培养。每孔中的细胞数量应增加，10-20倍是个合理的考虑因素。”他还建议在开展实验之前让细胞有一些过渡时间。
选择的3D支架材料也是成功过渡的一个关键因素。Cardy建议考虑几个关键问题：“我是否需要模拟细胞的天然细胞外基质？是否需要细胞形成有功能的多细胞结构？是否需要通过显微镜来分析我的3D培养，和/或回收细胞用于进一步分析？我是否想要控制凝胶的刚度和生物活性成分？”这些问题的答案可以帮助你缩小选择范围。
小心陷阱
支架的选择也为3D培养的过渡带来陷阱，因为它让一些分析方法复杂化。显微镜观察就变得很有挑战性。2D培养的单层细胞很容易成像，而3D培养物的多层细胞会散射光线。共聚焦显微镜则很好地解决了这个问题，但其他类型的分析也许还很难。Cardy认为，许多支架都不适合免疫组化技术或细胞复苏。
此外，细胞的行为也可能与2D有很大区别，例如，形成更的结构，如癌症肿瘤样。正如Cameron所言：“开辟了一系列新的细胞反应和可能，但这些也会让研究人员大吃一惊。”
columbiabiosciences D3-110说明书
SKU：D3-110
Upon reconstitution, product should be stored at 4°C and used within 8 months.
最大激励∧：
652nm
最大排放∧：
657.5nm
主持人:
山羊
目标：
IgG
反应性：
人类
共轭/标记：
可靠的™ 别藻蓝蛋白
克隆性：
多克隆
同型：
IgG
表格/储存：
冻干，在4°C下储存。
稳定性：
重组后，产品应在4°C下储存，并在8个月内使用。
SKU: D3-1310-100
复溶后，产品应在 4°C 下储存并在 8 个月内使用。
山羊抗 GST pAb 的活性
转染的 HEK293 细胞的流式细胞术分析。用 1 µg/ml 山羊抗 GST:SureLight APC 染色的固定和透化细胞。
阴影直方图 – HEK293 细胞，表达 GST 标签蛋白
开放直方图 – 模拟转染对照 HEK293 细胞
columbiabiosciences D17-1310说明书
SKU: D17-1310
产品应在 2-8°C 下避光储存，并在 6 个月内使用。
美国Epigentek集团公司是先进的表观遗传学相关研究的技术创新者和产品开发供应商。公司开发了700多个产品，为表观遗传学方面的研究和新药研发提供全面系统的解决方案。相比普通方法的产品而言，这些*的产品在科研分析过程中更简单、更快捷、更方便、更有效。*的备受推崇的学者、先进的制药企业以及先进的生物技术公司都在使用Epigentek产品。让客户满意和帮助客户在试验中取得成功是我们进行技术创新的首要任务。
|
目录号 |
尺寸 |
|
A-1018-010 |
10微克 |
|
A-1018-050 |
50微克 |
|
A-1018-100 |
100微克 |
|
应用： |
DB，ELISA，IF，IHC，MeDIP |
|
研究领域： |
DNA甲基化 |
|
克隆： |
单克隆 |
|
共轭： |
非结合 |
|
主办： |
老鼠 |
|
同种型： |
的IgG1 |
|
纯化： |
亲和纯化 |
|
反应： |
范围广泛 |
|
100％保证： |
6个月 |
|
背景 浓度 描述 特异性 同种型 配方 储存 4°C。对于长期储存，等分并储存在-20°C。避免反复冻融。多次冷冻/解冻循环可能导致性能下降。自发货之日起6个月内稳定。 纯度 处理建议 替代名称 应用 |
图1. 含有胞嘧啶（C），5-甲基胞嘧啶（5mC）或5-羟甲基胞嘧啶（5hmC）的DNA寡核苷酸的A-1018的斑点印迹分析。将10ng C，5mC和5hmC DNA寡核苷酸各自点在膜上。将膜与0.5μg/ ml的Epigentek的5hmC克隆HMC / 4D9抗体（1：2000稀释）一起温育，然后与过氧化物酶缀合的山羊抗小鼠IgG（稀释度1：3000）一起温育。仅用5-hmC DNA寡核苷酸观察到特异性信号。 图2. Epigentek的5-hmC克隆HMC / 4D9抗体对5-hmC DNA的高灵敏度和特异性的证明。分别将含有5-hmC，5-mC或仅含有胞嘧啶的合成DNA寡核苷酸以不同浓度添加到测定孔中，然后仅用5-hmC DNA检测特异性信号。
图3. 羟甲基化DNA的选择性富集。将50μg未甲基化，甲基化和羟甲基化的DNA寡核苷酸分别掺入片段化的人基因组DNA（500ng）中。用Epigentek的5-hmC克隆HMC / 4D9抗体处理hMeDIP。用引物通过实时PCR分析洗脱的DNA，以检测掺入的DNA寡核苷酸的存在。倍数富集代表回收的DNA寡核苷酸的量，并且基于Cts计算。 |
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Cat. # |
Antibody Panel Pack |
Modifications |
Sizes Each |
||||
|
C10000 |
Histone H3 Methylation Antibody Panel Pack I- Active Genes |
|
25 µg |
||||
|
C10001 |
Histone H3 Methylation Antibody Panel Pack I – Repression Genes |
|
25 µg |
||||
|
C10002 |
Histone H3 Methylation Antibody Panel Pack II – Active Genes |
|
25 µg |
||||
|
C10003 |
Histone H3 Methylation Antibody Panel Pack II – Repression Genes |
|
25 µg |
||||
|
C10004 |
Histone H3 Methylation Antibody Panel Pack III – Active Genes |
|
25 µg |
||||
|
C10005 |
Histone H3K4 Methylation Antibody Panel Pack |
|
25 µg |
||||
|
C10006 |
Histone H3K9 Methylation Antibody Panel Pack |
|
25 µg |
||||
|
C10007 |
Histone H3K27 Methylation Antibody Panel Pack |
|
25 µg |
||||
|
C10008 |
Histone H3K36 Methylation Antibody Panel Pack |
|
25 µg |
||||
|
C10009 |
Histone H3K79 Methylation Antibody Panel Pack |
|
25 µg |
||||
|
C10010 |
Histone H3 Acetylation Antibody Panel Pack I |
|
25 µg |
||||
|
C10011 |
Histone H3 Acetylation Antibody Panel Pack II |
|
25 µg |
||||
|
C10012 |
Histone H4K20 Methylation Antibody Panel Pack |
|
25 µg |
||||
|
C10013 |
Histone H4 Acetylation Antibody Panel Pack |
|
25 µg |
||||
|
C10014 |
Histone H3 Phosphorylation Antibody Panel Pack |
|
25 µg |
||||
|
C10015 |
Histone H3R2 Methylation Antibody Panel Pack |
|
25 µg |
||||
|
C10016 |
Histone H3R8 Methylation Antibody Panel Pack |
|
25 µg |
||||
|
C10017 |
Histone H3R17 Methylation Antibody Panel Pack |
|
25 µg |
||||
|
C10018 |
Histone H3R26 Methylation Antibody Panel Pack |
|
25 µg |
||||
|
C10019 |
Histone H4R3 Methylation Antibody Panel Pack |
|
25 µg |
|
Image |
Description |
Cat. No. |
Size |
Price |
5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9]
For antibody-based applications requiring high specificity to hydroxymethylated cytosines
Citations (4)
|
Application: |
DB, ELISA, IF, IHC, MeDIP |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Monoclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Mouse |
|
Isotype: |
IgG1 |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Broad Range |
|
A-1018-010 |
10 µg |
|
A-1018-050 |
50 µg |
|
A-1018-100 |
100 µg |
Availability: Usually Ships In 1-2 Days
Antibodies
5-Methylcytosine (5-mC) Monoclonal Antibody [33D3]
For antibody-based applications requiring high specificity to methylated cytosines
Citations (66) | (2)
|
Application: |
DB, ELISA, IF, IHC, IP, MeDIP |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Monoclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Mouse |
|
Isotype: |
IgG1 |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Broad Range |
|
A-1014-010 |
10 µg |
|
A-1014-050 |
50 µg |
|
A-1014-100 |
100 µg |
Availability: Usually Ships in 1 Day or Same Day NY Delivery
Antibodies
ALKBH3 Polyclonal Antibody
|
Application: |
WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Human, Mouse, Rat |
|
A-2723-050 |
50 µg |
|
A-2723-100 |
100 µg |
Availability: Usually Ships in 3-4 Days
Antibodies
ALKBH3 Polyclonal Antibody
|
Application: |
ELISA, IF, IHC |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
Trial Size Available: |
Yes |
|
A57365-020 |
20 µg |
|
A57365-050 |
50 µg |
|
A57365-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
ALKBH3 Polyclonal Antibody, Biotin Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Biotin |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A57368-050 |
50 µg |
|
A57368-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
ALKBH3 Polyclonal Antibody, FITC Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
FITC |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A57367-050 |
50 µg |
|
A57367-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
ALKBH3 Polyclonal Antibody, HRP Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
HRP |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A57366-050 |
50 µg |
|
A57366-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
apaLIM Polyclonal Antibody
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Acetobacter pasteurianus |
|
Trial Size Available: |
Yes |
|
A50869-020 |
20 µg |
|
A50869-050 |
50 µg |
|
A50869-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
apaLIM Polyclonal Antibody, Biotin Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Biotin |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Acetobacter pasteurianus |
|
A50872-050 |
50 µg |
|
A50872-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
apaLIM Polyclonal Antibody, FITC Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
FITC |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Acetobacter pasteurianus |
|
A50871-050 |
50 µg |
|
A50871-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
apaLIM Polyclonal Antibody, HRP Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
HRP |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Acetobacter pasteurianus |
|
A50870-050 |
50 µg |
|
A50870-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
APOBEC3A Polyclonal Antibody
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
Trial Size Available: |
Yes |
|
A58106-020 |
20 µg |
|
A58106-050 |
50 µg |
|
A58106-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
APOBEC3A Polyclonal Antibody, Biotin Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Biotin |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A58107-050 |
50 µg |
|
A58107-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
APOBEC3A Polyclonal Antibody, FITC Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
FITC |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A58108-050 |
50 µg |
|
A58108-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
APOBEC3A Polyclonal Antibody, HRP Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
HRP |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A58109-050 |
50 µg |
|
A58109-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
CGBP Polyclonal Antibody
|
Application: |
ELISA, IF, IP, WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Human, Mouse, Rat |
|
A-1013-050 |
50 µg |
|
A-1013-100 |
100 µg |
Availability: Usually Ships In 1-2 Days
Antibodies
CGGBP1 Polyclonal Antibody
|
Application: |
ELISA, IHC |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
Trial Size Available: |
Yes |
|
A68018-020 |
20 µg |
|
A68018-050 |
50 µg |
|
A68018-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
CGGBP1 Polyclonal Antibody, Biotin Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Biotin |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A68021-050 |
50 µg |
|
A68021-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
CGGBP1 Polyclonal Antibody, FITC Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
FITC |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A68020-050 |
50 µg |
|
A68020-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
CGGBP1 Polyclonal Antibody, HRP Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
HRP |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A68019-050 |
50 µg |
|
A68019-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
DNMT1 Monoclonal Antibody [60B1220.1]
Citations (7)
|
Application: |
ELISA, IF, IP, WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Monoclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Mouse |
|
Isotype: |
IgG1 |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Human, Mouse |
|
A-1001-050 |
50 µg |
|
A-1001-100 |
100 µg |
Availability: Usually Ships in 1 Day or Same Day NY Delivery
Antibodies
DNMT1 Polyclonal Antibody
Citations (2)
|
Application: |
ChIP, IF, IHC, IP, WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Human, Mouse, Rat |
|
A-1700-050 |
50 µg |
|
A-1700-100 |
100 µg |
Availability: Usually Ships in 3-4 Days
Antibodies
DNMT2 Polyclonal Antibody
|
Application: |
IHC, WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Human, Mouse |
|
A-1002-050 |
50 µl |
|
A-1002-100 |
100 µl |
Availability: Usually Ships in 3-4 Days
Antibodies
DNMT3A Polyclonal Antibody
Citations (4)
|
Application: |
IF, IHC, WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Human, Mouse, Rat |
|
A-1003-050 |
50 µg |
|
A-1003-100 |
100 µg |
Availability: Usually Ships in 3-4 Days
Antibodies
DNMT3A Polyclonal Antibody
|
Application: |
ELISA, IF, IHC |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human, Mouse |
|
Trial Size Available: |
Yes |
|
A52569-020 |
20 µg |
|
A52569-050 |
50 µg |
|
A52569-100 |
100 µg |
Availability: Usually Ships Within 1 Week
Antibodies
DNMT3A Polyclonal Antibody, Biotin Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Biotin |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A52572-050 |
50 µg |
|
A52572-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
DNMT3A Polyclonal Antibody, FITC Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
FITC |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A52571-050 |
50 µg |
|
A52571-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
DNMT3A Polyclonal Antibody, HRP Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
HRP |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A52570-050 |
50 µg |
|
A52570-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
DNMT3B Polyclonal Antibody
Citations (5)
|
Application: |
WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Human, Mouse |
|
A-1004-050 |
50 µg |
|
A-1004-100 |
100 µg |
Availability: Usually Ships In 1-2 Days
Antibodies
DNMT3L Monoclonal Antibody [B0-5]
|
Application: |
ICC, IHC |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Monoclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Mouse |
|
Isotype: |
IgG1 |
|
Purification: |
Protein G Purified |
|
Reactivity: |
Human, Mouse |
|
A-1800-100 |
100 µl |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
DNMT3L Polyclonal Antibody
|
Application: |
IF, IP, WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Human |
|
A-1005-050 |
50 µg |
|
A-1005-100 |
100 µg |
Availability: Usually Ships in 3-4 Days
Antibodies
ecoRVM Polyclonal Antibody
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Escherichia coli |
|
Trial Size Available: |
Yes |
|
A57405-020 |
20 µg |
|
A57405-050 |
50 µg |
|
A57405-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
ecoRVM Polyclonal Antibody, Biotin Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Biotin |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Escherichia coli |
|
A57408-050 |
50 µg |
|
A57408-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
ecoRVM Polyclonal Antibody, FITC Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
FITC |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Escherichia coli |
|
A57407-050 |
50 µg |
|
A57407-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
ecoRVM Polyclonal Antibody, HRP Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
HRP |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Escherichia coli |
|
A57406-050 |
50 µg |
|
A57406-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
GATAD2A Polyclonal Antibody
|
Application: |
ELISA, IHC, WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human, Rat |
|
Trial Size Available: |
Yes |
|
A69019-020 |
20 µg |
|
A69019-050 |
50 µg |
|
A69019-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
GATAD2A Polyclonal Antibody, Biotin Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Biotin |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A69022-050 |
50 µg |
|
A69022-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
GATAD2A Polyclonal Antibody, FITC Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
FITC |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A69021-050 |
50 µg |
|
A69021-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
GATAD2A Polyclonal Antibody, HRP Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
HRP |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A69020-050 |
50 µg |
|
A69020-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
HINFP Polyclonal Antibody
|
Application: |
ELISA, WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human, Rat |
|
Trial Size Available: |
Yes |
|
A69539-020 |
20 µg |
|
A69539-050 |
50 µg |
|
A69539-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
HINFP Polyclonal Antibody, Biotin Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Biotin |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A69542-050 |
50 µg |
|
A69542-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
HINFP Polyclonal Antibody, FITC Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
FITC |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A69541-050 |
50 µg |
|
A69541-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
HINFP Polyclonal Antibody, HRP Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
HRP |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A69540-050 |
50 µg |
|
A69540-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
MBD1 Polyclonal Antibody
Citations (3)
|
Application: |
E, IF, IP, WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Human, Mouse, Rat |
|
A-1006-050 |
50 µg |
|
A-1006-100 |
100 µg |
Availability: Usually Ships In 1-2 Days
Antibodies
MBD2 Polyclonal Antibody
|
Application: |
WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Mouse |
|
A-1713-050 |
50 µg |
|
A-1713-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
MBD3 Polyclonal Antibody
|
Application: |
WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Human |
|
A-1008-050 |
50 µg |
|
A-1008-100 |
100 µg |
Availability: Usually Ships in 3-4 Days
Antibodies
MBD5 Polyclonal Antibody
|
Application: |
ELISA, WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Human, Mouse, Rat |
|
A-3729-050 |
50 µl |
|
A-3729-100 |
150 µl |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
MBD5 Polyclonal Antibody
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
Trial Size Available: |
Yes |
|
A67422-020 |
20 µg |
|
A67422-050 |
50 µg |
|
A67422-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
MBD5 Polyclonal Antibody, Biotin Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Biotin |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A67425-050 |
50 µg |
|
A67425-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
MBD5 Polyclonal Antibody, FITC Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
FITC |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A67424-050 |
50 µg |
|
A67424-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
MBD5 Polyclonal Antibody, HRP Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
HRP |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A67423-050 |
50 µg |
|
A67423-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
MeCP2 Polyclonal Antibody
|
Application: |
IF, IHC, WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Human, Mouse, Rat |
|
A-1012-050 |
50 µg |
|
A-1012-100 |
100 µg |
Availability: Usually Ships In 1-2 Days
Antibodies
N6AMT2 Polyclonal Antibody
|
Application: |
ELISA, WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
Trial Size Available: |
Yes |
|
A51061-020 |
20 µg |
|
A51061-050 |
50 µg |
|
A51061-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
N6AMT2 Polyclonal Antibody, Biotin Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Biotin |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A51064-050 |
50 µg |
|
A51064-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
N6AMT2 Polyclonal Antibody, FITC Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
FITC |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A51063-050 |
50 µg |
|
A51063-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
N6AMT2 Polyclonal Antibody, HRP Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
HRP |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A51062-050 |
50 µg |
|
A51062-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
sacIM Polyclonal Antibody
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Streptomyces achromogenes |
|
Trial Size Available: |
Yes |
|
A57469-020 |
20 µg |
|
A57469-050 |
50 µg |
|
A57469-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
sacIM Polyclonal Antibody, Biotin Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Biotin |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Streptomyces achromogenes |
|
A57472-050 |
50 µg |
|
A57472-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
sacIM Polyclonal Antibody, FITC Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
FITC |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Streptomyces achromogenes |
|
A57471-050 |
50 µg |
|
A57471-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
sacIM Polyclonal Antibody, HRP Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
HRP |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Streptomyces achromogenes |
|
A57470-050 |
50 µg |
|
A57470-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
scaIM Polyclonal Antibody
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Streptomyces caespitosus |
|
Trial Size Available: |
Yes |
|
A56851-020 |
20 µg |
|
A56851-050 |
50 µg |
|
A56851-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
scaIM Polyclonal Antibody, Biotin Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Biotin |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Streptomyces caespitosus |
|
A56854-050 |
50 µg |
|
A56854-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
scaIM Polyclonal Antibody, FITC Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
FITC |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Streptomyces caespitosus |
|
A56853-050 |
50 µg |
|
A56853-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
scaIM Polyclonal Antibody, HRP Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
HRP |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Streptomyces caespitosus |
|
A56852-050 |
50 µg |
|
A56852-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
TET1 Polyclonal Antibody
Citations (1)
|
Application: |
IP, WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Human, Mouse, Rat |
|
A-1020-050 |
50 µg |
|
A-1020-100 |
100 µg |
Availability: Usually Ships in 3-4 Days
Antibodies
TET2 Polyclonal Antibody
|
Application: |
IHC, WB |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
Affinity Purified |
|
Reactivity: |
Human, Mouse, Rat |
|
A-1701-050 |
50 µg |
|
A-1701-100 |
100 µg |
Availability: Usually Ships in 3-4 Days
Antibodies
TET3 Polyclonal Antibody
|
Application: |
ELISA, IHC |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Unconjugated |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
Trial Size Available: |
Yes |
|
A50520-020 |
20 µg |
|
A50520-050 |
50 µg |
|
A50520-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
TET3 Polyclonal Antibody, Biotin Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
Biotin |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A50523-050 |
50 µg |
|
A50523-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
TET3 Polyclonal Antibody, FITC Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
FITC |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A50522-050 |
50 µg |
|
A50522-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
Antibodies
TET3 Polyclonal Antibody, HRP Conjugated
|
Application: |
ELISA |
|
Areas of Research: |
DNA Methylation |
|
Clonality: |
Polyclonal |
|
Conjugate: |
HRP |
|
Host: |
Rabbit |
|
Isotype: |
IgG |
|
Purification: |
>95%, Protein G Purified |
|
Reactivity: |
Human |
|
A50521-050 |
50 µg |
|
A50521-100 |
100 µg |
Availability: Usually Ships Within 1-2 Weeks
上海金畔生物科技有限公司是实验试剂一站式采购服务商
1：强大的进口辐射能力，血清、抗体、耗材、大部分限制进口品等。
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3：提供加急服务，货品一般1-2周到货。
4：富有竞争力的价格优势，绝大部分价格有优势。
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6：我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等几十家国外公司代理。
7：我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发，欢迎合作。
ChemBridge过去二十多年一直是高质量筛选化合物和文库的提供者，并根据行业和学术界对药物发现和化学生物学研究人员不断发展的要求，不断生产新颖的，类药物和类似铅的筛选化合物。
chembridge 10500388说明书
1- -4-吡啶-4-基-1,4-二氮杂-6-醇
显示类似物：2D | 3D | 2D＆3D
式
C 18 H 21 N 5 O 2
分子量
339
对数
0.82
日志软件
-1.37
可旋转债券
4
don
2
哈卡
5
tPSA
81.4
形成
干膜
hit2lead价格组
4
| 量 | 数量 |
| 1毫克 | |
| 5微摩尔 |
上海金畔售后保障
正品保证
上海金畔所有产品都是自运营的，我们已经跟国外多家厂方建立品牌推广合作关系,获得对方的支持和；确保货物的来源； 正规报关，提供13%增值税发票。
及时交付
上海金畔的运营团队都是有着多年经验的成员，他们熟悉海外采购、仓储物流、报关等环节；上海金畔的进口速度比传统企业提高了50%以上， 部分产品甚至能做到7-10天到货。
轻松采购
上海金畔的价格是真实透明的，并且具有很大的价格优势，不需要繁杂的询价比价；
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上海金畔提供的产品在使用过程中如因产品质量问题有售后需求时， 上海金畔产品顾问会及时为您处理。
TheWell Bioscience成立于2016年，是一家非常新的公司，公司专注于3D细胞培养基的开发与研究，其开发出的VitroGel 3D 是一种无动物源性多糖水凝胶体系，
上海金畔生物科技有限公司作为TheWell Bioscience中国*代理，为您的3D细胞培养带来不一样的体验
VitroGel 3D具有如下优点：
1：操作方便：室温下就可以操作，简单混合即可
2：省时间 ：只要15分钟就可以凝结，大大缩短了实验时间
3：水凝胶是透明的，与不同的成像系统相容，便于细胞观察。
4：氧气，营养和其他分子可以轻松地进出水凝胶系统，更适合药物研发！
5：更容易就可以收集培养细胞—只需要简单的离心就可以了
6: 水凝胶尽然还可以直接用于注射—大大方便了体内研究
2017年4月，科学出版了“ 增加细胞培养深度 ”的技术专题文章，讨论了3D细胞培养的发展。本文介绍了水凝胶，微载体，悬滴板和超低附着孔板等现有技术的优缺点。来自TheWell Bioscience的即用型水凝胶系统的VitroGel 3D已被强调为3D细胞培养及其以外的更易于使用的解决方案。
诱导多能干细胞（iPSCs）的3D培养（细胞起始数：2.5×10 5个细胞/ mL;产品：VitroGel 3D-RGD; 时间：7天）
Beta Lox 5（BL5）细胞的3D培养（细胞起始数：2.5×10 5个细胞/ mL; 产品：VitroGel 3D; 时间：7天）
人类淋巴母细胞普列克细胞的二维涂层 （细胞起始数：：2.5×10 5个细胞/ mL;产品：VitroGel 3D; 时间：7天）
1：VitroGel 3D可以简单地与细胞混合用于3D细胞培养。水凝胶基质支持细胞随着时间的推移生长为3D菌落。可以加入药物或其他化合物以渗透到水凝胶体系中进行筛选
2：VitroGel 3D可以用于涂抹细胞生长的物质。物质的不同刚度和质地可能会导致不同的细胞行为。此外，随着时间的推移，细胞可能进入入侵/迁移研究的水凝胶基质。
3：通过以适当的比例（推荐4：1 v / v）混合hdyrogel溶液和细胞悬浮液（或其他离子溶液），可以形成可注射的水凝胶，用于异种移植，药物递送，生物印刷，微阵列等。
另外，可以对不同的物理/化学特性和凝胶形成时间操纵水凝胶性质和制备方案。水凝胶溶液的浓度也可以根据不同的水凝胶强度进行调整。可以通过这些灵活性来实现多种应用。
Thewellbio应用3D培养技术可以实现体外研究细胞在体内的行为与对刺激的反应，如温度、pH变化、营养吸收、转运及分化等。公司研发供应的细胞体外3D培养体系采用无动物源成分的多糖凝胶材料，模拟出天然的细胞胞外基质环境，同时具有多用途、高精度、高性价比、操作简便和节省时间等特点。因此3D培养技术越来越多地被应用到药物筛选、组织工程、临床前研究、细胞治疗和基础研究等领域。
1.我应该选择哪种产品，VitroGel 3D或VitroGel 3D-RGD？
如果您使用贴壁细胞系，建议您使用VitroGel 3D-RGD。如果您使用非贴壁细胞系，或者您想要更灵活地控制生长因子/蛋白质，我们建议您使用VitroGel 3D。请support@thewellbio.com如果你需要一个定制的产品。
2.产品有多稳定？
VitroGel 3D在4-40°C之间是稳定的。请勿将产品冷冻或将水凝胶溶液加热至80°C以上。该产品在室温下4℃或6个月未开封未开封18个月。一旦打开，请密封盖子，保持在4°C，以防止1个月内污染和使用。
3.如何调整水凝胶形成时间并作为可注射水凝胶进行准备？
水凝胶形成时间高度取决于水凝胶溶液和触发溶液（细胞培养基/PBS）的混合比例。我们建议将水凝胶溶液和介质/ PBS以4：1（v / v）的比例混合成可注射的水凝胶。水凝胶在混合后30分钟内应稳定。较高的混合比（水凝胶溶液：介质> 4：1）会导致更长的水凝胶形成时间。低混合比（水凝胶溶液：介质<1：1）可能导致不可注射的水凝胶。
4.水凝胶形成速度太快，我该怎么办？
水凝胶形成时间取决于几个因素，如凝胶浓度，培养基的离子强度和混合比。如果水凝胶形成太快，我们建议用DI水稀释水凝胶溶液，然后与细胞培养基混合使其凝胶化。稀释后，尝试保持4：1的比例（稀释的水凝胶溶液：细胞培养基= 4：1）进行混合，因为这是我们用于我们的水凝胶系统的大多数培养基的*比例。
5.当将凝胶溶液与细胞培养基混合时，我可以做什么来保持气泡形成？
气泡问题与将凝胶溶液与细胞培养基混合后溶液粘度增加有关。这里有一些有助于减少气泡形成的建议：
1）将水凝胶溶液预热至37°C以降低凝胶溶液的粘度。
2）将凝胶溶液和细胞培养基轻轻混合，并沿着孔板的壁缓慢移液，不引入气泡。（有些用户喜欢使用涡流而不是移液管进行混合以避免气泡）
3）在与细胞培养基混合之前用DI水稀释凝胶溶液。
4）切割移液器吸头以获得更好的流量。
5）如果混合后仅形成小气泡，在水凝胶顶部加入一层培养基，孵育过夜。
6.如何调整zui终水凝胶的硬度？
通过在与细胞培养基/PBS混合之前稀释水凝胶溶液可以调节zui终水凝胶的硬度。请使用无菌去离子水稀释水凝胶溶液。不要使用PBS或其他离子溶液。如果您需要比原始产品更高的水凝胶刚度，请与我们。
7.应该使用什么种子密度？
我们建议对该水凝胶系统使用200,000-1,000,000个细胞/ mL。您可能需要相应地每1-2天更换一次覆盖的培养基。
8.细胞在VitroGel 3D水凝胶中生长多长时间？
我们已经测试了水凝胶系统中细胞的生长长达两周。依靠细胞系，3D细胞培养可以持续更长时间。一旦单元格的数量和大小增加，您可能需要更频繁地更换封面介质。
10.球形形成前多久？
通常，球形体形成在水凝胶体系中培养约5-10天。形成时间可以根据细胞系而变化。
11.3D文化后，我能从水凝胶中收获细胞么？
是的，通过使用简单的移液和离心方案，可以在3D培养后收获细胞。请下载完整的使用手册，并阅读从水凝胶收集细胞的细节。
12.为什么水凝胶松散地粘到未处理的组织培养板上？
未处理的组织培养板具有更疏水的表面，其减少了孔板表面上的水凝胶/细胞的附着。为了更好的表现，我们建议使用经过处理的组织培养板与VitroGel 3D系统。
13.胶凝是否可逆？
是。如果水凝胶以适当的混合比制备，例如 4：1（水凝胶溶液：细胞培养基= 4：1），水凝胶将进行特殊的可逆机械性能 – 剪切稀化和自愈，这意味着水凝胶可以在剪切力下转移到液体中，例如移液或一旦剪切力停止，再次注入和恢复为水凝胶。除了这种机制，我们还可以操纵水凝胶对温度变化或其他方法是可逆的。请support@thewellbio.com获取定制解决方案。
14.在VitroGel 3D中生长的细胞是否可以分化？
是。通过使用新鲜的VitroGel 3D或其他2D或3D培养方法，可以从VitroGel 3D水凝胶系统和亚培养物中再次收获细胞一段时间。
15如何使用VitroGel 3D进行共培养或夹心培养（逐层）？
可以使用VitroGel 3D水凝胶系统进行两种或多种不同细胞类型的共培养。除了添加不同的细胞类型与水凝胶溶液共同培养，每种细胞类型也可以与水凝胶溶液混合并逐层加入。在*层水凝胶变得稳定后，仔细地将第二层细胞/水凝胶混合物覆盖在*层细胞/水凝胶的顶部。
16.我可以将细胞外基质蛋白或其他分子化合物添加到VitroGel 3D水凝胶中吗？
是。细胞外基质蛋白或其他分子化合物可以在水凝胶形成之前或之后加入到VitroGel 3D水凝胶体系中。在水凝胶形成之前，将蛋白质或分子化合物加入细胞培养基中，然后与VitroGel 3D水凝胶溶液混合。在形成水凝胶之后，可以将蛋白质或分子化合物加入渗透到纳米纤维基质中的水凝胶的顶部。请注意，由于蛋白质或化学化合物中含有的盐，水凝胶形成时间和zui终凝胶硬度可能会发生变化。如果改变水凝胶性质，我们建议在与VitroGel 3D水凝胶溶液混合之前用蔗糖溶液洗涤蛋白质或化合物以除去盐。
17.VitroGel 3D是否与共焦显微镜或其他成像技术的染色和免疫荧光方案相兼容？
是。细胞可以在水凝胶内染色或从水凝胶中收获，然后染色。大多数荧光染料和免疫试剂可以用于标准方案。请阅读VitroGel 3D的完整使用手册了解更多详情。此外，VitroGel 3D水凝胶在不同的成像系统中是透明和兼容的，用于细胞观察。
18.我可以对在VitroGel 3D中培养的细胞的蛋白质和核酸进行分子分析吗？
是。在VitroGel 3D水凝胶中培养后，可以从水凝胶中收获细胞，并根据标准程序进行分子分析。
19.水凝胶是否可以从组织培养中撤出，以进行切片？
是的，一旦水凝胶变得稳定，你可以把它从文化中分离出来。添加*培养基后30-60分钟内水凝胶应稳定。
20.我可以使用VitroGel 3D进行体内研究吗？
是。可以在水凝胶形成之前或之后注射VitroGel 3D用于体内研究。在水凝胶形成之前，可以将VitroGel 3D溶液直接注射到动物中，后者随着其与生理环境的离子化合物接触而变成水凝胶。此外，VitroGel 3D水凝胶在水凝胶形成后具有先进的注射性能。以适当的比例混合水凝胶溶液和细胞培养基/PBS（我们推荐水凝胶溶液：培养基/PBS = 4:1v / v），zui终的水凝胶变得可注射用于体内研究。使用这种方法，细胞或其他化合物可以在注射前在水凝胶中很好地混合。
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