Emfret D200说明书
产品详情
Cat.No.:XX D200
抗原： 小鼠血小板活化的双色分析
描述： 该产品提供了一种即用型工具，用于通过流式细胞术确定小鼠血小板的整联蛋白αIIbβ3（GPIIb / IIIa）活化和α-颗粒分泌（P-选择蛋白表达）。
克隆： Wug.E9 JON / A.
同种型： 大鼠IgG2b / IgG1
形成： FITC PE
应用： 流式细胞术
尺寸： 2 x 1.0毫升; 200次测试
价钱： 505.00美元
Two-color analysis of mouse platelet activation PE- and FITC- labeled antibodies
小鼠血小板活化PE和FITC标记抗体的双色分析
# D200
克隆/亚型：Jon/A/大鼠(Wistar)IgG2b(1ml-200试验)
内容：Pe-和FITC标记的免疫球蛋白在20 mm Tris缓冲液中加入137 mm NaCl、0.5%BSA和0.09%(w/v)Sodium azide。
大小：2×1.0ml/200试验仅供研究使用，不用于诊断或治疗用途。这种产品不是医疗器械。
该产品提供了一个现成的工具，以确定整合素aIIbb3(GPIIb/IIIa)的激活和无粒细胞分泌(P-选择素表达)在一次分析中小鼠血小板。
特异性：PE标记的jon/A抗体(emfret#m023-2)选择性地结合小鼠整合素aIIb 3 1(GPIIb/IIIa，CD 41/CD 61)的高亲和力构象。 f 135 kDa AIIB链和90 kDa b3链.整合素aIIbb3是纤维蛋白原、血管性假血友病因子、纤维连接蛋白和维甲酸的血小板受体，介导血小板粘附和凝血功能。 环境2。整合素aIIbb3的激活依赖构象变化，因此Jon/APE的结合依赖于细胞外游离钙。
FITC标记的戊型E9抗体(emfret#M130-1)与小鼠P-选择素(CD62P)反应，是一种属于粘附分子3选择素家族的140 kDa单链多肽。P-选择素 在血小板的a-颗粒和内皮细胞的Weibel-Palade小体中表达。细胞活化后，P-选择素被转运到表面膜上.P-选择素介导粘附 活化血小板对中性粒细胞和单核细胞的影响。血小板表面P-选择素的表达是A颗粒分泌的标志.
制备和保存：用蛋白G-Sepharose层析法从杂交瘤细胞培养上清液中纯化抗体.抗体与FITC或R-生理红蛋白(PE)un结合。 在宜条件下。这些解决方案没有未结合的荧光团。将产品保存在4°C未稀释，避免长时间暴露在光线下。从装运之日起稳定一年。别冻着。 在使用前，请将同等体积的A抗体(Jona-PE)和B抗体(WUg.E9-FITC)混合在混合管中。这种混合物可以在4℃下储存几个星期。
用途：抗体的制备是为流式细胞术的应用而优化的。建议用抗体A和B混合液中的10μl对~(106)血小板或~0.5×106细胞进行染色。 25升泰罗德-Hepes缓冲液含1mm CaCl 2的体积。激动剂应与染色抗体一起加入。室温下孵育15分钟，加入400毫升，停止反应。 在30分钟内对样品进行PBS分析。请注意，孵育时间、缓冲液条件或抗体浓度的变化可能会影响结果的质量。
警告：Sodium azide是一种可逆的氧化代谢抑制剂；因此，含有这种防腐剂的抗体制剂不得用于细胞培养或动物体内注射。 。Sodium azide可通过洗涤染色细胞或平板结合抗体或在无Sodium azide缓冲液中透析可溶性抗体来去除。
10 M腺苷对25例胎肝缓冲液(1mm CaCl 2)小鼠血小板106枚活化整合素aIIb 3和P-选择素表达的检测 二磷酸(ADP)或0.2U/ml凝血酶，用10μl A抗体和B抗体直接染色15 min。样品用400 lPBS填充，直接分析。普拉蒂莱 TS采用FSC/SSC特性。注：ADP是一种相对较弱的激动剂，它刺激(可逆)整合素aIIbb3的激活，但实际上没有P-选择素的表达。相反，凝血酶 是一种*的血小板激动剂，可诱导强而不可逆的整合素aIIb 3活化和P-选择素的表达。
References: 1. Bergmeier W, Schulte V, Brockhoff G, Bier U, Zirngibl H, Nieswandt B. (2002) Flow cytometric detection of activated mouse integrin alphaIIbbeta3 with a novel monoclonal antibody. Cytometry 1;48:80-6. 2. Phillips DR, Charo IF, Scarborough RM. (1990) GPIIb-IIIa: the responsive integrin. Cell. 65(3):359-62. 3. Bird MI, Foster MR, Priest R, et al. (1997) Selectins: physiological and pathophysiological roles. Biochem Soc Trans. 25(4):1199-206 4. Bouchard BA, Tracy PB. (2001) Platelets, leukocytes, and coagulation. Curr Opin Hematol. 8(5):263-9
emfret书：
EMFRET Analytics—专注脉管系统抗体
德国EMFRET公司专注心血管和血液系统研究用抗体研发和生产，供应的抗体包括清除血液中血小板、流式细胞术鉴定分析小鼠血小板表面糖蛋白，EMFRET公司的抗体不仅适用于流失分析、WB检测，还可用于免疫组化/免疫荧光及免疫共沉淀检测。
Cat.No. Antigen Clone Isotype Form Applications Size
C301 Antibodies for Mouse Plaet Depletion polyclonal non-immune rat immunoglobulins Rat IgG purif. negative control for #R300 0.5 mg
D200 Two-Color Analysis of Mouse Plaet Activation Wug.E9 JON/A Rat IgG2b/IgG1 FITC PE flow cytometry 2 x 1.0 ml; 200 tests
M011-0 GPVI JAQ1 Rat IgG2a purif. IP, IHC, WB 0.1 mg
M011-1 GPVI JAQ1 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests
M021-0 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.H4 Rat IgG2b purif. IP, IHC, block 0.5 mg
M021-1 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.H4 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests
M022-0 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.A1 Rat IgG1 purif. IP, IHC 0.5 mg
M023-2 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) JON/A Rat IgG2b PE FC (activ. form) 1.5 ml; 300 tests
M024-0 Integrin alphaIIb (GPIIb, CD41) MwReg30 Rat IgG1 purif. IP, IHC, generation of Fab fragments 6 mg
M025-0 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a purif. IP, IHC 0.5 mg
M025-1 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests
M025-2 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a PE FC, IHC 1.5 ml; 300 tests
M025-3 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a DyLight 649 FC, IHC 1.5 ml; 300 tests
M030-0 Integrin beta3 chain (GPIIIa, CD61) Luc.A5 Rat IgG2a purif. IP, IHC, WB 0.5 mg
M031-0 Integrin beta3 chain (GPIIIa, CD61) Luc.H11 Rat IgG2b purif. IP, IHC, WB 0.5 mg
M031-1 Integrin beta3 chain (GPIIIa, CD61) Luc.H11 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests
M040-0 GPIbalpha (CD42b) Xia.G5 Rat IgG2b purif. IP, IHC 0.5 mg
M040-1 GPIbalpha (CD42b) Xia.G5 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests
M040-2 GPIbalpha (CD42b) Xia.G5 Rat IgG2b PE FC, IHC 1.5 ml; 300 tests
M040-3 GPIbalpha (CD42b) Xia.G5 Rat IgG2b DyLight 649 FC, IHC 1.5 ml; 300 tests
M041-0 GPIbalpha (CD42b) Xia.H10 Rat IgG2a purif. FC, IHC 0.5 mg
M041-1 GPIbalpha (CD42b) Xia.H10 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests
M042-0 GPIbalpha (CD42b) Xia.G7 Rat IgG1 purif. IP, IHC, WB 0.5 mg
M042-1 GPIbalpha (CD42b) Xia.G7 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests
M043-0 GPIbalpha (CD42b) Xia.B2 Rat IgG2a purif. IP, IHC, block 0.5 mg
M043-1 GPIbalpha (CD42b) Xia.B2 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests
M050-0 GPIbbeta (CD42c) Xia.C3 Rat IgG2a purif. IP, IHC, WB 0.5 mg
M050-1 GPIbbeta (CD42c) Xia.C3 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests
M051-0 GPIX (CD42a) Xia.B4 Rat IgG1 purif. IP, IHC 0.5 mg
M051-1 GPIX (CD42a) Xia.B4 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests
M060-0 GPV (CD42d) Gon.C2 Rat IgG1 purif. IP, IHC 0.5 mg
M060-1 GPV (CD42d) Gon.C2 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests
M061-1 GPV (CD42d) Gon.G6 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests
M070-0 Integrin alpha2 chain (CD49b, GPIa) Sam.G4 Rat IgG2b purif. IP, IHC, block 0.5 mg
M070-1 Integrin alpha2 chain (CD49b, GPIa) Sam.G4 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests
M071-0 Integrin alpha2 chain (CD49b, GPIa) Sam.C1 Rat IgG2b purif. IP, IHC 0.5 mg
M071-1 Integrin alpha2 chain (CD49b, GPIa) Sam.C1 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests
M075-0 Integrin alpha2 chain (CD49b, GPIa) Sam.C1, Sam.G4, Sam.H9 Rat IgG2b/IgG1 purif. IP, IHC 0.5 mg
M080-0 Integrin alpha5 chain (CD49e) Tap.A12 Rat IgG1 purif. IP, IHC 0.5 mg
M080-1 Integrin alpha5 chain (CD49e) Tap.A12 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests
M110-0 CD9 Nyn.H3 Rat IgG2a purif. IP, IHC, WB 0.5 mg
M110-1 CD9 Nyn.H3 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests
M120-0 CD31 (PECAM-1) Pec.H3 Rat IgG1 purif. IP, IHC, WB 0.5 mg
M130-0 CD62P (P-selectin) Wug.E9 Rat IgG1 purif. IP, IHC 0.5 mg
M130-1 CD62P (P-selectin) Wug.E9 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests
M130-2 CD62P (P-selectin) Wug.E9 Rat IgG1 PE FC, IHC 1.5 ml; 300 tests
P140-1 fibrinogen polyclonal Rabbit IgG FITC FC, IHC 1.5 ml; 300 tests
P150-1 von Willebrand Factor (vWF) polyclonal Rabbit IgG FITC FC, IHC 1.5 ml; 300 tests
P180-1 negative control polyclonal Rabbit IgG FITC FC, IHC 1.0 ml; 200 tests
P190-1 negative control polyclonal Rat IgG FITC FC, IHC 1.0 ml; 200 tests
P190-2 negative control polyclonal Rat IgG PE FC, IHC 1.0 ml; 200 tests
P190-3 negative control polyclonal Rat IgG DyLight 649 FC, IHC 1.0 ml; 200 tests
R300 Antibodies for Mouse Plaet Depletion polyclonal anti-GPIb alpha Rat IgG purif. plaet depletion 0.5 mg
X488 Antibodies for In Vivo Mouse Plaet Labeling anti – GPIbbeta derivative Rat IgG DyLight 488 in vivo 100 µg
X649 Antibodies for In Vivo Mouse Plaet Labeling anti – GPIbbeta derivative Rat IgG DyLight 649 in vivo 100 µg
货号 抗原类型 克隆方式 表型 产品形式 应用 规格
C301 清除小鼠血小板 polyclonal non-immune rat immunoglobulins Rat IgG 纯化 清除小鼠血小板 0.5 mg
D200 双色分析血小板活性 Wug.E9 JON/A Rat IgG2b/IgG1 FITC、PE 流式细胞术 2 x 1.0 ml; 200 tests
M011-0 GPVI JAQ1 Rat IgG2a 纯化 IP, IHC, WB 0.1 mg
M011-1 GPVI JAQ1 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests
M021-0 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.H4 Rat IgG2b purif. IP, IHC, block 0.5 mg
M021-1 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.H4 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests
M022-0 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.A1 Rat IgG1 purif. IP, IHC 0.5 mg
M023-2 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) JON/A Rat IgG2b PE FC (activ. form) 1.5 ml; 300 tests
M024-0 Integrin alphaIIb (GPIIb, CD41) MwReg30 Rat IgG1 purif. IP, IHC, generation of Fab fragments 6 mg
M025-0 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a purif. IP, IHC 0.5 mg
M025-1 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests
M025-2 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a PE FC, IHC 1.5 ml; 300 tests
M025-3 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a DyLight 649 FC, IHC 1.5 ml; 300 tests
M030-0 Integrin beta3 chain (GPIIIa, CD61) Luc.A5 Rat IgG2a purif. IP, IHC, WB 0.5 mg
M031-0 Integrin beta3 chain (GPIIIa, CD61) Luc.H11 Rat IgG2b purif. IP, IHC, WB 0.5 mg
M031-1 Integrin beta3 chain (GPIIIa, CD61) Luc.H11 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests
M040-0 GPIbalpha (CD42b) Xia.G5 Rat IgG2b purif. IP, IHC 0.5 mg
M040-1 GPIbalpha (CD42b) Xia.G5 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests
M040-2 GPIbalpha (CD42b) Xia.G5 Rat IgG2b PE FC, IHC 1.5 ml; 300 tests
M040-3 GPIbalpha (CD42b) Xia.G5 Rat IgG2b DyLight 649 FC, IHC 1.5 ml; 300 tests
M041-0 GPIbalpha (CD42b) Xia.H10 Rat IgG2a purif. FC, IHC 0.5 mg
M041-1 GPIbalpha (CD42b) Xia.H10 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests
M042-0 GPIbalpha (CD42b) Xia.G7 Rat IgG1 purif. IP, IHC, WB 0.5 mg
M042-1 GPIbalpha (CD42b) Xia.G7 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests
M043-0 GPIbalpha (CD42b) Xia.B2 Rat IgG2a purif. IP, IHC, block 0.5 mg
M043-1 GPIbalpha (CD42b) Xia.B2 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests
M050-0 GPIbbeta (CD42c) Xia.C3 Rat IgG2a purif. IP, IHC, WB 0.5 mg
M050-1 GPIbbeta (CD42c) Xia.C3 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests
M051-0 GPIX (CD42a) Xia.B4 Rat IgG1 purif. IP, IHC 0.5 mg
M051-1 GPIX (CD42a) Xia.B4 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests
M060-1 GPV (CD42d) Gon.C2 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests
M061-1 GPV (CD42d) Gon.G6 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests
M070-0 Integrin alpha2 chain (CD49b, GPIa) Sam.G4 Rat IgG2b purif. IP, IHC, block 0.5 mg
M070-1 Integrin alpha2 chain (CD49b, GPIa) Sam.G4 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests
M071-0 Integrin alpha2 chain (CD49b, GPIa) Sam.C1 Rat IgG2b purif. IP, IHC 0.5 mg
M071-1 Integrin alpha2 chain (CD49b, GPIa) Sam.C1 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests
M075-0 Integrin alpha2 chain (CD49b, GPIa) Sam.C1, Sam.G4, Sam.H9 Rat IgG2b/IgG1 purif. IP, IHC 0.5 mg
M080-0 Integrin alpha5 chain (CD49e) Tap.A12 Rat IgG1 purif. IP, IHC 0.5 mg
M080-1 Integrin alpha5 chain (CD49e) Tap.A12 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests
M110-0 CD9 Nyn.H3 Rat IgG2a purif. IP, IHC, WB 0.5 mg
M110-1 CD9 Nyn.H3 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests
M120-0 CD31 (PECAM-1) Pec.H3 Rat IgG1 purif. IP, IHC, WB 0.5 mg
M130-0 CD62P (P-selectin) Wug.E9 Rat IgG1 purif. IP, IHC 0.5 mg
M130-1 CD62P (P-selectin) Wug.E9 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests
M130-2 CD62P (P-selectin) Wug.E9 Rat IgG1 PE FC, IHC 1.5 ml; 300 tests
P140-1 fibrinogen polyclonal Rabbit IgG FITC FC, IHC 1.5 ml; 300 tests
P150-1 von Willebrand Factor (vWF) polyclonal Rabbit IgG FITC FC, IHC 1.5 ml; 300 tests
P180-1 negative control polyclonal Rabbit IgG FITC FC, IHC 1.0 ml; 200 tests
P190-1 negative control polyclonal Rat IgG FITC FC, IHC 1.0 ml; 200 tests
P190-2 negative control polyclonal Rat IgG PE FC, IHC 1.0 ml; 200 tests
P190-3 negative control polyclonal Rat IgG DyLight 649 FC, IHC 1.0 ml; 200 tests
R300 Antibodies for Mouse Plaet Depletion polyclonal anti-GPIb alpha Rat IgG purif. plaet depletion 0.5 mg
X488 Antibodies for In Vivo Mouse Plaet Labeling anti – GPIbbeta derivative Rat IgG DyLight 488 in vivo 100 µg
X649 Antibodies for In Vivo Mouse Plaet Labeling anti – GPIbbeta derivative Rat IgG DyLight 649 in vivo 100 µg
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