cytoskeleton Biochem试剂盒说明书

 

Cytoskeleton,Inc。很高兴为药物筛选,信号转导和细胞骨架研究提供广泛的试剂盒和产品。我们专注于生产纯化蛋白质和易于使用的试剂盒,以研究生物化学和细胞过程。我们的试剂盒可用于少量样品,用于基础研究或小屏幕(Biochem Kits™),也可用于大屏幕的高通量样品(CytoDYNAMIX Screens™)。

除了我们现有的产品,我们还在产品线中为微管,微管蛋白,运动蛋白,小G蛋白效应物,GAP,GEF和其他几种蛋白提供药物筛选服务。

 

Cytoskeleton的Biochem Kits™是用于分析细胞骨架生物化学和信号转导的不同方面的综合试剂盒。这些工具包通常构成出版物中图形的基础,请查看引文标签中的示例。该试剂盒附带了分析所需的所有试剂以及如何使用它们的详细说明,因此您可以在准备好试剂盒后立即进行分析!

 

· 测量蛋白质和药物对肌动蛋白聚合/解聚的影响。

· 发现和表征肌动蛋白结合蛋白(ABPs)。

· 在细胞或组织裂解物中定量G-肌动蛋白(单体)对F-肌动蛋白(聚合物)。

· 将设置时间从几个月缩短到几天。

许多出版物引用了在论文的材料和方法部分中使用Cytoskeleton的试剂盒。通常,引文与图形或图像形式的特定结果相关联,这有助于您,作者,您的发现。这表明套件可在短时间内生成出版物质量数据,从而有助于提高工作效率。肌动蛋白试剂盒的引用示例如下所示。个别产品页面上提供了更多引文。

 

肌动蛋白结合蛋白旋转分析Biochem试剂盒:兔骨骼肌肌动蛋白(Cat。#BK001)

Takeshita,N.,Ohta,A。和Horiuchi,H。(2005)。CsmA是一种具有肌球蛋白运动样结构域的V类几丁质合酶,通过与构巢曲霉中肌动蛋白细胞骨架的直接相互作用进行定位。摩尔。生物学。细胞16,1961-1970。

Banerjee,J。和Wedegaertner,PB(2004)。鉴定PDZ-RhoGEF中的新序列,其介导与肌动蛋白细胞骨架的相互作用。摩尔。生物学。Cell 15,1760-1775。

Kumar,N.,Zhao,P.,Tomar,A.,Galea,CA和Khurana,S。(2004)。绒毛蛋白与磷脂酰肌醇4,5-二磷酸的结合调节肌动蛋白细胞骨架。J. Biol。化学。279,3096-3110。

Torgler,CN,Narasimha,M.,Knox,AL,Zervas,CG,Vernon,MC和Brown,NH(2004)。Tensin稳定果蝇中的整合素粘合剂接触。开发。Cell 6,357-369。

Oliver,CJ,Terry-Lorenzo,RT,Elliott,E.,Bloomer,W​​A,Li,S.,Brautigan,DL,Colbran,RJ和Shenolikar,S。(2002)。将蛋白磷酸酶1(PP1)靶向肌动蛋白细胞骨架:neurabin I / PP1复合物调节细胞形态。摩尔。细胞。生物学。22,4690-4701。

Hildebrand,JD和Soriano,P。(1999)。Shroom是一种含有PDZ结构域的肌动蛋白结合蛋白,是小鼠神经管形态发生所必需的。细胞99,485-497。

肌动蛋白聚合生物化学试剂盒(荧光形式):兔骨骼肌肌动蛋白(Cat。#BK003)

Takamiya,R.,Takahashi,M.,Park,YS,Tawara,Y.,Fujiwara,N.,Miyamoto,Y.,Gu,J.,Suzuki,K。和Taniguchi,N。(2005)。神经母细胞瘤细胞中突变的Cu,Zn-SOD的过度表达导致细胞骨架改变。上午。J. Physiol。288,C253-259。

Kumar,N.,Tomar,A.,Parrill,AL和Khurana,S。(2004)。功能性解剖和分子表征的钙敏感肌动蛋白封闭和肌动蛋白解聚位点在绒毛蛋白。J. Biol。化学。279,45036-45046。

Fontao,L.,Geerts,D.,Kuikman,I.,Koster,J.,Kramer,D。和Sonnenberg,A。(2001)。plectin与肌动蛋白的相互作用:通过肌动蛋白结合结构域的二聚化肌动蛋白丝交联的证据。J. Cell Sci。114,2065-2076。

Zhai,L.,Zhao,P.,Panebra,A.,Guerrerio,AL和Khurana,S。(2001)。绒毛蛋白的酪氨酸磷酸化调节肌动蛋白细胞骨架的组织。J. Biol。化学。276,36163-36167。

Blader,IJ,Cope,MJ,Jackson,TR,Profit,AA,Greenwood,AF,Drubin,DG,Prestwich,GD和Theibert,AB(1999)。GCS1是酿酒酵母中的Arf鸟苷三磷酸酶活化蛋白,是体内正常肌动蛋白细胞骨架组织所必需的,并且在体外刺激肌动蛋白聚合。摩尔。生物学。Cell 10,581-596。

肌动蛋白结合蛋白旋转分析Biochem试剂盒:人血小板肌动蛋白(Cat。#BK013)

Gohla,A.,Birkenfeld,J。和Bokoch,GM(2005)。Chronophin是一种新型HAD型丝氨酸蛋白磷酸酶,可调节cofilin依赖性肌动蛋白动力学。纳特。细胞生物学。7,21-29。

Falcon-Perez,JM,Starcevic,M.,Gautam,R。和Dell'Angelica,EC(2002)。BLOC-1,一种新的复合物,含有苍白素和弱蛋白,参与黑素体和血小板致密颗粒的生物发生。J. Biol。化学。277,28191-28199。

Zhai,L.,Zhao,P.,Panebra,A.,Guerrerio,AL和Khurana,S。(2001)。绒毛蛋白的酪氨酸磷酸化调节肌动蛋白细胞骨架的组织。J. Biol。化学。276,36163-36167。

Leung,CL,Sun,D.,Zheng,M.,Knowles,DR and Liem,RK(1999)。微管肌动蛋白交联因子(MACF):dystonin和肌营​​养不良蛋白的杂合体,可与肌动蛋白和微管细胞骨架相互作用。J. Cell Biol。147,1275-1286。

G-Actin / F-actin体内检测Biochem试剂盒(目录号BK037)

Rapier等,2010。癌细胞Int。10,24

Rapier等。(2010年)。细胞外基质微观形成驱动结肠癌细胞中细胞运动性和Rho A活性的关键变化。癌细胞10,24。

Meeks,MK,Ripley,ML,Jin,Z。和Rembold,CM(2005)。热休克蛋白20介导的力抑制毛喉素放松猪颈动脉。上午。J. Physiol。288,C633-639。

Zhang,W.,Wu,Y.,Du,L.,Tang,DD and Gunst,SJ(2005)。通过N-WASp激活Arp2 / 3复合物是肌动蛋白聚合和平滑肌收缩所必需的。上午。J. Physiol。288,C1145-1160。

Chen,G.,Raman,P.,Bhonagiri,P.,Strawbridge,AB,Pattar,GR和Elmendorf,JS(2004)。磷脂酰肌醇4,5-二磷酸对3T3-L1脂肪细胞持续暴露于胰岛素诱导的皮质丝状肌动蛋白丢失和胰岛素抵抗的保护作用。J. Biol。化学。279,39705-39709。

Tang,DD和Gunst,SJ(2004)。小GTP酶Cdc42在平滑肌的收缩刺激期间调节肌动蛋白聚合和张力发展。J. Biol。化学。279,51722-51728。

Searles CD,Ide L,Davis ME,Cai H,Weber M(2004)。肌动蛋白细胞骨架组织和细胞生长过程中内皮细胞一氧化氮合酶的转录后调节。Circulation Research 95,488-495。

Tu,Y.,Wu,S.,Shi,X.,Chen,K。和Wu,C。(2003)。Migfilin和Mig-2将细丝蛋白和肌动蛋白细胞骨架的细胞粘连与细胞形状调节功能联系起来。细胞113,37-47。

问题1:哪种试剂盒是检测肌动蛋白结合蛋白的*选择? 

答案1:  Cytoskeleton有两个肌动蛋白结合蛋白旋转分析试剂盒,是识别和表征肌动蛋白结合蛋白的理想选择。一个试剂盒使用肌肉肌动蛋白(Cat。#BK001),另一个试剂盒使用非肌肉肌动蛋白(Cat。#BK013)。两种测定均利用共沉淀来鉴定蛋白质是否与单体或丝状肌动蛋白结合以及蛋白质是否具有F-肌动蛋白切断或成束特性。为了检查与肌动蛋白单体结合的化合物或蛋白质,含有链霉抗生物素蛋白包被的孔的96孔板可以与生物素化肌动蛋白复合(Cat。#AB07)产生肌动蛋白单体捕获蛋白。然后可以将化合物,蛋白质或提取物与捕获蛋白一起温育,并且可以通过HRP检测系统使用针对目标化合物或蛋白质的抗体(或标记可以与测试蛋白缀合)来显现结合。请记住,缓冲液组成也很重要,因为它需要低盐(约50 mM)并含有CaCl 2(或其他二价阳离子)和ATP以维持肌动蛋白单体的稳定性。有关兼容缓冲液的示例,请参阅我们的一般肌动蛋白缓冲液配方(Cat。#BSA01-001)。或者,可以用GST标记感兴趣的蛋白质(避免还原剂)并与板缀合,然后与生物素化的肌动蛋白一起温育,并用HRP缀合的链霉抗生物素蛋白显现。

 

问题2:当我对感兴趣的肌动蛋白没有可用的芘结合物(例如心脏,平滑肌或细胞质肌动蛋白)时,如何用芘测定法测量肌动蛋白聚合? 

答案2:  为了检查未标记肌动蛋白的聚合,请点击此处查看使用过量未标记肌动蛋白(心肌,平滑肌或细胞质)加10%芘标记肌肉肌动蛋白的聚合方案。芘肌肉肌动蛋白不会以该测定中使用的浓度自身聚合,因此反应依赖于未标记的非肌肉肌动蛋白形成F-肌动蛋白。以这种方式,芘标记的肌肉肌动蛋白被吸收并聚合以充当用于聚合未标记的肌动蛋白的报道分子,其以更高的浓度存在。

 

问题3:如何从细胞或组织提取物中测量G-和F-肌动蛋白? 

答案3:  确定细胞或组织样本中游离球状肌动蛋白(G-肌动蛋白)含量与丝状肌动蛋白(F-肌动蛋白)含量的可重复和准确的方法是使用F-肌动蛋白和G-肌动蛋白部分的Western印迹定量。 。一般方法是使F-肌动蛋白稳定缓冲液中的细胞或组织均质化,然后高速离心以将F-肌动蛋白与G-肌动蛋白池分离。然后通过SDS-PAGE分离级分,并通过Western印迹定量肌动蛋白。终结果给出了准确的方法来确定掺入细胞骨架的F-肌动蛋白与细胞质中发现的G-肌动蛋白的比例。细胞骨架提供了一种简单易用的选择,可用G-肌动蛋白/ F-肌动蛋白体内检测试剂盒(Cat。#BK037)定量G-和F-肌动蛋白。该试剂盒提供了在不同处理条件下定量细胞或组织样品中单体和聚合肌动蛋白水平的所有必需试剂。将样品离心,然后将沉淀(F-肌动蛋白)和上清液(G-肌动蛋白)溶解于SDS上样缓冲液中并通过Western印迹处理。 

 

肌动蛋白结合蛋白旋转分析Biochem试剂盒:人血小板肌动蛋白

BK013

 

肌动蛋白结合蛋白旋转分析Biochem试剂盒:兔骨骼肌肌动蛋白

BK001

 

肌动蛋白聚合生物化学试剂盒(荧光形式):兔骨骼肌肌动蛋白

BK003

 

F-actin Visualization Biochem Kit(荧光格式)

BK005

 

G-Actin / F-actin In Vivo Assay Biochem Kit

BK037

 

 

 

肌动蛋白结合蛋白旋转分析Biochem试剂盒:人血小板肌动蛋白

 

 

窗体顶端

30-100次分析(Cat。#BK013)

窗体底端

产品用途包括

· 确定蛋白质是否与肌动蛋白的细丝或单体结合。

· 确定蛋白质是否捆绑F-肌动蛋白

· 测试与肌动蛋白结合的各种条件(例如适pH)或要求(例如二价阳离子要求)

简介
肌动蛋白结合蛋白降速测定试剂盒提供G-或F-肌动蛋白加阳性(α – 肌动蛋白)和阴性(牛血清白蛋白,BSA)结合对照蛋白。肌动蛋白结合发生在对肌动蛋白的任何部位具有亲和力时。可以通过使用降速法测量F-肌动蛋白结合。以这种方式,离心用于通过差异沉降将F-肌动蛋白与G-肌动蛋白分离。F-肌动蛋白结合蛋白将与肌动蛋白丝共沉淀并在离心管底部形成沉淀。可以检测具有F-肌动蛋白成束活性的蛋白质,因为它们将导致F-肌动蛋白以比正常更低的离心力沉降(14,000xg对150,000xg)。F-肌动蛋白切断蛋白,G-肌动蛋白结合蛋白或非肌动蛋白结合蛋白将留在上清液中。如果上清液中残留的G-肌动蛋白比阴性对照样品中的G-肌动蛋白更多,则可以预期切断蛋白质,并且应该通过在添加测试蛋白质之前和之后测量F-肌动蛋白长度分布来进一步测试该活性。G-肌动蛋白结合蛋白可以通过将测试蛋白添加到G-肌动蛋白并诱导聚合来测量,如果测试蛋白螯合G-肌动蛋白,那么在旋转测定期间,与对照相比,肌动蛋白将留在上清液中。

肌动蛋白可以以两种形式存在:球状亚单位(G-肌动蛋白)和丝状聚合物(F-肌动蛋白)(有关更多信息,请参见关于肌动蛋白页面)。两种形式的肌动蛋白都与细胞中过多的蛋白质相互作用。到目前为止,有超过50种不同类别的肌动蛋白结合蛋白(ABP),而且库存仍远未完成。肌动蛋白结合蛋白允许肌动蛋白细胞骨架快速响应细胞和细胞外信号,并且在许多细胞过程中对细胞骨架参与是*的。这些包括细胞形状和运动,肌肉收缩,细胞内运输,细胞发病机理和信号转导。

该试剂盒含有非肌肉肌动蛋白(Cat。#APHL99)。同样的试剂盒也可用于骨骼肌肌动蛋白(Cat。#AKL99),参见Cat。#BK001。骨骼肌肌动蛋白旋转试剂盒可能更适合用于研究来自肌肉组织的肌动蛋白结合蛋白。

试剂盒内容
该试剂盒包含足够的材料,可根据检测体积进行30-100次检测。包括以下试剂:

00001. 8 x250μg非肌肉肌动蛋白(Cat。#APHL99)。

00002. α- Actinin,阳性对照(Cat。#AT01)。

00003. BSA,阴性对照

00004. 一般肌动蛋白缓冲液(Cat。#BSA01)。

00005. 肌动蛋白聚合缓冲液(Cat。#BSA02

00006. F-肌动蛋白缓冲液。

00007. ATP,100 mM(Cat。#BSA04

00008. EGTA,0.5米

00009. 的MgCl 2,100毫

00010. Tris-HCl pH 6.5,1.0M

00011. Tris-HCl pH 7.5,100mM

00012. 手册详细的协议和广泛的故障排除指南

需要的设备

00001. 离心设置能够在4°C和24°C下容量为150,000 xg,体积容量为50-200μl。

00002. SDS-PAGE系统。

00003. 感兴趣的蛋白质的检测系统(考马斯有利于纯化的蛋白质,蛋白质印迹或银染色用于较低纯度或低丰度的测试蛋白质)。

00004. 用于光密度测定的凝胶扫描仪。

问题1:当使用非肌肉肌动蛋白结合蛋白生物化学试剂盒(Cat。#BK013或肌动蛋白结合蛋白生物化学试剂盒Cat。#BK001)时,制备肌动蛋白结合试验蛋白的*条件是什么?

答案1:  在肌动蛋白相容缓冲液(如HEPES或磷酸盐缓冲液)中使测试蛋白质达到尽可能高的浓度。高浓度(优选>20μM)是优化检测肌动蛋白和测试蛋白之间相互作用的机会所必需的。在某些情况下,K d肌动蛋白结合蛋白/肌动蛋白相互作用的(解离常数)可以低到不能通过颗粒的蛋白质测定检测到; 然而,对于肌动蛋白在反应混合物中的影响可能足以通过沉淀和上清液中的肌动蛋白量与阴性对照的差异来检测。我们还建议将测试蛋白质在150,000 xg下在4°C下离心1小时,以去除变性蛋白质和细胞碎片。如果已经采取其他步骤(例如亲和柱纯化)来纯化测试蛋白,则这不是必需的。高速离心后,取出上清液置于冰上。这是测试蛋白质原料。

 

总离子强度应低于50 mM,这是由于肌动蛋白结合的特性,因此它主要是离子的,因此会受到中盐和高盐缓冲液的影响。一个好的起始缓冲液是20 mM Hepes pH 7.4加20 mM KCl,加上蛋白酶抑制剂,磷酸酶抑制剂和任何必要的辅助因子。可以用20mM MgCl 2和10mM ATP 制备低盐肌动蛋白聚合10x缓冲液,从而使盐在终反应中保持低水平。 

 

需要考虑的其他问题是(i)测试蛋白辅助因子可能是必需的,(ii)测定中的因子可能是主动抑制相互作用,(iii)测试蛋白可能偏爱不同类型的肌动蛋白,或( iv)测试蛋白质在组织提取物中可以是低浓度的。

 

为了解决这些潜在问题中的一些,可以添加试剂以去除抑制剂,例如用于钙的EGTA(部件号BSEG-01)。在运动蛋白的情况下,ATP / Mg2 +组合将使运动与F-肌动蛋白解离,因此以1mM使用不可水解的类似物如AMPPNP。肌动蛋白亲和柱可用于浓缩和分离更多的测试蛋白。后,不同的pH条件可以改善肌动蛋白和肌动蛋白结合蛋白之间的结合(例如,α-辅肌动蛋白的适pH为7.0)。

 

问题2:非肌肉肌动蛋白结合蛋白生物化学试剂盒(Cat。#BK013)可用于测定非肌肉肌动蛋白和肌动蛋白结合蛋白之间的结合亲和力吗?

答案2:   结合亲和力(K d)可以通过旋转降低测定来估计,通过滴定测试蛋白质浓度在0.2和20μM之间,并找到原始蛋白质在颗粒中的一半的浓度。绘制百分比测试蛋白质结合对比反应中的原始浓度以获得以μM计的Kd。

 

问题3:细胞裂解液可以与非肌肉肌动蛋白结合蛋白生物化学试剂盒(Cat。#BK013)一起用作测试蛋白的来源吗?

答案3:是的,细胞裂解液可用作测试蛋白的来源。然而,细胞骨架不建议这样做,因为蛋白质的纯度和浓度通常太低而不能与肌动蛋白相互作用。此外,裂解物将含有额外的辅助蛋白和多种磷酸酶和蛋白酶,它们可以干扰或改变肌动蛋白和肌动蛋白结合蛋白之间的相互作用。如果要使用裂解物,我们建议如下:

 

尽管该试剂盒设计用于纯蛋白质或化合物,但一些研究人员已经添加了具有良好结果的提取物。通常研究人员使用过表达的蛋白质和类似过表达的野生型对照提取物。需要制备10mg / ml蛋白质提取物,然后将其1/3体积用于1mg / ml的2/3体积的肌动蛋白。以这种方式,存在足够高的蛋白质浓度以指示结合。提取缓冲液应为20mM Hepes缓冲液pH 7.4,20mM NaCl,加上蛋白质的任何辅因子,以及蛋白酶抑制剂混合物如Cat。#PIC02。也可加入磷酸酶抑制剂。用冰冷的缓冲液冲洗细胞,用注射器针头或其他装置弯曲25g裂解细胞。pH可以将肌动蛋白结合蛋白(ABP)的F-肌动蛋白结合特性改变0至100%。对于提取物制备,测试pH 6.5,7.5和8.5是个好主意。对照细胞系非常关键,因为潜在的ABP可能通过其他途径出现在颗粒中; 例如,变性或与其他细胞蛋白质聚集。总离子强度应保持在50 mM以下,以免使任何潜在的ABP脱盐。

 

抗体

· 概述

· 常见问题解答

Cytoskeleton Inc致力于为肌动蛋白和肌动蛋白结合蛋白提供高质量的抗体。我们在该系列产品中的所有抗体均经过QC Max™测试。已经测试QC Max TM抗体用于蛋白质印迹,原位和免疫沉淀。这样,您就可以准确了解每种抗体的用途,并确保购买适合您的抗体。如果您对我们的QC Max™抗体有关的详细信息,请点击下面列出的产品以获取更多详细信息。

问题1:在细胞和组织中染色F-肌动蛋白的*方法是什么?

答案2:   细胞骨架提供我们的Acti染色系列荧光标记的鬼笔环肽,可在固定细胞和组织样本中特异性染色F-肌动蛋白。Acti染色荧光鬼笔环肽探针非常明亮和稳定,为标记F-肌动蛋白提供了一种非常简单和经济的方法。鬼笔环肽用不同的绿色和红色荧光团(Cat。#PHDG1,PHDR1,PHDH1和PHDN1)标记。也可以使用肌动蛋白抗体,但通常那些也染色G-肌动蛋白,这产生高背景。

 

问题2:您对多克隆抗肌动蛋白抗体有何推荐稀释度?

答案2:   抗体的稀释度取决于所使用的细胞/组织类型和实验形式。对于蛋白质印迹,我们已经测试并推荐使用浓度为500ng / ml(1:1000稀释)的抗体。在该稀释度下,我们在非洲爪蟾 A6细胞,小鼠Swiss 3T3细胞,大鼠NRK细胞,人HeLa细胞和血小板细胞的细胞提取物中检测到肌动蛋白。对于免疫荧光,我们使用抗肌动蛋白抗体检测小鼠Swiss 3T3细胞中的肌动蛋白。抗体以2μg/ ml(1:500稀释)的浓度使用,然后与1:500稀释的抗兔罗丹明缀合的第二抗体一起温育。这些信息通常在图例中或我们的抗体数据表的“产品用途”部分中找到。

 

 

 

货号

品名

规格

品牌

BK165-S

Signal-Seeker™ SUMOylation 1 Detection Kit

(10 assay)

cytoskeleton

BK165

Signal-Seeker™ SUMOylation 1 Detection Kit

(30 assay)

cytoskeleton

BK163-S

Signal-Seeker™ Acetyl-Lysine Detection Kit

(10 assay)

cytoskeleton

BK163

Signal-Seeker™ Acetyl-Lysine Detection Kit

(30 assay)

cytoskeleton

BK160-S

Signal-Seeker™ Phosphotyrosine Detection Kit

(10 assay)

cytoskeleton

BK160

Signal-Seeker™ Phosphotyrosine Detection Kit

(30 assay)

cytoskeleton

BK162-S

Signal-Seeker™ SUMOylation 2/3 Detection Kit

(10 assay)

cytoskeleton

BK162

Signal-Seeker™ SUMOylation 2/3 Detection Kit

(30 assay)

cytoskeleton

BK161-S

Signal-Seeker™ Ubiquitination Detection Kit

(10 assay)

cytoskeleton

BK161

Signal-Seeker™ Ubiquitination Detection Kit

(30 assay)

cytoskeleton

AAC04-Beads

Acetyl-Lysine Affinity Beads

ea

cytoskeleton

APY03-Beads

Anti-Phosphotyrosine Affinity Beads

ea

cytoskeleton

ASM11-Beads

SUMOylation 1 Affinity Beads

ea

cytoskeleton

ASM24-Beads

SUMOylation 2/3 Affinity Beads

ea

cytoskeleton

UBA01-Beads

Ubiquitination Affinity Beads

ea

cytoskeleton

CIG02-Beads

Acetyl-Lysine Control Beads

ea

cytoskeleton

CIG03-Beads

SUMO1 IP Control Beads

ea

cytoskeleton

CIG01-Beads

IgG Control Beads

ea

cytoskeleton

CUB02-Beads

Ubiquitination Control Beads

ea

cytoskeleton

BLR01

BlastR™ Rapid Lysate Prep Kit

ea

cytoskeleton

BLR02

BlastR™ Rapid Filtration Kit

ea

cytoskeleton

AAC02

Acetyl Lysine Antibody Mouse Monoclonal (7B1)

ea

cytoskeleton

AAC03

Acetyl Lysine Antibody Mouse Monoclonal (19C4B2.1)

ea

cytoskeleton

AAC03-HRP

Acetyl Lysine-HRP Antibody Mouse Monoclonal (19C4B2.1)

ea

cytoskeleton

AAC01

Acetyl Lysine Antibody Mouse Monoclonal (3C6.08.20)

ea

cytoskeleton

APY03

Phosphotyrosine Antibody Mouse Monoclonal (27B10)

ea

cytoskeleton

APY03-HRP

Phosphotyrosine-HRP Antibody Mouse Monoclonal (27B10)

ea

cytoskeleton

ASM01

SUMO1 Antibody Mouse Monoclonal (Clone 5D8B16)

ea

cytoskeleton

ASM24

SUMO-2/3 Antibody Mouse Monoclonal (Clone 11G2)

ea

cytoskeleton

ASM23

SUMO-2/3 Antibody Mouse Monoclonal (Clone 12F3)

ea

cytoskeleton

ASM23-HRP

SUMO-2/3-HRP Antibody Mouse Monoclonal (Clone 12F3)

ea

cytoskeleton

AUB01

Ubiquitin Antibody Mouse Monoclonal

ea

cytoskeleton

AUB01-HRP

Ubiquitin-HRP Antibody Mouse Monoclonal

ea

cytoskeleton

PHDG1

Acti-stain 488 phalloidin

ea

cytoskeleton

PHDH1

Acti-stain 555 phalloidin

ea

cytoskeleton

PHDN1

Acti-stain 670 phalloidin

ea

cytoskeleton

BK001

Actin Binding Protein Spin-Down Assay Biochem Kit: rabbit skeletal muscle actin

ea

cytoskeleton

BK003

Actin Polymerization Biochem Kit (fluorescence format): rabbit skeletal muscle actin

ea

cytoskeleton

AKL95

Actin protein (>95% pure): rabbit skeletal muscle

ea

cytoskeleton

AD99

Actin protein (>99% pure): bovine cardiac muscle

ea

cytoskeleton

AS99

Actin protein (>99% pure): chicken gizzard muscle

ea

cytoskeleton

AKL99

Actin protein (>99% pure): rabbit skeletal muscle

ea

cytoskeleton

AKF99

Actin protein (pre-formed filaments): rabbit skeletal muscle

ea

cytoskeleton

AP05

Actin protein (pyrene labeled): rabbit skeletal muscle

ea

cytoskeleton

APHR

Actin protein (rhodamine): human platelet

ea

cytoskeleton

AR05

Actin protein (rhodamine): rabbit skeletal muscle

ea

cytoskeleton

AT01

Alpha-actinin protein: rabbit skeletal muscle

ea

cytoskeleton

RP01P

Arp2/3 protein complex: porcine brain

ea

cytoskeleton

CS-TFC01

Cardiac Thin Filament Complex (CTFC)

ea

cytoskeleton

CF01

Cofilin 1 protein: human recombinant

ea

cytoskeleton

HPG6

Gelsolin protein: Homo sapiens recombinant

ea

cytoskeleton

MH01

Heavy meromyosin protein

ea

cytoskeleton

CS-MH03

Heavy Meromyosin Protein (HMM Fragment)

ea

cytoskeleton

CS-MYS03

Myosin – cardiac S1 fragment

ea

cytoskeleton

CS-MYS04

Myosin – skeletal muscle S1 fragment

ea

cytoskeleton

CS-MYS05

Myosin – smooth muscle S1 fragment

ea

cytoskeleton

MY02

Myosin II protein: rabbit skeletal muscle

ea

cytoskeleton

MY03

Myosin protein: bovine cardiac muscle

ea

cytoskeleton

PR02

Profilin 1 protein: Untagged, human recombinant

ea

cytoskeleton

PHDR1

Rhodamine Phalloidin

ea

cytoskeleton

CY-SC001

SiR-Actin Kit

ea

cytoskeleton

CY-SC013

SiR700-Actin Kit

ea

cytoskeleton

VCG03

WASP protein VCA domain: GST tagged: human

ea

cytoskeleton

 

 

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

2:产品种类齐全,经营超过700多品牌,基本涵盖所有生物实验试剂耗材。

3:提供加急服务,货品一般1-2周到货。

4:富有竞争力的价格优势,绝大部分价格有优势。

5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等知药企。

6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批发,欢迎合作。

 

Cytoskeleton PHDG1-A说明书

 

Cytoskeleton,Inc。很高兴为药物筛选,信号转导和细胞骨架研究提供广泛的试剂盒和产品。我们专注于生产纯化蛋白质和易于使用的试剂盒,以研究生物化学和细胞过程。我们的试剂盒可用于少量样品,用于基础研究或小屏幕(Biochem Kits™),也可用于大屏幕的高通量样品CytoDYNAMIX Screens™)。

除了我们现有的产品,我们还在产品线中为微管,微管蛋白,运动蛋白,小G蛋白效应物,GAP,GEF和其他几种蛋白提供药物筛选服务。

Acti-stain 488 phalloidin  Acti-stain 488鬼笔环肽

 

(Cat. # PHDG1-A)   300 Slides

产品用途包括

· 固定组织切片和组织培养细胞制剂中肌动蛋白丝的荧光染色。  注意:与许多肌动蛋白抗体不同,Acti-stain™488鬼笔环肽仅与F-肌动蛋白结合,导致低背景荧光。此外,通过Acti染色结合F-肌动蛋白在物种之间没有明显差异。

· 体外稳定荧光肌动蛋白丝的制备。

 

肌动蛋白染色在确定活细胞和固定细胞中细胞骨架的结构和功能方面非常有用。肌动蛋白细胞骨架在活细胞中是非常动态和不稳定的结构,但是在探测或染色肌动蛋白结构之前可以用多聚甲醛固定。 

 

材料

鬼笔环肽是来自蘑菇伞形毒蕈的七氨基酸肽毒素,其特异性地并且以高亲和力(Kd 20nM)结合至肌动蛋白的聚合形式(F-肌动蛋白)。鬼笔环肽将肌动蛋白聚合的临界浓度降低至小于1μg/ ml,从而充当聚合增强剂。鬼笔环肽已经用专有的绿色荧光染料标记,使其可用于染色组织培养细胞和组织切片中的肌动蛋白丝(1,参见图1)和无细胞制剂。Acti-stain™488鬼笔环肽标记的肌动蛋白丝保留了未标记肌动蛋白的许多功能特征,包括它们与肌球蛋白相互作用的能力。Actin-stain TM 488鬼笔环肽以橙色固体形式提供。 

注意:鬼笔环肽是有毒的,必须小心处理(LD50人= 2mg / Kg)。

 

 

示例结果和规格

 

 

图1.在瑞士3T3细胞中用Acti-stain TM 488染色的肌动蛋白应力纤维。

 

图2. Acti-stain™鬼笔环肽的发射和激发扫描

 

 

 

 

 

 

 

用抗 – 纽蛋白(红色),Dapi(蓝色细胞核)和F-肌动蛋白染色的Swiss 3T3细胞用Acti-stain TM 488(绿色F-肌动蛋白,Cat。#PHDG1)染色。

 

Acti-stain TM 488的吸光度和荧光扫描。将标记的鬼笔环肽稀释到甲醇中,并分别在350-750和500-750nm之间扫描其吸光度和激发光谱。吸光度峰值在500nm处,荧光在550nm处。

 

Acti-stain鬼笔环肽是可用的明显的鬼笔环肽。表1描述了它们对F-肌动蛋白的亮度,光稳定性,背景和亲和力常数。将这些性能特征与其他荧光鬼笔环肽进行比较,您将看到使用Acti-stain™来满足肌动蛋白染色要求的优势。

表1.荧光鬼笔环肽的生物化学特征

共轭

货号

波长(Ex / Em)

亮度(AFU *)

光漂白的稳定性(以秒为单位的半衰期**)

背景 (总AFU在100nM时的百分比**)

亲和力(Kd,纳摩尔)

荧光毒伞素

na

485/535 FITC滤波器组

432

6

22

72 +/- 12

Acti-stain™488

PHDG1

485/535 FITC滤波器组

832

57

55 +/- 8

Acti-stain™535

PHDR1

535/585 TRITC滤波器组

430

27

12

36 +/- 7

Acti-stain™555

PHDH1

535/585 TRITC滤波器组

551

46

16

63 +/- 8

Acti-stain™670

PHDN1

640/680 Cy5滤光片组

332

8

18

50 +/- 12

* = AFU通过定量细胞成像测量。** =在没有抗褪色的情况下在染色的Swiss 3T3细胞中测量。

 

引用:

oitbak等,2011。微小RNA在神经干细胞支持内皮形态发生中的作用。血管细胞v 3,p 25。

 

Yuan et al。,2012。使用同量异位标签对胺代谢物进行亚秒级绝dui定量,以发现哺乳动物细胞中的通路活化。肛门。化学第84页,第2892-2899页。

 

问题1:我可以使用荧光标记的鬼笔环肽染色活细胞中的F-肌动蛋白吗?

答案1:  不幸的是,不,鬼笔环肽不能用于在活细胞中染色F-肌动蛋白。鬼笔环肽用于在固定细胞中染色F-肌动蛋白。荧光鬼笔环肽仅与F-肌动蛋白的天然四级结构结合,提供低背景。鬼笔环肽结合的正确固定条件是在PBS中的3.7%(v / v)多聚甲醛10分钟,因为它保留了鬼笔环肽的高亲和力结合所必需的季铵蛋白结构。甲醇固定破坏天然构象,因此不适合用鬼笔环肽染色F-肌动蛋白。为了监测活细胞中的肌动蛋白动力学,建议微量注射罗丹明标记的肌动蛋白(Cat。#APHR或AR05)。有关更多信息,请参阅这些数据表。

 

问题2:哪种荧光标记的鬼笔环肽稳定/亮?

答案2:  Acti-stains中亮和稳定的是Acti-stain 488(绿色荧光; Cat。#PHDG1)。有关我们所有Acti-stains的更多信息,请参阅下表。 

共轭

猫。#

波长(Ex / EM)

亮度(AFU)

光漂白的稳定性*(1/2秒寿命)

背景(总AFU在100 nM时的百分比)

Acti-stain™488

PHDG1

535分之485

832

57

Acti-stain™535

PHDR1

585分之535

430

27

12

Acti-stain™555

PHDH1

585分之535

551

46

16

Acti-stain™670

PHDN1

六百八十零分之六百四十

332

8

18

* =在没有抗褪色的情况下测量。

 

 

货号

品名

规格

品牌

BK165-S

Signal-Seeker™ SUMOylation 1 Detection Kit

(10 assay)

cytoskeleton

BK165

Signal-Seeker™ SUMOylation 1 Detection Kit

(30 assay)

cytoskeleton

BK163-S

Signal-Seeker™ Acetyl-Lysine Detection Kit

(10 assay)

cytoskeleton

BK163

Signal-Seeker™ Acetyl-Lysine Detection Kit

(30 assay)

cytoskeleton

BK160-S

Signal-Seeker™ Phosphotyrosine Detection Kit

(10 assay)

cytoskeleton

BK160

Signal-Seeker™ Phosphotyrosine Detection Kit

(30 assay)

cytoskeleton

BK162-S

Signal-Seeker™ SUMOylation 2/3 Detection Kit

(10 assay)

cytoskeleton

BK162

Signal-Seeker™ SUMOylation 2/3 Detection Kit

(30 assay)

cytoskeleton

BK161-S

Signal-Seeker™ Ubiquitination Detection Kit

(10 assay)

cytoskeleton

BK161

Signal-Seeker™ Ubiquitination Detection Kit

(30 assay)

cytoskeleton

AAC04-Beads

Acetyl-Lysine Affinity Beads

ea

cytoskeleton

APY03-Beads

Anti-Phosphotyrosine Affinity Beads

ea

cytoskeleton

ASM11-Beads

SUMOylation 1 Affinity Beads

ea

cytoskeleton

ASM24-Beads

SUMOylation 2/3 Affinity Beads

ea

cytoskeleton

UBA01-Beads

Ubiquitination Affinity Beads

ea

cytoskeleton

CIG02-Beads

Acetyl-Lysine Control Beads

ea

cytoskeleton

CIG03-Beads

SUMO1 IP Control Beads

ea

cytoskeleton

CIG01-Beads

IgG Control Beads

ea

cytoskeleton

CUB02-Beads

Ubiquitination Control Beads

ea

cytoskeleton

BLR01

BlastR™ Rapid Lysate Prep Kit

ea

cytoskeleton

BLR02

BlastR™ Rapid Filtration Kit

ea

cytoskeleton

AAC02

Acetyl Lysine Antibody Mouse Monoclonal (7B1)

ea

cytoskeleton

AAC03

Acetyl Lysine Antibody Mouse Monoclonal (19C4B2.1)

ea

cytoskeleton

AAC03-HRP

Acetyl Lysine-HRP Antibody Mouse Monoclonal (19C4B2.1)

ea

cytoskeleton

AAC01

Acetyl Lysine Antibody Mouse Monoclonal (3C6.08.20)

ea

cytoskeleton

APY03

Phosphotyrosine Antibody Mouse Monoclonal (27B10)

ea

cytoskeleton

APY03-HRP

Phosphotyrosine-HRP Antibody Mouse Monoclonal (27B10)

ea

cytoskeleton

ASM01

SUMO1 Antibody Mouse Monoclonal (Clone 5D8B16)

ea

cytoskeleton

ASM24

SUMO-2/3 Antibody Mouse Monoclonal (Clone 11G2)

ea

cytoskeleton

ASM23

SUMO-2/3 Antibody Mouse Monoclonal (Clone 12F3)

ea

cytoskeleton

ASM23-HRP

SUMO-2/3-HRP Antibody Mouse Monoclonal (Clone 12F3)

ea

cytoskeleton

AUB01

Ubiquitin Antibody Mouse Monoclonal

ea

cytoskeleton

AUB01-HRP

Ubiquitin-HRP Antibody Mouse Monoclonal

ea

cytoskeleton

PHDG1

Acti-stain 488 phalloidin

ea

cytoskeleton

PHDH1

Acti-stain 555 phalloidin

ea

cytoskeleton

PHDN1

Acti-stain 670 phalloidin

ea

cytoskeleton

BK001

Actin Binding Protein Spin-Down Assay Biochem Kit: rabbit skeletal muscle actin

ea

cytoskeleton

BK003

Actin Polymerization Biochem Kit (fluorescence format): rabbit skeletal muscle actin

ea

cytoskeleton

AKL95

Actin protein (>95% pure): rabbit skeletal muscle

ea

cytoskeleton

AD99

Actin protein (>99% pure): bovine cardiac muscle

ea

cytoskeleton

AS99

Actin protein (>99% pure): chicken gizzard muscle

ea

cytoskeleton

AKL99

Actin protein (>99% pure): rabbit skeletal muscle

ea

cytoskeleton

AKF99

Actin protein (pre-formed filaments): rabbit skeletal muscle

ea

cytoskeleton

AP05

Actin protein (pyrene labeled): rabbit skeletal muscle

ea

cytoskeleton

APHR

Actin protein (rhodamine): human platelet

ea

cytoskeleton

AR05

Actin protein (rhodamine): rabbit skeletal muscle

ea

cytoskeleton

AT01

Alpha-actinin protein: rabbit skeletal muscle

ea

cytoskeleton

RP01P

Arp2/3 protein complex: porcine brain

ea

cytoskeleton

CS-TFC01

Cardiac Thin Filament Complex (CTFC)

ea

cytoskeleton

CF01

Cofilin 1 protein: human recombinant

ea

cytoskeleton

HPG6

Gelsolin protein: Homo sapiens recombinant

ea

cytoskeleton

MH01

Heavy meromyosin protein

ea

cytoskeleton

CS-MH03

Heavy Meromyosin Protein (HMM Fragment)

ea

cytoskeleton

CS-MYS03

Myosin – cardiac S1 fragment

ea

cytoskeleton

CS-MYS04

Myosin – skeletal muscle S1 fragment

ea

cytoskeleton

CS-MYS05

Myosin – smooth muscle S1 fragment

ea

cytoskeleton

MY02

Myosin II protein: rabbit skeletal muscle

ea

cytoskeleton

MY03

Myosin protein: bovine cardiac muscle

ea

cytoskeleton

PR02

Profilin 1 protein: Untagged, human recombinant

ea

cytoskeleton

PHDR1

Rhodamine Phalloidin

ea

cytoskeleton

CY-SC001

SiR-Actin Kit

ea

cytoskeleton

CY-SC013

SiR700-Actin Kit

ea

cytoskeleton

VCG03

WASP protein VCA domain: GST tagged: human

ea

cytoskeleton

 

 

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

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cytoskeleton代理


cytoskeleton代理

简要描述:cytoskeleton是一家专业从事生产在细胞骨架研究中有用的蛋白,试剂和试剂盒的公司。公司从事细胞骨架蛋白工作有多年的工作经验,保证提供高质量的产品

详细介绍

产品咨询

 美国Cytoskeleton公司自1993年成立以来,不断扩大产品线,为药物开发、信号转导和细胞骨架方面的研究提供高质量的产品。该公司专著于生产纯化蛋白和使用方便的试剂盒,用于研究生化和细胞信号过程。G-LISATM系列试剂盒用ELISA为基础的方法在3小时之内快捷准确地测定小G蛋白的活性。

   

货号 品名 规格 目录价(¥)
AAN01-A Anti-actin antibody (rabbit polyAb) + control 1 x 100 µg ¥2,786.00
AAN01-B Anti-actin antibody (rabbit polyAb) + control 3 x 100 µg ¥7,210.00
AB07-A Actin protein (biotin, rabbit skeletal muscle) 5 x 20 µg ¥3,150.00
AB07-C Actin protein (biotin, rabbit skeletal muscle) 20 x 20 µg ¥8,890.00
AB07-D Actin protein (biotin, rabbit skeletal muscle) Large quantities 请询价
ACD03-A Anti-Cdc42 Mouse Monoclonal Antibody  1 x 100 µg ¥3,430.00
ACD03-B Anti-Cdc42 Mouse Monoclonal Antibody  3 x 100 µg ¥8,330.00
ACFL02-A Cofilin antibody (host rabbit) + control 1 x 50 µg ¥3,430.00
ACFL02-B Cofilin antibody (host rabbit) + control 3 x 50 µg ¥8,330.00
AD99-A Actin protein (bovine cardiac muscle, >99% pure) 1 x 1 mg ¥1,190.00
AD99-B Actin protein (bovine cardiac muscle, >99% pure) 5 x 1 mg ¥5,250.00
AD99-C Actin protein (bovine cardiac muscle, >99% pure) 20 x 1 mg ¥19,530.00
ADV01-A Advanced Protein Assay (5X conc) 1 x 500 ml ¥2,310.00
ADV01-B Advanced Protein Assay (5X conc) 3 x 500 ml ¥6,300.00
ADV02-A Precision Red Advanced Protein Assay (1X conc) 1 x 500 ml ¥1,386.00
ADV02-B Precision Red Advanced Protein Assay (1X conc) 3 x 500 ml ¥4,130.00
AFB01-A Fibrillarin monoclonal antibody 1 x 100 µg ¥3,990.00
AFB01-B Fibrillarin monoclonal antibody 3 x 100 µg ¥8,750.00
AKF99-A Pre-formed actin filaments (rabbit skeletal muscle) 1 x 1 mg ¥2,100.00
AKF99-B Pre-formed actin filaments (rabbit skeletal muscle) 5 x 1 mg ¥8,330.00
AKF99-C Pre-formed actin filaments (rabbit skeletal muscle) 20 x 1 mg ¥21,700.00
AKIN01-A Kinesin antibody pan-specific (rabbit polyAb) + control 1 x 50 µg ¥3,990.00
AKIN01-B Kinesin antibody pan-specific (rabbit polyAb) + control 3 x 50 µg ¥8,750.00
AKIN04-A CenPE motor antibody (host rabbit) + control 1 x 50 µg ¥3,990.00
AKIN04-B CenPE motor antibody (host rabbit) + control 3 x 50 µg ¥8,750.00
AKIN05-A MCAK motor antibody (host rabbit) + control 1 x 50 µg ¥3,990.00
AKIN05-B MCAK motor antibody (host rabbit) + control 3 x 50 µg ¥8,750.00
AKIN06-A MKLP1 motor antibody (host rabbit) + control 1 x 50 µg ¥3,990.00
AKIN06-B MKLP1 motor antibody (host rabbit) + control 3 x 50 µg ¥8,750.00
AKIN09-A KIF3C motor antibody (host rabbit) + control 1 x 50 µg ¥3,990.00
AKIN09-B KIF3C motor antibody (host rabbit) + control 3 x 50 µg ¥8,750.00
AKIN11-A KIF1C motor antibody (host rabbit) + control 1 x 50 µg ¥3,990.00
AKIN11-B KIF1C motor antibody (host rabbit) + control 3 x 50 µg ¥8,750.00
AKIN12-A Kid motor antibody (host rabbit) + control 1 x 50 µg ¥3,990.00
AKIN12-B Kid motor antibody (host rabbit) + control 3 x 50 µg ¥8,750.00
AKIN13-A Klp2 motor antibody (host rabbit) + control 1 x 50 µg ¥3,990.00
AKIN13-B Klp2 motor antibody (host rabbit) + control 3 x 50 µg ¥8,750.00
AKL95-B Actin protein (rabbit skeletal muscle, >95% pure) 1 x 1 mg ¥1,050.00
AKL95-C Actin protein (rabbit skeletal muscle, >95% pure) 5 x 1 mg ¥4,690.00
AKL95-D Actin protein (rabbit skeletal muscle, >95% pure) 10 x 1 mg ¥8,190.00
AKL95-E Actin protein (rabbit skeletal muscle, >95% pure) 20 x 1 mg ¥13,650.00
AKL95-XL Actin protein (rabbit skeletal muscle, >95% pure) Large quantities 请询价
AKL99-A Actin protein (rabbit skeletal muscle, >99% pure) 4 x 250 µg ¥2,450.00
AKL99-B Actin protein (rabbit skeletal muscle, >99% pure) 2 x 1 mg ¥2,786.00
AKL99-C Actin protein (rabbit skeletal muscle, >99% pure) 5 x 1 mg ¥5,530.00
AKL99-D Actin protein (rabbit skeletal muscle, >99% pure) 10 x 1 mg ¥9,730.00
AKL99-E Actin protein (rabbit skeletal muscle, >99% pure) 20 x 1 mg ¥17,500.00
AKL99-XL Actin protein (rabbit skeletal muscle, >99% pure) Large quantities 请询价
AP05-A Actin protein (pyrene, rabbit skeletal muscle) 1 x 1 mg ¥2,590.00
AP05-B Actin protein (pyrene, rabbit skeletal muscle) 5 x 1 mg ¥9,310.00
AP05-XL Actin protein (pyrene, rabbit skeletal muscle) Large quantities 请询价
APHL95-C Actin protein (human plaet non-muscle, >95% pure) 1 x 1 mg ¥1,386.00
APHL95-D Actin protein (human plaet non-muscle, >95% pure) 5 x 1 mg ¥6,440.00
APHL95-E Actin protein (human plaet non-muscle, >95% pure) 10 x 1 mg ¥11,970.00
APHL99-A Actin protein (human plaet non-muscle, >99% pure) 2 x 250 µg ¥2,310.00
APHL99-C Actin protein (human plaet non-muscle, >99% pure) 1 x 1 mg ¥3,290.00
APHL99-E Actin protein (human plaet non-muscle, >99% pure) 5 x 1 mg ¥11,550.00
APHL99-XL Actin protein (human plaet non-muscle, >99% pure) Large quantities 请询价
APHR-A Actin protein (rhodamine, human plaet non-muscle) 4 x 10 µg ¥3,290.00
APHR-C Actin protein (rhodamine, human plaet non-muscle) 20 x 10 µg ¥10,150.00
APHR-D Actin protein (rhodamine, human plaet non-muscle) Large quantities 请询价
APUF01-A Profilin polyclonal antibody (host rabbit) + control 1 x 50 µg ¥3,430.00
APUF01-B Profilin polyclonal antibody (host rabbit) + control 3 x 50 µg ¥8,330.00
AR05-B Actin protein (rhodamine, rabbit skeletal muscle) 10 x 20 µg ¥2,730.00
AR05-C Actin protein (rhodamine, rabbit skeletal muscle) 20 x 20 µg ¥5,530.00
AR05-D Actin protein (rhodamine, rabbit skeletal muscle) Large quantities 请询价
ARC03-A Anti-Rac1 specific mouse MAb 100 µg ¥3,990.00
ARC03-B Anti-Rac1 specific mouse MAb 300 µg ¥8,750.00
ARH03-A Anti-RhoA antibody (mouse MAb) + control. 100 µg ¥2,786.00
ARH03-B Anti-RhoA antibody (mouse MAb) + control. 300 µg ¥6,650.00
AS99-A Actin protein (chicken gizzard muscle, >99% pure) 1 x 1 mg ¥1,190.00
AS99-B Actin protein (chicken gizzard muscle, >99% pure) 5 x 1 mg ¥5,250.00
AS99-C Actin protein (chicken gizzard muscle, >99% pure) 20 x 1 mg ¥19,530.00
AT01-A Alpha-actinin (rabbit skeletal muscle) 2 x 50 µg ¥3,150.00
AT01-C Alpha-actinin (rabbit skeletal muscle) 10 x 50 µg ¥10,150.00
ATN02-A Anti-tubulin polyAb pan specific (sheep) + control 1 x 100 µg ¥1,330.00
ATN02-B Anti-tubulin polyAb pan specific (sheep) + control 3 x 100 µg ¥5,530.00
BK001 Actin Binding Protein Spin-Down Assay Kit KIT 30-100 assays ¥8,330.00
BK003 Actin Polymerization Biochem Kit  KIT 30-100 assays ¥9,590.00
BK004P / CDS01P Tubulin Polymerization Assay Kit (>97% pure porcine brain tubulin) KIT 24 assays ¥7,700.00
BK005 F-Actin Visualization Kit KIT 300 assays ¥3,990.00
BK006P / CDS03P Tubulin Polymerization Assay Kit (>99% pure porcine brain tubulin) KIT 24 assays ¥11,900.00
BK007R Fluorescent Microtubules Biochem Kit (Rhodamine) KIT 50-200 assays ¥6,790.00
BK008 Ras Activation Assay Biochem Kit KIT 50 assays ¥9,100.00
BK010S Plant Tubulin Polymerization Kit (soybean tubulin) KIT 30 assays ¥25,900.00
BK011P Tubulin Polymerization Assay (fluoresc-96assays porcine brain tubulin) KIT 96 assays ¥11,900.00
BK013 Actin Binding Protein Spin-Down Assay Kit (Non-muscle) KIT 30-100 assays ¥9,100.00
BK015 Microtubules / Tubulin Biochem Kit  KIT 8-200 assays ¥5,950.00
BK027 Kinesin/Dynein Motility Kit (Fluorescence) KIT 25 assays ¥11,130.00
BK029 Microtubule Binding Protein Spin Down Assay Kit KIT 30-100 assays ¥8,330.00
BK034 Cdc42 Activation Assay Kit KIT 50 assays ¥9,100.00
BK035 Rac Activation Assay Kit KIT 50 assays ¥9,100.00
BK036 Rho Activation Assay Kit KIT 80 assays ¥9,100.00
BK037 G-Actin : F-Actin In Vivo Assay Kit KIT 30-100 assays ¥9,100.00
BK038 Microtubule / Tubulin In Vivo Assay Kit KIT 30-100 assays ¥9,100.00
BK040 RalA Activation Assay Biochem Kit KIT 50 assays ¥9,100.00
BK050 PhosFree Phosphate Assay (>500ug/ml protein reactions) KIT 1000 assays ¥3,430.00
BK051 ATPase ELIPA (Enzyme Linked Inorganic Phosphate Assay TM) KIT 96 assays ¥3,430.00
BK052 GTPase ELIPA (Enzyme Linked Inorganic Phosphate Assay TM) KIT 96 assays ¥3,430.00
BK053 HTS Kinesin ATPase Endpoint Assay KIT 1000 assays ¥9,100.00
BK054 CytoPhos (TM) Phosphate Assay (1-500ug/ml protein reactions) KIT 1000 assays ¥3,430.00
BK060 Kinesin ELIPA (Enzyme Linked Inorganic Phosphate Assay TM) KIT 96 assays ¥9,100.00
BK100 GEF Exchange Assay KIT 60-300 assays ¥7,700.00
BK105 GAP Assay Kit KIT 80-160 assays ¥7,700.00
BK121 RhoA G-LISA Activation Assay (luminescence) KIT 96 assays ¥10,500.00
BK124 RhoA G-LISA Activation Assay (absorbance) KIT 96 assays ¥10,500.00
BK125 Rac1,2,3 G-LISA Activation Assay Kit KIT 96 assays ¥10,500.00
BK126 Rac1 G-LISA Activation Assay Kit KIT 96 assays ¥10,500.00
BK127 Cdc42 G-LISA Activation Assay Kit KIT 96 assays ¥10,500.00
BK128 Rac1 G-LISA Activation Assay Kit KIT 96 assays ¥10,500.00
BK129 RalA G-LISA Activation Assay Kit KIT 96 assays ¥10,500.00
BK131 Ras G-LISA Activation Assay Kit KIT 96 assays ¥10,500.00
BK150 RhoA ELISA Kit KIT 96 assays ¥5,250.00
BM01-A BimC Motor Domain (Asp.nid.) 2 x 25 µg ¥4,186.00
BM01-B BimC Motor Domain (Asp.nid.) 10 x 25 µg ¥13,930.00
BM01-XL BimC Motor Domain (Asp.nid.) Bulk discount avail. 1 x 1 mg ¥23,730.00
BS01 GTP gamma S 1 x 500 µg ¥910.00
BSA01-001 General Actin Buffer 1 x 10 ml ¥406.00
BSA01-010 General Actin Buffer 1 x 100 ml ¥1,386.00
BSA02-001 Actin Polymerization Buffer (10x stock) 1 x 2 ml ¥406.00
BSA04-001 ATP (100mM Stock Solution) 1 x 1 ml ¥406.00
BSM05-04 Perfusion Chambers (25 slides, + coverslip, acid washed) box of 25 ¥2,380.00
BST01-001 General Tubulin Buffer 1 x 10 ml ¥406.00
BST01-010 General Tubulin Buffer 1 x 100 ml ¥1,386.00
BST01-100 General Tubulin Buffer 1 x 1000 ml ¥4,130.00
BST05-001 Tubulin Glycerol Buffer 1 x 10 ml ¥406.00
BST06-001 GTP (100mM Stock for Motor Activity) 1 x 100 µl ¥406.00
BST06-010 GTP (100mM Stock for Motor Activity) 10 x 100 µl ¥1,960.00
C17G01-A Cdc42-GST protein: dominant negative 1 x 25 µg  ¥3,150.00
C17G01-C Cdc42-GST protein: dominant negative 4 x 25 µg ¥7,700.00
C6101-A Cdc42-His Protein: constituitively active 1 x 10 µg ¥3,150.00
C6101-C Cdc42-His Protein: constituitively active 4 x 10 µg ¥7,700.00
CD01-A Cdc42-His Protein: Wild type 1 x 100 µg ¥3,150.00
CD01-C Cdc42-His Protein: Wild type 3 x 100 µg ¥7,700.00
CD01-XL Cdc42-His Protein: Wild type 1 x 1 mg ¥13,300.00
CDG01-C Cdc42-GST Protein: Wild type 8 x 25 µg ¥5,530.00
CF01-A Cofilin (Human Recombinant) 1 x 100 µg ¥2,730.00
CF01-C Cofilin (Human Recombinant) 4 x 100 µg ¥8,890.00
CN01-A Rho Activator 5 x 10 units ¥2,590.00
CN01-B Rho Activator 20 x 10 units ¥8,750.00
CN02-A Rac and Cdc42 Activator 5 x 10 units ¥2,590.00
CN02-B Rac and Cdc42 Activator 20 x 10 units ¥8,750.00
CN03-A Rho activator II 3 x 20 µg ¥2,520.00
CN03-B Rho activator II 9 x 20 µg ¥6,720.00
CN04-A Rho/Rac/Cdc42 Activator I 3 x 20 µg ¥2,520.00
CN04-B Rho/Rac/Cdc42 Activator I 9 x 20 µg ¥6,720.00
CN06-A Rho Pathway Inhibitor I 5 x 10 units ¥924.00
CN06-B Rho Pathway Inhibitor I 20 x 10 units ¥2,800.00
CP01-A CENP-E Motor domain (Hm.sap.) 2 x 25 µg ¥4,186.00
CP01-B CENP-E Motor domain (Hm.sap.) 10 x 25 µg ¥13,930.00
CP01-XL CENP-E Motor domain (Hm.sap.) (Bulk discount avail.) 1 x 1 mg ¥23,730.00
CR01-A Chromokinesin Motor Domain (Hm.sap.) 2 x 25 µg ¥4,186.00
CR01-B Chromokinesin Motor Domain (Hm.sap.) 10 x 25 µg ¥13,930.00
CT03-A C3 transferase protein 1 x 25 µg ¥2,786.00
CT03-B C3 transferase protein 2 x 25 µg ¥5,530.00
CT03-C C3 transferase protein 4 x 25 µg ¥8,890.00
CT04-A Cell Permeable Rho Inhibitor (C3 Trans based)) 1 x 20 µg ¥2,590.00
CT04-B Cell Permeable Rho Inhibitor (C3 Trans based)) 5 x 20 µg ¥7,350.00
CT04-C Cell Permeable Rho Inhibitor (C3 Trans based)) 20 x 20 µg ¥20,300.00
EG01-A Eg5 Motor Domain (Hm.sap.) 2 x 25 µg ¥4,186.00
EG01-B Eg5 Motor Domain (Hm.sap.) 10 x 25 µg ¥13,930.00
EG01-XL Eg5 Motor Domain (Hm.sap.) (Bulk discount avail.) 1 x 1 mg ¥23,730.00
EG02-A Eg5 Homolog Motor Domain (Asp.fum.) 2 x 15 µg ¥4,186.00
EG02-B Eg5 Homolog Motor Domain (Asp.fum.) 10 x 15 µg ¥13,930.00
EG02-XL Eg5 Homolog Motor Domain (Asp.fum.) 1 x 1 mg ¥23,730.00
F001 Fungal Cell Tubulin (Agaricus bisporus) 1 x 250 µg ¥10,220.00
FNR01-A Fibronectin Rhodamine (bovine plasma) 5 x 20 µg ¥3,150.00
FNR01-B Fibronectin Rhodamine (bovine plasma) 20 x 20 µg ¥9,100.00
FNR02-A Fibronectin HiLyte488 (bovine plasma) 5 x 20 µg ¥3,150.00
FNR02-B Fibronectin HiLyte488 (bovine plasma) 20 x 20 µg ¥9,100.00
FNR03-A Fibronectin Biotin (bovine plasma) 5 x 20 µg ¥3,150.00
FNR03-B Fibronectin Biotin (bovine plasma) 20 x 20 µg ¥9,100.00
FTZ01-A FtsZ Protein (E.coli recomb. no tag) 1 x 1 mg ¥4,130.00
FTZ01-B FtsZ Protein (E.coli recomb. no tag) 5 x 1 mg ¥17,500.00
FTZ02-A FtsZ Protein (Staphylococcus aureus recombinant plus 6xHis tag) 1 x 1 mg ¥4,130.00
FTZ02-B FtsZ Protein (Staphylococcus aureus recombinant plus 6xHis tag) 5 x 1 mg ¥17,500.00
FTZ02-DX FtsZ Protein (Staphylococcus aureus recombinant plus 6xHis tag) 1 x 10 mg ¥24,500.00
FTZ03-A FtsZ Protein (Streptococcus pneumoniae recombinant plus 6xHis tag) 1 x 1 mg ¥4,130.00
FTZ03-B FtsZ Protein (Streptococcus pneumoniae recombinant plus 6xHis tag) 5 x 1 mg ¥17,500.00
FTZ03-DX FtsZ Protein (Streptococcus pneumoniae recombinant plus 6xHis tag) 1 x 10 mg ¥24,500.00
FTZ04-A FtsZ Protein (Enterococcus faecalis recombinant plus 6xHis tag) 1 x 1 mg ¥4,130.00
FTZ04-B FtsZ Protein (Enterococcus faecalis recombinant plus 6xHis tag) 5 x 1 mg ¥17,500.00
FTZ04-DX FtsZ Protein (Enterococcus faecalis recombinant plus 6xHis tag) 1 x 10 mg ¥24,500.00
FTZ05-A FtsZ Protein (E.coli recombinant, 6xHis-tagged) 1 x 1 mg ¥4,130.00
FTZ05-B FtsZ Protein (E.coli recombinant, 6xHis-tagged) 5 x 1 mg ¥17,500.00
FTZ05-DX FtsZ Protein (E.coli recombinant, 6xHis-tagged) 1 x 10 mg ¥24,500.00
GAP01-A Rho GAP Protein GST 1 x 50 µg ¥3,150.00
GAP01-B Rho GAP Protein GST 4 x 50 µg ¥8,190.00
GAS01-A Rho GAP Protein: catalytic domain GST 1 x 50 µg ¥3,150.00
GAS01-B Rho GAP Protein: catalytic domain GST 4 x 50 µg ¥8,190.00
GDI01-A GDP dissociation inhibitor protein GST fusion 1 x 25 µg ¥3,150.00
GDI01-B GDP dissociation inhibitor protein GST fusion 4 x 25 µg ¥8,190.00
GE01-A Dbs protein GEF domain – HIS fusion 2 x 50 µg ¥3,150.00
GE01-B Dbs protein GEF domain – HIS fusion 8 x 50 µg ¥8,190.00
H001-B Hela Cancer Cell Tubulin 1 x 250 µg ¥9,100.00
H003 Hela Cancer Cell Tubulin Biotinylated 1 x 40 µg ¥7,700.00
H005 MCF-7 Cancer Cell Tubulin 1 x 250 µg ¥9,100.00
HPG6-A Gelsolin (recomb. Human) 4 x 20 µg ¥3,570.00
HPG6-B Gelsolin (recomb. Human) 20 x 20 µg ¥11,550.00
HTS02-A Tubulin (HTS format, bovine, 8 assays) 1 x 4 mg ¥3,500.00
HTS02-B Tubulin (HTS format, bovine, 96 assays) 1 x 40 mg ¥18,900.00
HTS02-XL Tubulin (HTS format, bovine, 250 assays) 1 x 100 mg ¥37,100.00
HTS03-A Tubulin (HTS format, porcine, 8 assays) 1 x 4 mg ¥3,500.00
HTS03-B Tubulin (HTS format, porcine, 96 assays) 1 x 40 mg ¥18,900.00
HTS03-XL Tubulin (HTS format, porcine, 250 assays) 1 x 100 mg ¥37,100.00
KC01-A KIFC3 Motor Domain (Hm.sap) 2 x 25 µg ¥4,186.00
KC01-B KIFC3 Motor Domain (Hm.sap) 10 x 25 µg ¥13,930.00
KF01-A KIF3C Motor Domain (Hm.sap.) 2 x 25 µg ¥4,186.00
KF01-B KIF3C Motor Domain (Hm.sap.) 10 x 25 µg ¥13,930.00
KF01-XL KIF3C Motor Domain (Hm.sap.) (Bulk discounts avail.) 1 x 1 mg ¥23,730.00
KR01-A KHC Motor Domain (Hm.sap.) 2 x 25 µg ¥4,186.00
KR01-B KHC Motor Domain (Hm.sap.) 10 x 25 µg ¥13,930.00
KR01-XL KHC Motor Domain (Hm.sap.) (Bulk discounts avail.) 1 x 1 mg ¥23,730.00
LMN01-A Laminin Rhodamine (mouse tumor) 5 x 20 µg ¥3,150.00
LMN01-B Laminin Rhodamine (mouse tumor) 20 x 20 µg ¥9,100.00
LMN02-A Laminin HiLyte488 (mouse tumor) 5 x 20 µg ¥3,150.00
LMN02-B Laminin HiLyte488 (mouse tumor) 20 x 20 µg ¥9,100.00
LMN03-A Laminin Biotin (mouse tumor) 5 x 20 µg ¥3,150.00
LMN03-B Laminin Biotin (mouse tumor) 20 x 20 µg ¥9,100.00
MAPF-A MAP Fraction 1 x 100 µg ¥2,590.00
MAPF-C MAP Fraction 5 x 100 µg ¥9,310.00
MH01-A Myosin: Heavy Meromyosin 4 x 50 µg ¥3,150.00
MH01-B Myosin: Heavy Meromyosin 20 x 50 µg ¥9,100.00
MK01-A MCAK Motor Domain (Hm.sap.) 2 x 25 µg ¥4,186.00
MK01-B MCAK Motor Domain (Hm.sap.) 10 x 25 µg ¥13,930.00
MK01-XL MCAK Motor Domain (Hm.sap.) (Bulk discount avail.) 1 x 1 mg ¥23,730.00
ML116-A Tubulin: MAP-rich (porcine) 1 x 1 mg ¥1,386.00
ML116-B Tubulin: MAP-rich (porcine) 5 x 1 mg ¥5,530.00
ML116-C Tubulin: MAP-rich (porcine) 20 x 1 mg ¥15,750.00
ML116-DX Tubulin: MAP-rich (porcine) 1 x 10 mg ¥8,750.00
ML116-XL Tubulin: MAP-rich (procine) Large quantities 请询价
MP01-A MKLP Motor Domain (Hm.sap.) 2 x 25 µg ¥4,186.00
MP01-B MKLP Motor Domain (Hm.sap.) 10 x 25 µg ¥13,930.00
MP01-XL MKLP Motor Domain (Hm.sap.) (Bulk discount avail.) 1 x 1 mg ¥23,730.00
MT001-A Microtubules Pre-formed Motor ATPase ready 4 x 500 µg ¥4,130.00
MT001-XL Microtubules Pre-formed Motor ATPase ready 1 x 10 mg ¥15,330.00
MT002-A Microtubules Pre-formed Motor ATPase ready (porcine) 4 x 500 µg ¥4,130.00
MT002-XL Microtubules Pre-formed Motor ATPase ready (porcine) 1 x 10 mg ¥15,330.00
MY02-A Myosin Skeletal Muscle 5 x 1 mg ¥2,450.00
MY02-B Myosin Skeletal Muscle 20 x 1 mg ¥9,100.00
MY03-A Myosin Cardiac Muscle 1 x 1 mg ¥2,450.00
MY03-B Myosin Cardiac Muscle 5 x 1 mg ¥9,100.00
PAK01-A PAK Rac1/Cdc42 binding domain protein 1 x 250 µg ¥4,410.00
PAK01-B PAK Rac1/Cdc42 binding domain protein 4 x 250 µg ¥12,530.00
PAK02-A PAK Beads, Rac1/Cdc42 binding domain protein on beads 1 x 500 µg ¥5,390.00
PAK02-B PAK Beads, Rac1/Cdc42 binding domain protein on beads 4 x 500 µg ¥16,100.00
PHDG1-A Acti-stain™ 488 phalloidin 1 x 500 µl (300 slides) ¥1,750.00
PHDH1-A Acti-stain™ 555 phalloidin 1 x 500 µl (300 slides) ¥1,750.00
PHDN1-A Acti-stain™ 670 phalloidin 1 x 500 µl (300 slides) ¥1,750.00
PHDR1 Rhodamine Phalloidin (14uM in methanol) 1 x 500 µl ¥1,386.00
PIC02 Protease Inhibitor Cocktail (100x strength) 1 x 1 ml ¥560.00
PR01-A Profilin 1 (recombinant human) 1 x 50 µg ¥3,150.00
PR01-C Profilin 1 (recombinant human) 4 x 50 µg ¥9,100.00
R17G01-A Rac1 GST Protein: dominant negative 1 x 25 µg ¥3,150.00
R17G01-C Rac1 GST Protein: dominant negative 4 x 25 µg ¥7,700.00
R6101-A Rac1 His Protein: constitutively active 1 x 10 µg ¥3,150.00
R6101-C Rac1 His Protein: constitutively active 4 x 10 µg ¥7,700.00
R6301-A RhoA His Protein: constitutively active 1 x 10 µg ¥3,150.00
R6301-C RhoA His Protein: constitutively active 4 x 10 µg ¥7,700.00
RC01-A Rac1 His Protein: wild type 1 x 100 µg ¥3,150.00
RC01-C Rac1 His Protein: wild type 3 x 100 µg ¥7,700.00
RC01-XL Rac1 His Protein: wild type 1 x 1 mg ¥17,500.00
RC02-A Rac2 His Protein: wild type 1 x 100 µg ¥3,150.00
RC02-B Rac2 His Protein: wild type 3 x 100 µg ¥7,700.00
RCG01-C Rac1 GST Protein: wild type 8 x 25 µg ¥3,710.00
RF02-A Raf protein ras binding domain on GST beads 1 x 2 mg ¥5,390.00
RF02-B Raf protein ras binding domain on GST beads 4 x 2 mg ¥16,100.00
RH01-A RhoA His Protein: wild type 1 x 100 µg ¥3,150.00
RH01-C RhoA His Protein: wild type 3 x 100 µg ¥7,700.00
RH01-XL RhoA His Protein: wild type 1 x 1 mg ¥17,500.00
RH03-A RhoC His Protein: wild type 1 x 100 µg ¥3,150.00
RH03-C RhoC His Protein: wild type 3 x 100 µg ¥7,700.00
RHG01-C RhoA GST Protein: wild type 8 x 25 µg ¥5,250.00
RN01-A Ran protein (Human wild type, His) 1 x 10 µg ¥3,150.00
RN01-B Ran protein (Human wild type, His) 3 x 10 µg ¥7,700.00
RN03-A Ran protein (Human Q69L mutant, His) 1 x 10 µg ¥3,150.00
RN03-B Ran protein (Human Q69L mutant, His) 3 x 10 µg ¥7,700.00
RN05-A Ran protein (Human T24N mutant, His) 1 x 10 µg ¥3,150.00
RN05-B Ran protein (Human T24N mutant, His) 3 x 10 µg ¥7,700.00
RN07-A Ran BP1 protein (Xenopus wild type, His) 1 x 250 µg ¥3,850.00
RN07-B Ran BP1 protein (Xenopus wild type, His) 3 x 250 µg ¥8,750.00
RP01-A Arp2/3 Protein Complex (Bovine brain) 2 x 50 µg ¥5,250.00
RP01-B Arp2/3 Protein Complex (Bovine brain) 6 x 50 µg ¥13,650.00
RR02-A Rap1b protein (Human wild type, His) 1 x 100 µg ¥3,150.00
RR02-B Rap1b protein (Human wild type, His) 3 x 100 µg ¥7,700.00
RS01-A H-Ras-His Protein wild type 1 x 100 µg ¥3,150.00
RS01-C H-Ras-His Protein wild type 3 x 100 µg ¥7,700.00
RT01-A Rhotekin RBD protein GST fusion 1 x 500 µg ¥5,250.00
RT01-B Rhotekin RBD protein GST fusion 3 x 500 µg ¥13,300.00
RT02-A Rhotekin RBD protein on GST beads 2 x 2 mg ¥6,300.00
RT02-B Rhotekin RBD protein on GST beads 6 x 2 mg ¥15,330.00
T238P-A Tubulin (porcine brain, >99% pure) 1 x 1 mg ¥2,100.00
T238P-B Tubulin (porcine brain, >99% pure) 5 x 1 mg ¥8,330.00
T238P-C Tubulin (porcine brain, >99% pure) 20 x 1 mg ¥23,730.00
T240-A Tubulin (porcine brain, >99% pure) 1 x 1 mg ¥1,540.00
T240-B Tubulin (porcine brain, >99% pure) 5 x 1 mg ¥6,930.00
T240-C Tubulin (porcine brain, >99% pure) 20 x 1 mg ¥23,590.00
T240-DX Tubulin (porcine brain, >99% pure) 1 x 10 mg ¥11,550.00
T240-XL Tubulin (porcine brain, >99% pure) Large quantities 请询价
T333P-A Tubulin (Biotin Conjugate; Porcine Brain) 5 x 20 µg ¥3,570.00
T333P-B Tubulin (Biotin Conjugate; Porcine Brain) 20 x 20 µg ¥9,730.00
T333P-XL Tubulin (Biotin Conjugate; Porcine Brain) 1 x 500 µg ¥8,330.00
TA01-A Tau (bovine brain) 1 x 50 µg ¥3,430.00
TA01-B Tau (bovine brain) 3 x 50 µg ¥8,050.00
TL238-A Tubulin (bovine brain, >99% pure, lyophilized) 4 x 250 µg ¥2,310.00
TL238-B Tubulin (bovine brain, >99% pure, lyophilized) 1 x 1 mg ¥1,540.00
TL238-C Tubulin (bovine brain, >99% pure, lyophilized) 5 x 1 mg ¥6,930.00
TL238-D Tubulin (bovine brain, >99% pure, lyophilized) 10 x 1 mg ¥12,530.00
TL238-DX Tubulin (bovine brain, >99% pure, lyophilized) 1 x 10 mg ¥11,550.00
TL238-E Tubulin (bovine brain, >99% pure, lyophilized) 20 x 1 mg ¥23,590.00
TL238-F Tubulin (bovine brain, >99% pure, lyophilized) Large quantities 请询价
TL440M-A Tubulin (AMCA dye labeled; porcine, micro-injctn) 5 x 20 µg ¥3,500.00
TL440M-B Tubulin (AMCA dye labeled; porcine, micro-injctn) 20 x 20 µg ¥8,890.00
TL488M-A Tubulin (HiLyte 488TM dye labeled; porcine, micro-injctn) 5 x 20 µg ¥3,500.00
TL488M-B Tubulin (HiLyte 488TM dye labeled; porcine, micro-injctn) 20 x 20 µg ¥8,890.00
TL590M-A Tubulin (Rhodamine labeled; porcine, micro-injctn) 5 x 20 µg ¥3,500.00
TL590M-B Tubulin (Rhodamine labeled; porcine, micro-injctn) 20 x 20 µg ¥8,890.00
TL620M-A Tubulin (X-rhodamine labeled; Bovine Brain micr-injctn) 5 x 20 µg ¥3,500.00
TL620M-B Tubulin (X-rhodamine labeled; Bovine Brain micr-injctn) 20 x 20 µg ¥8,890.00
TL670M-A Tubulin (HiLyte 647TM dye labeled; porcine, micro-injctn) 5 x 20 µg ¥3,500.00
TL670M-B Tubulin (HiLyte 647TM dye labeled; porcine, micro-injctn) 20 x 20 µg ¥8,890.00
TP005 Tubulin protein Soybean plant 1 x 250 µg ¥10,990.00
TXD01 Paclitaxel (2mM in DMSO) 10 x 100 µl ¥2,100.00
V01-A Vimentin (Recombinant Syrian Hamster) 2 x 50 µg ¥3,990.00
V01-C Vimentin (Recombinant Syrian Hamster) 10 x 50 µg ¥14,350.00
VCG03-A VCA domain of WASP (GST fusion) 1 x 500 µg ¥5,110.00
VCG03-B VCA domain of WASP (GST fusion) 5 x 500 µg ¥17,500.00

Cytoskeleton 2016年价目表

美国Cytoskeleton公司自1993年成立以来,不断扩大产品线,为药物开发、信号转导和细胞骨架方面的研究提供高质量的产品。该公司专著于生产纯化蛋白和使用方便的试剂盒,用于研究生化和细胞信号过程。G-LISATM系列试剂盒用ELISA为基础的方法在3小时之内快捷准确地测定小G蛋白的活性。

从1993年开始,Cytoskeleton公司就为广大科研工作者提供纯化的tubulin蛋白以及相关试剂盒。Cytoskeleton公司一直致力于提供纯度更高、活性跟高的tubulin蛋白及相关试剂和试剂盒,包括真菌来源的、人来源的蛋白;荧光标记、生物素标记的蛋白。

 

 纯度*

 多种来源

● 标记和不标记

● 被很多文献引用

● 20年的历史

 

部分产品报价:

厂家 货号 品名 规格 人民币目录价
Cytoskeleton AAC01 Anti-Acetyl Lysine Mouse Monoclonal Antibody. 2 x 100 µl 4,149.60
Cytoskeleton AAC01-S Anti-Acetyl Lysine Mouse Monoclonal Antibody (Trial size) 1 x 25 µl 1,092.00
Cytoskeleton AAC01-beads Anti-Acetyl Lysine Affinity Beads 2 x 200 µl 5,096.00
Cytoskeleton AAN01-A Anti-actin antibody (rabbit polyAb) + control 1 x 100 µg 3,494.40
Cytoskeleton AAN01-B Anti-actin antibody (rabbit polyAb) + control 3 x 100 µg 8,372.00
Cytoskeleton AB07-A Actin protein (biotin, rabbit skeletal muscle) 5 x 20 µg 3,931.20
Cytoskeleton AB07-C Actin protein (biotin, rabbit skeletal muscle) 20 x 20 µg 10,992.80
Cytoskeleton ACD03 Anti-Cdc42 Mouse Monoclonal Antibody  2 x 200 µl 4,149.60
Cytoskeleton ACD03-S Anti-Cdc42 Mouse Monoclonal Antibody (sample size)  1 x 50 µl 1,092.00
Cytoskeleton ACFL02-A Cofilin antibody (host rabbit) + control 1 x 50 µg 3,931.20
Cytoskeleton ACFL02-B Cofilin antibody (host rabbit) + control 3 x 50 µg 9,900.80
Cytoskeleton AD99-A Actin protein (bovine cardiac muscle, >99% pure) 1 x 1 mg 1,441.44
Cytoskeleton AD99-B Actin protein (bovine cardiac muscle, >99% pure) 5 x 1 mg 6,552.00
Cytoskeleton ADV01-A Advanced Protein Assay (5X conc) 1 x 500 ml 2,693.60
Cytoskeleton ADV01-B Advanced Protein Assay (5X conc) 3 x 500 ml 7,134.40
Cytoskeleton ADV02-A Precision Red Advanced Protein Assay (1X conc) 1 x 500 ml 1,718.08
Cytoskeleton ADV02-B Precision Red Advanced Protein Assay (1X conc) 3 x 500 ml 4,732.00
Cytoskeleton AKF99-A Pre-formed actin filaments (rabbit skeletal muscle) 1 x 1 mg 2,402.40
Cytoskeleton AKF99-B Pre-formed actin filaments (rabbit skeletal muscle) 5 x 1 mg 8,372.00
Cytoskeleton AKL95-B Actin protein (rabbit skeletal muscle, >95% pure) 1 x 1  mg 1,237.60
Cytoskeleton AKL95-C Actin protein (rabbit skeletal muscle, >95% pure) 5 x 1  mg 4,732.00
Cytoskeleton AKL99-A Actin protein (rabbit skeletal muscle, >99% pure) 4 x 250 µg 2,548.00
Cytoskeleton AKL99-B Actin protein (rabbit skeletal muscle, >99% pure) 2 x 1 mg 3,203.20
Cytoskeleton AKL99-C Actin protein (rabbit skeletal muscle, >99% pure) 5 x 1 mg 6,406.40
Cytoskeleton AKL99-D Actin protein (rabbit skeletal muscle, >99% pure) 10 x 1 mg 11,575.20
Cytoskeleton AKL99-E Actin protein (rabbit skeletal muscle, >99% pure) 20 x 1 mg 19,874.40
Cytoskeleton AP05-A Actin protein (pyrene, rabbit skeletal muscle) 1 x 1 mg 2,839.20
Cytoskeleton AP05-B Actin protein (pyrene, rabbit skeletal muscle) 5 x 1 mg 11,138.40
Cytoskeleton APHL99-A Actin protein (human plaet non-muscle, >99% pure) 2 x 250 µg 2,693.60
Cytoskeleton APHL99-C Actin protein (human plaet non-muscle, >99% pure) 1 x 1 mg 3,785.60
Cytoskeleton APHL99-E Actin protein (human plaet non-muscle, >99% pure) 5 x 1 mg 13,832.00
Cytoskeleton APHR-A Actin protein (rhodamine, human plaet non-muscle) 4 x 10 µg 3,858.40
Cytoskeleton APHR-C Actin protein (rhodamine, human plaet non-muscle) 20 x 10 µg 11,575.20
Cytoskeleton APUF01-A Profilin polyclonal antibody (host rabbit) + control 1 x 50 µg 2,839.20
Cytoskeleton APY03-S Anti-Phosphotyrosine Mouse Monoclonal Antibody (Trial Size) 1 x 25 µl 1,092.00
Cytoskeleton APY03 Anti-Phosphotyrosine Mouse Monoclonal Antibody 2 x 100 µl 4,149.60
Cytoskeleton APY03-HRP-S Anti-Phosphotyrosine-HRP Mouse Monoclonal Antibody (Trial Size) 1 x 25 µl 1,383.20
Cytoskeleton APY03-HRP Anti-Phosphotyrosine-HRP Mouse Monoclonal Antibody 1 x 100 µl 5,096.00
Cytoskeleton APY03-Beads Anti-Phosphotyrosine Affinity Beads 4 x 300 µl 5,096.00
Cytoskeleton AR05-B Actin protein (rhodamine, rabbit skeletal muscle) 10 x 20 µg 3,640.00
Cytoskeleton AR05-C Actin protein (rhodamine, rabbit skeletal muscle) 20 x 20 µg 7,134.40
Cytoskeleton ARC03 Anti-Rac1 specific mouse MAb 2 x 100 µl 4,149.60
Cytoskeleton ARC03-S Anti-Rac1 specific mouse MAb(sample size) 1 x 25 µl 1,092.00
Cytoskeleton ARH03-A Anti-RhoA antibody (mouse MAb) + control. 2 x 50 µg 3,494.40
Cytoskeleton ARH03-B Anti-RhoA antibody (mouse MAb) + control. 6 x 50 µg 7,571.20
Cytoskeleton ARH04 Anti-RhoA antibody (mouse MAb) + control. 2 x 100 µl 4,149.60
Cytoskeleton ARH04-S Anti-RhoA antibody (mouse MAb) + control.  (Trial size) 1 x 50 µl 1,092.00
Cytoskeleton AS99-A Actin protein (chicken gizzard muscle, >99% pure) 1 x 1 mg 1,441.44
Cytoskeleton AS99-B Actin protein (chicken gizzard muscle, >99% pure) 5 x 1 mg 6,552.00
Cytoskeleton ASM24-Beads SUMO-2/3 Affinity Beads 2 x 400 µl 5,096.00
Cytoskeleton ASM23-S Anti-SUMO-2/3 Mouse Monoclonal Antibody (Trial Size) 1 x 25 µl 1,092.00
Cytoskeleton ASM23 Anti-SUMO-2/3 Mouse Monoclonal Antibody 2 x 100 µl 4,149.60
Cytoskeleton ASM24-S Anti-SUMO-2/3 Mouse Monoclonal Antibody (Trial Size) 1 x 150 µl 1,092.00
Cytoskeleton ASM24 Anti-SUMO-2/3 Mouse Monoclonal Antibody 2 x 200 µl 4,149.60
Cytoskeleton AT01-A Alpha-actinin (rabbit skeletal muscle) 2 x 50 µg 3,712.80
Cytoskeleton AT01-C Alpha-actinin (rabbit skeletal muscle) 10 x 50 µg 12,448.80
Cytoskeleton ATN02 Anti-tubulin polyAb pan specific (sheep)  2 x 100 µl 4,149.60
Cytoskeleton ATN02-S Anti-tubulin polyAb pan specific (sheep) (Trial size) 1 x 25 µl 1,092.00
Cytoskeleton AUB01-S Anti-Ubiquitin Mouse Monoclonal Antibody (Trial size) 1 x 25 µl 1,092.00
Cytoskeleton AUB01 Anti-Ubiquitin Mouse Monoclonal Antibody 2 x 100 µl 4,149.60
Cytoskeleton AUB01-XL Anti-Ubiquitin Mouse Monoclonal Antibody 4 x 500 µl 9,464.00
Cytoskeleton BK001 Actin Binding Protein Spin-Down Assay Kit KIT 30-100 assays 9,755.20
Cytoskeleton BK003 Actin Polymerization Biochem Kit  KIT 30-100 assays 10,920.00
Cytoskeleton BK004P Tubulin Polymerization Assay Kit (>97% pure porcine brain tubulin) KIT 24 assays 8,663.20
Cytoskeleton BK005 F-Actin Visualization Kit KIT 300 slides 5,023.20
Cytoskeleton BK006P Tubulin Polymerization Assay Kit (>99% pure porcine brain tubulin) KIT 24 assays 12,667.20
Cytoskeleton BK008 Ras Activation Assay Biochem Kit KIT 50 assays 10,046.40
Cytoskeleton BK008-S Ras Activation Assay Biochem Kit KIT 20 assays 5,023.20
Cytoskeleton BK011P Tubulin Polymerization Assay (fluoresc-96assays porcine brain tubulin) KIT 96 assays 12,667.20
Cytoskeleton BK013 Actin Binding Protein Spin-Down Assay Kit (Non-muscle) KIT 30-100 assays 10,119.20
Cytoskeleton BK029 Microtubule Binding Protein Spin Down Assay Kit KIT 30-100 assays 9,391.20
Cytoskeleton BK030 Combo RhoA/Rac1/Cdc42 Activation Assay Kit KIT 3 x 10 assays 12,230.40
Cytoskeleton BK032-S Arf1 Pulldown Activation Assay Kit KIT 20 assays 5,751.20
Cytoskeleton BK033-S Arf6 Pulldown Activation Assay Kit KIT 20 assays 5,751.20
Cytoskeleton BK034 Cdc42 Pulldown Activation Assay Kit KIT 50 assays 10,046.40
Cytoskeleton BK034-S Cdc42 Pulldown Activation Assay Kit KIT 20 assays 5,023.20
Cytoskeleton BK035 Rac1 Pulldown Activation Assay Kit KIT 50 assays 10,046.40
Cytoskeleton BK035-S Rac1 Pulldown Activation Assay Kit KIT 20 assays 5,023.20
Cytoskeleton BK036 RhoA Pulldown Activation Assay Kit KIT 80 assays 10,046.40
Cytoskeleton BK036-S RhoA Pulldown Activation Assay Kit KIT 20 assays 5,023.20
Cytoskeleton BK037 G-Actin : F-Actin In Vivo Assay Kit KIT 30-100 assays 10,119.20
Cytoskeleton BK038 Microtubule / Tubulin In Vivo Assay Kit KIT 30-100 assays 10,119.20
Cytoskeleton BK040 RalA Activation Assay Biochem Kit KIT 50  assays 10,046.40
Cytoskeleton BK051/52 ATPase ELIPA™ (Enzyme Linked Inorganic Phosphate Assay) KIT 96 assays 4,295.20
Cytoskeleton BK051/52 GTPase ELIPA™ (Enzyme Linked Inorganic Phosphate Assay) KIT 96 assays 4,295.20
Cytoskeleton BK053 HTS Kinesin ATPase Endpoint Assay KIT 1000 assays 10,920.00
Cytoskeleton BK054 CytoPhos™ Phosphate Assay (1-500ug/ml protein reactions) KIT 1000 assays 4,295.20
Cytoskeleton BK060 Kinesin ELIPA (Enzyme Linked Inorganic Phosphate Assay TM) KIT 96 assays 10,556.00
Cytoskeleton BK100 GEF Exchange Assay KIT 60-300 assays 9,100.00
Cytoskeleton BK105 GAP Assay Kit KIT 80-160 assays 9,100.00
Cytoskeleton BK121 RhoA G-LISA Activation Assay (luminescence) KIT 96 assays 12,376.00
Cytoskeleton BK124-S RhoA G-LISA Activation Assay (absorbance) KIT 24 assays 7,207.20
Cytoskeleton BK124 RhoA G-LISA Activation Assay (absorbance) KIT 96 assays 12,376.00
Cytoskeleton BK125 Rac1,2,3 G-LISA Activation Assay Kit KIT 96 assays 12,376.00
Cytoskeleton BK126 Rac1 G-LISA Activation Assay Kit KIT 96 assays 12,376.00
Cytoskeleton BK127 Cdc42 G-LISA Activation Assay Kit KIT 96 assays 12,376.00
Cytoskeleton BK127-S Cdc42 G-LISA Activation Assay Kit KIT 24 assays 7,207.20
Cytoskeleton BK128 Rac1 G-LISA Activation Assay Kit KIT 96 assays 12,376.00
Cytoskeleton BK128-S Rac1 G-LISA Activation Assay Kit KIT 24 assays 7,207.20
Cytoskeleton BK129 RalA G-LISA Activation Assay Kit KIT 96 assays 12,376.00
Cytoskeleton BK131 Ras G-LISA Activation Assay Kit KIT 96 assays 12,376.00
Cytoskeleton BK132 Arf1 G-LISA Activation Assay Kit KIT 96 assays 12,376.00
Cytoskeleton BK133 Arf6 G-LISA Activation Assay Kit KIT 96 assays 12,376.00
Cytoskeleton BK135 G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S) 3 KITS (24 assays/kit) 19,656.00
Cytoskeleton BK150 RhoA ELISA Kit KIT 96 assays 6,552.00
Cytoskeleton BM01-A BimC Motor Domain (Asp.nid.) 2 x 25 µg 4,513.60
Cytoskeleton BS01 GTP gamma S 1 x 500 µg 1,310.40
Cytoskeleton BSA01-001 General Actin Buffer 1 x 10 ml 960.96
Cytoskeleton BSA01-010 General Actin Buffer 1 x 100 ml 2,111.20
Cytoskeleton BSA02-001 Actin Polymerization Buffer (10x stock) 1 x 2 ml 960.96
Cytoskeleton BSA04-001 ATP (100mM Stock) 1 x 1 ml 960.96
Cytoskeleton BST01-001 General Tubulin Buffer 1 x 10 ml 960.96
Cytoskeleton BST01-010 General Tubulin Buffer 1 x 100 ml 2,111.20
Cytoskeleton BST05-001 Tubulin Glycerol Buffer 1 x 10 ml 960.96
Cytoskeleton BST06-001 GTP (100mM Stock) 1 x 100 µl 960.96
Cytoskeleton BST06-010 GTP (100mM Stock) 10 x 100 µl 2,839.20
Cytoskeleton C17G01-A Cdc42-GST protein: dominant negative 1 x 25 µg  3,640.00
Cytoskeleton C6101-A Cdc42-His Protein: constituitively active 1 x 10 µg 3,640.00
Cytoskeleton CD01-A Cdc42-His Protein: Wild type 1 x 100 µg 3,640.00
Cytoskeleton CD01-C Cdc42-His Protein: Wild type 3 x 100 µg 8,881.60
Cytoskeleton CD01-XL Cdc42-His Protein: Wild type 1 x 1 mg 18,782.40
Cytoskeleton CDG01-C Cdc42-GST Protein: Wild type 8 x 25 µg 6,814.08
Cytoskeleton CF01-A Cofilin (Human Recombinant) 1 x 100 µg 3,421.60
Cytoskeleton CF01-C Cofilin (Human Recombinant) 4 x 100 µg 10,992.80
Cytoskeleton CN01-A Rho Activator 5 x 10 units 2,868.32
Cytoskeleton CN01-B Rho Activator 20 x 10 units 10,119.20
Cytoskeleton CN02-A Rac and Cdc42 Activator 5 x 10 units 2,868.32
Cytoskeleton CN02-B Rac and Cdc42 Activator 20 x 10 units 10,119.20
Cytoskeleton CN03-A Rho activator II 3 x 20 µg 2,868.32
Cytoskeleton CN03-B Rho activator II 9 x 20 µg 8,153.60
Cytoskeleton CN04-A Rho/Rac/Cdc42 Activator I 3 x 20 µg 2,868.32
Cytoskeleton CN04-B Rho/Rac/Cdc42 Activator I 9 x 20 µg 8,153.60
Cytoskeleton CP01-A CENP-E Motor domain (Hm.sap.) 2 x 25 µg 4,732.00
Cytoskeleton CP01-XL CENP-E Motor domain (Hm.sap.) (Bulk discount avail.) 1 x 1 mg 24,024.00
Cytoskeleton CR01-A Chromokinesin Motor Domain (Hm.sap.) 2 x 25 µg 4,732.00
Cytoskeleton CS-DN01 Dynein (Cytoplasmic), Porcine brain 1 x 50 µg 5,678.40
Cytoskeleton CS-H001-B Hela Cancer Cell Tubulin 1 x 250 µg 11,284.00
Cytoskeleton CS-H005 MCF-7 Cancer Cell Tubulin 1 x 250 µg 11,284.00
Cytoskeleton CS-KF51 KIF7 motor domain (human recombinant) 1 x 100 µg 4,732.00
Cytoskeleton CS-MH03 Heavy Meromyosin Protein (HMM Fragment) 1 x 100 µg 4,295.20
Cytoskeleton CS-MP05 MKLP2 motor domain (human recombinant) 1 x 50 µg 5,460.00
Cytoskeleton CS-MYS03 Myosin S1 Fragment (Cardiac), Bovine cardiac 1 x 250 µg 3,640.00
Cytoskeleton CS-MYS04 Myosin S1 Fragment (Skeletal), Rabbit skeletal 1 x 250 µg 3,640.00
Cytoskeleton CS-MYS05 Myosin S1 Fragment (smooth), Chicken gizzard 1 x 250 µg 3,640.00
Cytoskeleton CS-RS03 K-Ras4B Protein: wild-type (human recombinant) 1 x 100 µg 5,314.40
Cytoskeleton CS-SOS1-A SOS1 Ras GEF protein 1 x 100 µg 5,314.40
Cytoskeleton CS-SOS1-B SOS1 Ras GEF protein 1 x 1 mg 24,024.00
Cytoskeleton CS-T234S Sheep brain tubulin 1 x 5 mg 8,008.00
Cytoskeleton CS-TFC01 Actin Thin Filaments (Bovine cardiac) 1 x 1 mg 8,736.00
Cytoskeleton CS-TT05 Tropomyosin / Tropomodulin Complex, Bovine cardiac 1 x 1 mg 3,640.00
Cytoskeleton CT03-A C3 transferase protein 1 x 25 µg 2,868.32
Cytoskeleton CT03-C C3 transferase protein 4 x 25 µg 9,973.60
Cytoskeleton CT04-A Cell Permeable Rho Inhibitor (C3 Trans based)) 1 x 20 µg 2,868.32
Cytoskeleton CT04-B Cell Permeable Rho Inhibitor (C3 Trans based)) 5 x 20 µg 8,517.60
Cytoskeleton CT04-C Cell Permeable Rho Inhibitor (C3 Trans based)) 20 x 20 µg 22,568.00
Cytoskeleton CY-SC001 SiR-Actin Kit 50-300 slides 7,265.44
Cytoskeleton CY-SC002 SiR-Tubulin Kit 50-300 slides 7,265.44
Cytoskeleton CY-SC006 Cytoskeleton Kit (SiR-Actin + SiR-Tubulin) 50-300 slides each 12,376.00
Cytoskeleton CY-SC007 SiR-DNA Kit 50-300 slides 4,295.20
Cytoskeleton EG01-A Eg5 Motor Domain (Hm.sap.) 2 x 25 µg 4,732.00
Cytoskeleton EG01-B Eg5 Motor Domain (Hm.sap.) 10 x 25 µg 14,924.00
Cytoskeleton EG01-XL Eg5 Motor Domain (Hm.sap.) (Bulk discount avail.) 1 x 1 mg 24,024.00
Cytoskeleton EG02-A Eg5 Homolog Motor Domain (Asp.fum.) 2 x 15 µg 4,513.60
Cytoskeleton ELP03 Purine Nucleoside Phosphorylase (PNP) 1 x 50 U 2,839.20
Cytoskeleton FNR01-A Fibronectin Rhodamine (bovine plasma) 5 x 20 µg 4,295.20
Cytoskeleton FNR01-B Fibronectin Rhodamine (bovine plasma) 20 x 20 µg 12,376.00
Cytoskeleton FNR02-A Fibronectin HiLyte488™ (bovine plasma) 5 x 20 µg 4,295.20
Cytoskeleton FNR02-B Fibronectin HiLyte488™ (bovine plasma) 20 x 20 µg 12,376.00
Cytoskeleton FNR03-A Fibronectin Biotin (bovine plasma) 5 x 20 µg 4,295.20
Cytoskeleton FNR03-B Fibronectin Biotin (bovine plasma) 20 x 20 µg 12,376.00
Cytoskeleton FTZ02-A FtsZ Protein (Staphylococcus aureus recombinant plus 6xHis tag) 1 x 1 mg 5,241.60
Cytoskeleton FTZ02-B FtsZ Protein (Staphylococcus aureus recombinant plus 6xHis tag) 5 x 1 mg 23,296.00
Cytoskeleton FTZ03-A FtsZ Protein (Streptococcus pneumoniae recombinant plus 6xHis tag) 1 x 1 mg 5,241.60
Cytoskeleton FTZ03-B FtsZ Protein (Streptococcus pneumoniae recombinant plus 6xHis tag) 5 x 1 mg 23,296.00
Cytoskeleton FTZ04-A FtsZ Protein (Enterococcus faecalis recombinant plus 6xHis tag) 1 x 1 mg 5,241.60
Cytoskeleton FTZ04-B FtsZ Protein (Enterococcus faecalis recombinant plus 6xHis tag) 5 x 1 mg 23,296.00
Cytoskeleton FTZ05-A FtsZ Protein (E.coli  recombinant, 6xHis-tagged) 1 x 1 mg 5,241.60
Cytoskeleton FTZ05-B FtsZ Protein (E.coli  recombinant, 6xHis-tagged) 5 x 1 mg 23,296.00
Cytoskeleton GAP01-A Rho GAP Protein GST 1 x 50 µg 3,567.20
Cytoskeleton GAP01-B Rho GAP Protein GST 4 x 50 µg 9,391.20
Cytoskeleton GAS01-A Rho GAP Protein: catalytic domain GST 1 x 50 µg 3,567.20
Cytoskeleton GAS01-B Rho GAP Protein: catalytic domain GST 4 x 50 µg 9,391.20
Cytoskeleton GDI01-A GDP dissociation inhibitor protein GST fusion 1 x 25 µg 3,567.20
Cytoskeleton GE01-A Dbs protein GEF domain – HIS fusion 2 x 50 µg 3,567.20
Cytoskeleton GGA05-A GGA3-PBD Arf Affinity Beads 1 x 500 µg 5,751.20
Cytoskeleton H003 Hela Cancer Cell Tubulin Biotinylated 1 x 40 µg 9,100.00
Cytoskeleton HPG6-A Gelsolin (recomb. Human) 4 x 20 µg 4,149.60
Cytoskeleton HPG6-B Gelsolin (recomb. Human) 20 x 20 µg 13,832.00
Cytoskeleton HTS03-A Tubulin (HTS format, porcine, 8 assays) 1 x 4 mg 4,659.20
Cytoskeleton HTS03-B Tubulin (HTS format, porcine, 96 assays) 1 x 40 mg 22,568.00
Cytoskeleton KC01-A KIFC3 Motor Domain (Hm.sap) 2 x 25 µg 4,732.00
Cytoskeleton KF01-A KIF3C Motor Domain (Hm.sap.) 2 x 25 µg 4,732.00
Cytoskeleton KR01-A KHC Motor Domain (Hm.sap.) 2 x 25 µg 4,732.00
Cytoskeleton KR01-XL KHC Motor Domain (Hm.sap.) (Bulk discounts avail.) 1 x 1 mg 24,024.00
Cytoskeleton LMN01-A Laminin Rhodamine (mouse tumor) 5 x 20 µg 4,295.20
Cytoskeleton LMN01-B Laminin Rhodamine (mouse tumor) 20 x 20 µg 12,376.00
Cytoskeleton LMN02-A Laminin HiLyte488™ (mouse tumor) 5 x 20 µg 4,295.20
Cytoskeleton LMN02-B Laminin HiLyte488™ (mouse tumor) 20 x 20 µg 12,376.00
Cytoskeleton LMN03-A Laminin Biotin (mouse tumor) 5 x 20 µg 4,295.20
Cytoskeleton LMN03-B Laminin Biotin (mouse tumor) 20 x 20 µg 12,376.00
Cytoskeleton MAPF-A MAP Fraction 1 x 100 µg 3,057.60
Cytoskeleton MAPF-C MAP Fraction 5 x 100 µg 10,920.00
Cytoskeleton MH01-A Myosin: Heavy Meromyosin 4 x 50 µg 3,640.00
Cytoskeleton MK01-A MCAK Motor Domain (Hm.sap.) 2 x 25 µg 4,732.00
Cytoskeleton ML116-A Tubulin: MAP-rich (porcine) 1 x 1 mg 1,441.44
Cytoskeleton ML116-B Tubulin: MAP-rich (porcine) 5 x 1 mg 6,552.00
Cytoskeleton ML116-DX Tubulin: MAP-rich (porcine) 1 x 10 mg 10,920.00
Cytoskeleton MP01-A MKLP Motor Domain (Hm.sap.) 2 x 25 µg 4,732.00
Cytoskeleton MP01-XL MKLP Motor Domain (Hm.sap.)  1 x 1 mg 24,024.00
Cytoskeleton MT001-A Microtubules Pre-formed Motor ATPase ready (bovine) 4 x 500 µg 5,096.00
Cytoskeleton MT001-XL Microtubules Pre-formed Motor ATPase ready (bovine) 1 x 10 mg 17,472.00
Cytoskeleton MT002-A Microtubules Pre-formed Motor ATPase ready(porcine) 4 x 500 µg 5,096.00
Cytoskeleton MT002-XL Microtubules Pre-formed Motor ATPase ready(porcine) 1 x 10 mg 17,472.00
Cytoskeleton MY02-A Myosin Skeletal Muscle 5 x 1 mg 2,912.00
Cytoskeleton MY02-B Myosin Skeletal Muscle 20 x 1 mg 10,920.00
Cytoskeleton MY03-A Myosin Cardiac Muscle 5 x 1 mg 2,912.00
Cytoskeleton MY03-B Myosin Cardiac Muscle 20 x 1 mg 10,920.00
Cytoskeleton PAK01-A PAK Rac1/Cdc42 binding domain protein 1 x 250 µg 5,096.00
Cytoskeleton PAK01-B PAK Rac1/Cdc42 binding domain protein 4 x 250 µg 14,530.88
Cytoskeleton PAK02-A PAK Beads, Rac1/Cdc42 binding domain protein on beads 1 x 500 µg 6,042.40
Cytoskeleton PAK02-B PAK Beads, Rac1/Cdc42 binding domain protein on beads 4 x 500 µg 18,491.20
Cytoskeleton PHDG1-A Acti-stain™ 488 phalloidin 1 x 500 µl (300 slides) 2,693.60
Cytoskeleton PHDH1-A Acti-stain™ 555 phalloidin 1 x 500 µl (300 slides) 2,693.60
Cytoskeleton PHDN1-A Acti-stain™ 670 phalloidin 1 x 500 µl (300 slides) 2,693.60
Cytoskeleton PHDR1 Rhodamine Phalloidin (14uM in methanol) 1 x 500 µl 2,693.60
Cytoskeleton PIC02 Protease Inhibitor Cocktail (100x strength) 1 x 1 ml 960.96
Cytoskeleton PR01-A Profilin 1 (recombinant human) 1 x 50 µg 3,567.20
Cytoskeleton R17G01-A Rac1 GST Protein: dominant negative 1 x 25 µg 3,640.00
Cytoskeleton R6101-A Rac1 His Protein: constitutively active 1 x 10 µg 3,640.00
Cytoskeleton R6301-A RhoA His Protein: constitutively active 1 x 10 µg 3,640.00
Cytoskeleton RC01-A Rac1 His Protein: wild type 1 x 100 µg 3,640.00
Cytoskeleton RC01-C Rac1 His Protein: wild type 3 x 100 µg 9,100.00
Cytoskeleton RC01-XL Rac1 His Protein: wild type 1 x 1 mg 19,656.00
Cytoskeleton RC02-A Rac2 His Protein: wild type 1 x 100 µg 3,640.00
Cytoskeleton RCG01-C Rac1 GST Protein: wild type 8 x 25 µg 6,552.00
Cytoskeleton RF02-A Raf protein ras binding domain on GST beads 1 x 2 mg 6,406.40
Cytoskeleton RF02-B Raf protein ras binding domain on GST beads 4 x 2 mg 18,855.20
Cytoskeleton RH01-A RhoA His Protein: wild type 1 x 100 µg 3,640.00
Cytoskeleton RH01-C RhoA His Protein: wild type 3 x 100 µg 9,100.00
Cytoskeleton RH01-XL RhoA His Protein: wild type 1 x 1 mg 19,656.00
Cytoskeleton RH03-A RhoC His Protein: wild type 1 x 100 µg 3,640.00
Cytoskeleton RHG01-C RhoA GST Protein: wild type 8 x 25 µg 6,552.00
Cytoskeleton RP01P-A Arp2/3 Protein Complex (Porcine brain) 2 x 50 µg 5,605.60
Cytoskeleton RP01P-B Arp2/3 Protein Complex (Porcine brain) 6 x 50 µg 14,341.60
Cytoskeleton RR02-A Rap1b protein (Human wild type, His) 1 x 100 µg 3,640.00
Cytoskeleton RS01-A H-Ras-His Protein wild type 1 x 100 µg 3,640.00
Cytoskeleton RS01-C H-Ras-His Protein wild type 3 x 100 µg 9,100.00
Cytoskeleton RT01-A Rhotekin RBD protein GST fusion 1 x 500 µg 5,678.40
Cytoskeleton RT02-A Rhotekin RBD protein on GST beads 2 x 2 mg 7,207.20
Cytoskeleton RT02-B Rhotekin RBD protein on GST beads 6 x 2 mg 17,690.40
Cytoskeleton T238P-A Tubulin (porcine brain, >99% pure) 1 x 1 mg 2,256.80
Cytoskeleton T238P-B Tubulin (porcine brain, >99% pure) 5 x 1 mg 8,590.40
Cytoskeleton T238P-C Tubulin (porcine brain, >99% pure) 20 x 1 mg 25,407.20
Cytoskeleton T240-A Tubulin (porcine brain, >99% pure) 1 x 1 mg 1,601.60
Cytoskeleton T240-B Tubulin (porcine brain, >99% pure) 5 x 1 mg 7,644.00
Cytoskeleton T240-C Tubulin (porcine brain, >99% pure) 20 x 1 mg 25,261.60
Cytoskeleton T240-DX Tubulin (porcine brain, >99% pure) 1 x 10 mg 12,376.00
Cytoskeleton T333P-A Tubulin (Biotin Conjugate; Porcine Brain) 5 x 20 µg 4,295.20
Cytoskeleton T333P-B Tubulin (Biotin Conjugate; Porcine Brain) 20 x 20 µg 11,575.20
Cytoskeleton T333P-XL Tubulin (Biotin Conjugate; Porcine Brain) 1 x 500 µg 9,973.60
Cytoskeleton TA01-A Tau (bovine brain) 1 x 50 µg 4,222.40
Cytoskeleton TA01-B Tau (bovine brain) 3 x 50 µg 9,464.00
Cytoskeleton TL238-A Tubulin (bovine brain, >99% pure, lyophilized) 4 x 250 µg 2,620.80
Cytoskeleton TL238-B Tubulin (bovine brain, >99% pure, lyophilized) 1 x 1 mg 1,892.80
Cytoskeleton TL238-C Tubulin (bovine brain, >99% pure, lyophilized) 5 x 1 mg 7,862.40
Cytoskeleton TL440M-A Tubulin (AMCA dye labeled; porcine, micro-injctn) 5 x 20 µg 4,295.20
Cytoskeleton TL440M-B Tubulin (AMCA dye labeled; porcine, micro-injctn) 20 x 20 µg 11,575.20
Cytoskeleton TL488M-A Tubulin (HiLyte 488TM dye labeled; porcine, micro-injctn) 5 x 20 µg 4,295.20
Cytoskeleton TL488M-B Tubulin (HiLyte 488TM dye labeled; porcine, micro-injctn) 20 x 20 µg 11,575.20
Cytoskeleton TL590M-A Tubulin (Rhodamine labeled; porcine, micro-injctn) 5 x 20 µg 4,295.20
Cytoskeleton TL590M-B Tubulin (Rhodamine labeled; porcine, micro-injctn) 20 x 20 µg 11,575.20
Cytoskeleton TL620M-A Tubulin (X-rhodamine labeled; Bovine Brain micr-injctn) 5 x 20 µg 4,295.20
Cytoskeleton TL620M-B Tubulin (X-rhodamine labeled; Bovine Brain micr-injctn) 20 x 20 µg 11,575.20
Cytoskeleton TL670M-A Tubulin (HiLyte 647TM dye labeled; porcine, micro-injctn) 5 x 20 µg 4,295.20
Cytoskeleton TL670M-B Tubulin (HiLyte 647TM dye labeled; porcine, micro-injctn) 20 x 20 µg 11,575.20
Cytoskeleton TP005 Tubulin protein Soybean plant 1 x 250 µg 10,920.00
Cytoskeleton TXD01 Paclitaxel  (2mM in DMSO) 10 x 100 µl 2,475.20
Cytoskeleton V01-A Vimentin (Recombinant Syrian Hamster) 2 x 50 µg 2,402.40
Cytoskeleton V01-C Vimentin (Recombinant Syrian Hamster) 10 x 50 µg 11,211.20
Cytoskeleton VCG03-A VCA domain of WASP (GST fusion) 1 x 500 µg 6,188.00
Cytoskeleton WBM01 GOBlot Western Blot Processor each 10,920.00
Cytoskeleton WBM01 GOBlot Western Blot Processor four pack 37,856.00