| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VJI0300 | pAO815 | Invitrogen | 酵母表达载体 |
Comments for pAO815:
7709 nucleotides
5´ AOX1 promoter fragment: bases 1-940
5´ AOX1 primer site: bases 855-875
EcoR I Site: bases 943-948
3´ AOX1 primer site: bases 1024-1044
3´ AOX1 transcription termination （TT）:
bases 950-1277
HIS4 ORF: bases 4199-1665
3´ AOX1 fragment: bases 4554-5310
pBR322 origin: bases 6394-5740
Ampicillin resistance gene: bases 7399-6539
5´ AOX1 promoter region: bases 1-941
5´ AOX1 priming site: bases 855-875
α-factor signal sequence: bases 941-1207
Multiple cloning site: bases 1208-1276
c-myc epitope: bases 1275-1304
Polyhistidine （6xHis） tag: bases 1320-1337
3´ AOX1 priming site: bases 1423-1443
AOX1 transcription termination region: bases 1341-1682
TEF1 promoter: bases 1683-2093
EM7 promoter: bases 2095-2162
Sh ble ORF: bases 2163-2537
CYC1 transcription termination region: bases 2538-2855
pUC origin: bases 2866-3539 （complementary strand）