标签归档：invitrogen

invitrogen 代理产品

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invitrogen 代理产品

简要描述：Invitrogen是*的生命技术公司，公司创立于1987年，总部位于美国加利福尼亚州，在24个国家和地区设有分支机构，雇员超过4,800人，技术和产品广泛服务于70多个国家的学术和政府研究机构以及制药和生物技术公司，2005年收入达13亿美元。

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 Invitrogen是的生命技术公司，公司创立于1987年，总部位于美国加利福尼亚州，在24个国家和地区设有分支机构，雇员超过4,800人，技术和产品广泛服务于70多个国家的学术和政府研究机构以及制药和生物技术公司，2005年收入达13亿美元。Invitrogen主要为分子生物、疾病研究，药物研发和生物制品生产提供必要的生物技术产品和服务，研发力量主要集中在包括功能基因组、蛋白组学、生物信息学和细胞生物学等生命科学研究领域内的突破性创新，以便向进行生命科学研究的学术及政府研究机构以及医药和生物

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Invitrogen11668-027原装脂质体2000

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产品型号

品       牌

产品简介

本公司各种实验室生化试剂大量备现货，欢迎广大新老客户订购

详情介绍

脂质体2000 1.5ml Invitrogen11668-027原装 5
脂质体2000 原装 0.75ml Invitrogen11668-019原装 5
ProteinaseK蛋白酶K 100mg Merck原装 1
ProteinaseK蛋白酶K 100mg×10支 Merck原装 1
Hygromycin潮霉素B（原装） Roche原装843555 1g（20ml） 5
1640培养基 1640 Medium 10X1L原装 GIBCO货号：31800-022 10
DMEM（高糖） DMEM（High Glucose） 10X1L原装 GIBCO货号：12100046 10
DMEM（高糖）含丙酮酸钠 10X1L原装 GIBCO货号：12800017 10
DMEM（低糖）DMEM（Low Glucose） 10X1L原装 GIBCO货号：31600034 10
M199 Medium  M199培养基  10X1L原装 GIBCO货号：31100035 10
MEM培养基 10X1L原装 GIBCO货号：41500034 10
F12培养基  10X1L原装 GIBCO货号：21700075 10
D-MEM/F-12培养基 10X1L原装 GIBCO货号：12400-024 10
琼脂糖 100g 原装 西班牙 50
dNTPs（10mM/种） 1ml Roche分 50
Trizol Reaget原装GIBCO 100ml GIBCO15596-026 10
dNTPs （100mM） 4X0.1ml Roche分 四种分开 10
Thrombin  * 1000u SigmaT4648 10
Heparin Sodium  * 100g  国产 1
EB  溴化乙锭 1g SigmaE8751 10
Streptozotocin  链脲佐菌素 1g SigmaS0130 5
SephadexG-100  葡聚糖凝胶G-100 100g pharmacia17-0061-02 1
SephadexG-75  葡聚糖凝胶G-75  100g pharmacia17-0050-01 1
SephadexG-25  葡聚糖凝胶G-25  100g pharmacia17-0033-01 1
SephadexLH-20  葡聚糖凝胶LH-20  100g pharmacia 17-0710-01 1
DEAE Sephadex  葡聚糖凝胶A-25 100g pharmacia17-0170-01 1
DEAE Sephadex  葡聚糖凝胶A-50 100g pharmacia17-0180-01 1

*INVITROGEN血清替代品，货号10828-028！

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我公司帮助客户*若干瓶血清替代产品，用于干细胞培养用，INVITROGEN公司，货号10828-028，500ml的，*，有需要，请随时。
 

Description

Knockout™ Serum Replacement （Knockout™ SR） is a defined, serum-free formulation optimized to grow and maintain undifferentiated ES cells in culture （1）. It directly replaces FBS in existing protocols. The performance of Knockout™ SR is enhanced when used with Knockout™ D-MEM. Product performance is confirmed by evaluation of its ability to support the growth of undifferentiated D3 ES cell colonies on inactivated mouse embryonic fibroblasts.

We also offer the KnockOut™ ESC/iPSC Media Kit, an entire media system for feeder-based culture of pluripotent stem cells in one product.

 

CALTAG公司介绍

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CALTAG公司介绍

简要描述：美国CALTAG Laboratories公司创建于1985年，是美国主要的流式试剂生产商，2005年成为Invitrogen公司旗下的一员。

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公司：http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-and-Tissue-Analysis/Antibodies-and-Secondary-Detection.html

美国CALTAG Laboratories公司创建于1985年，是美国主要的流式试剂生产商，2005年成为Invitrogen公司旗下的一员

Invitrogen GeneSwith 哺乳动物诱导系统pSwitch 说明书

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 Invitrogen GeneSwith 哺乳动物诱导系统

Invitrogen

Vectors：pSwitch

产品参数: 

Mammalian Selection：Hygromycin

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

Switch-On Expression from the Lowest Basal Levels

The GeneSwitch? System for inducible mammalian expression is ideal for experiments that require the absolute lowest uninduced expression levels. The expression vector pGene/V5-His provides a minimal promoter, GAL4-E1b, consisting of the binding sites for the yeast Gal4 DNA binding protein followed by the TATA sequence from the Adenovirus E1b promoter. Without additional factors, the GAL4-E1b promoter is transcriptionally silent.

To activate transcription from the GAL4-E1b promoter, the GeneSwitch? regulatory protein is expressed from a minimal TK promoter on the pSwitch vector. The GeneSwitch? protein has three functional domains:

  1. Gal4 DNA Binding Domain （Gal4-DBD） to bind the regulatory protein to the GAL4-E1b promoter
  2. Truncated human Progesterone Receptor Ligand Binding Domain （hPR-LBD） that undergoes a conformational change when it binds the progesterone antagonist, mifepristone
  3. Transcription activation domain from the NF?B transcription factor p65 （p6D） to activate transcription from the silent GAL4-E1b promoter

In the absence of mifepristone, the conformation of the hPR-LBD region prevents the GeneSwitch? regulatory protein from activating transcription from the GAL4-E1b promoter. When mifepristone is added and binds the hPR-LBD region, the GeneSwitch? regulatory protein assumes a conformation that permits it to stimulate transcription from the GAL4-E1b promoter.

Induction of the GeneSwitch? System leads to activation of the gene of interest on the vector pGene/V5-His. In addition, four Gal4 binding sites upstream of the minimal HSV TK promoter on pSwitch can bind the pSwitch regulatory protein. Therefore, adding mifepristone up-regulates production of the regulatory protein. The increased levels of the GeneSwitch? regulatory protein result in induction of the gene of interest from pGene/V5-His to levels that can approach those of viral promoters.

The GeneSwitch? Kit offers exceptionally low uninduced and high induced expression levels in mammalian cells. The system provides the expression vector pGene/V5-His with the minimal, synthetic GAL4-E1b promoter that is transcriptionally silent until activated. The GeneSwitch? regulatory protein binds the GAL4-E1b promoter to activate transcription upon the addition of mifepristone. In addition, the GeneSwitch? protein upregulates its own expression, leading to amplified expression of the gene of interest from pGene/V5-His. pGene/V5-His offers several features that facilitate expression analysis and purification of recombinant proteins in mammalian cells:

  1. C-terminal V5 epitope tag for detection with Anti-V5 Antibodies
  2. C-terminal polyhistidine （6xHis） tag for rapid purification with nickel-chelating resin and detection with Anti-His（C-term） Antibodies
  3. The Zeocin? resistance gene for effective selection in both mammalian cells and E. coli