分类目录归档：日本 Jaica老化

日本 Jaica老化制御研究所代理

发表于由

日本 Jaica老化制御研究所代理

日本老化制御研究所自成立以来就对抗衰老进行研究，它是在有助于健康促进的理念下创建的。结果，此研究所在开发DNA氧化损伤测试试剂盒方面取得了很大的成功，并在该领域处于了领先地位。
本研究所现在销售许多氧化应激相关产品，这些产品可应用于医学，药学，生化学，食品科学等。它还利用其独特的技术开展检测服务。随着社会老龄化的发展，预防医学与抗衰老引起了越来越多的关注，在这些领域中，我们研究所将会通过开发和提供研究产品来助于营造一个健康，长寿的社会。
 
产品类型：
1. DNA氧化检测产品；
2. 蛋白氧化检测产品；
3. 脂肪氧化检测产品；
4. 抗氧化检测产品。
 
特性：
1. 高专一性、高灵敏性；
2. 不与RNA损伤产生交叉反应；
3. 易于操作、简单快速；
4. 无需昂贵的设备和样品预处理。
日本Jaica
The Japan Institute for the Control of Aging has conducted research into control of aging since its foundation under the doctrine of contributing health promotion. As a result, the institute succeeded in developing a DNA oxidative damage measuring kit, thus taking the lead for this field.
The institute now sells many products related to oxidative stress, and such products are utilized in the fields of medicine, pharmacy, biochemistry, food sciences, etc. The institute also performs assay services utilizing its unique technologies. Preventive medicine and anti-aging are receiving increased attention with the advancement of aging society. In these areas, our Institute will contribute to realizing a healthy, long-lived society by developing and supplying research products.
Type of
marker		 Item Spec. Applications Remark
Urine Serum Cells
DNA
oxidation
marker		 8-OHdG
New 8-OHdG Check ELISA
(Code. KOG-200SE), ELISA: 450nm, 96 wellsHighly Sensitive 8-OHdG Check ELISA
(Code. KOG-HS10E), ELISA: 450nm, 96 wells
Anti 8-OHdG monoclonal antibody (N45.1)
(Code: MOG-020P / MOG-100P)		 Assay range:
0.5 – 200 ng/mLAssay range:
0.125-10 ng/mL
20 µg /
100 µg of IgG		 OKOK
–		 OKOK
–		 -OK
OK		 Suitable for urine and animal serum. Assay time: 2.5 hours.(KOG-200SE)Suitable for human serum,tissue and cultured cells. Assay time: overnight +1.5 hours(KOG-HS10E)
Highly specific to 8-OHdG. Low cross reactivity to oxidized RNA(8-OHGua/8-OHG).
Anti Thymidine Glycol (TG) antibody
(Code: MTG-100P) Clone 2E8		 100 µg of IgG – – OK DNA-specific oxidative stress marker. Specific to TG in DNA polymer.
Lipid
oxidation
marker		 HEL (13HPODE-Lys adduct)
Hexanoyl-Lysine (HEL) ELISA kit
(Code: KHL-700E), ELISA: 450nm, 96 wellsAnti Hexanoyl-Lysine (HEL) antibody
(Code: MHL-021P) Clone 5F12		 Assay range:
2 – 700 nmol/L20 µg of IgG		 OK- OK- OKOK Nε-Hexanoyl-Lysine (HEL) is derived from 13-HPODE, and is a novel biomarker for early stage oxidation of n:6 polyunsaturated fatty acid. Detectable in urine, serum, tissue and cultured cells.
4-HNE
Anti 4-HNE monoclonal antibody (HNEJ2)
(Code: MHN-020P / MHN-100P)		 20 µg /
100 µg of IgG		 – – OK 4-hydroxy-2-nonenal (4-HNE) is one of the major oxidized products of fatty acids. Specific to 4-HNE-modified proteins.
MDA
Anti MDA monoclonal antibody
(Code: MMD-030n) Clone 1F83Malondialdehyde assay kit (TBARS)
(Code: KMD-008W) Colorimetric: 400-700nm scan		 30 µg of IgGAssay range:
0.08 – 10 µmol/L		 -OK -OK OKOK Specific to Malondialdehyde(MDA)-protein adducts such as dihydropyridine-lysine (DHP-lysine).
By utilizing 3rd derivative analysis, more reliable results than that by traditional TBARS assay.
Isoprostane
Urinary isoprostane ELISA
(Code: KIP-050) ELISA: 450nm, 96wellsSerum isoprostane ELISA
(Code: KIP-001W) ELISA: 450nm, 96wells		 Assay range:
0.05 – 100 ng/mLAssay range:
0.05 – 10 ng/mL		 OK- -OK -OK 15-Isoprostane F2t (also known as 8-epi-PGF2α or 8-iso-PGF2α) is produced by peroxidation of lipoproteins.
For serum or tissue samples, purification by C18/Silica Sep-Pak will be needed.
Anti Acrolein (ACR) antibody
(Code: MAR-020n / MAR-100n) Clone 5F6		 20 µg /
100 µg of IgG		 – – OK Specific to ACR modified proteins: formyl-dehydropiperidino-lysine (FDP-lysine).
Anti 4-hydroxy-2-hexenal (4-HHE) antibody
(Code: MHH-030n) Clone HHE53		 30 µg of IgG – – OK Specific to HHE-histidine Michael adduct (HHE-His).
Anti Crotonaldehyde (CRA) antibody
(Code: MCA-030n) Clone 82D3		 30 µg of IgG – – OK Specific to CRA-protein aduucts.
Anti Methylglyoxal (MG) antibody
(Code: MMG-030n) Clone 3C		 30 µg of IgG – – OK Specific to MG-protein adduct (argpyrimidine).
Anti 7-ketocholesterol (7-KC) antibody
(Code: MKC-020n) Clone 35A		 20 µg of IgG – – OK Biomarker of cholesterol oxidation. Applicable for frozen tissue only.
Mitochondria
Lipid peroxide probe (mitochondria) [NEW]
(Code: PLP-466J)		 5 µg x3 – – OK Fluorescence probe for lipid peroxides specific to mitochondia. Suitable for cultured cells.
Lipid peroxide probe (DPPP) [NEW]
(Code: PLP-350J)		 10 mg – OK OK Fluorescence probe for lipid peroxides. For HPLC application.
Protein
oxidation
marker		 Dityrosine (Tyr dimer)
Dityrosine (DT) ELISA [NEW]
(Code: KDT-010E), ELISA: 450nm, 96 wellsAnti Dityrosine (DT) antibody
(Code: MDT-020P) Clone 1C3		 Assay range:
0.05-12 µmol/L20 µg of IgG		 OK- OK- -OK New biomarker specific for protein oxidation. Assay time: overnight + 1.5hours.
Anti Dibromo-Tyrosine (DiBrY) antibody
(Code: MBY-020P) Clone 3A5		 20 µg of IgG – – OK Inflammation-related protein oxidation marker.
NO-modification
Anti Nitrotryptophan (NW) antibody [NEW]
(Code: MNW-020P) Clone 117C		 20 µg of IgG – – OK A novel nitrosative stress marker derived from peroxynitrite and tryptophan.
Nitrotyrosine ELISA
(Code: KNT-002W) 96 wells		 2 – 1500 nmol/L – OK Δ
Anti-
oxidant
assay		 Total Antioxidant
Test kit for Potential Antioxidant(PAO), 96 wells
(Code: KPA-050) Colorimetric: 480-492 nmTAC Peroxyl assay,  96 wells
(Code: KPR-005W) Luminescence		 Assay range:
21.9- 4378 µmol/LAssay range:
0.5～30 µmol/mL		 — OKOK OKOK Total antioxidant assay for serum, foods and beverages. Can detect both hydrophobic and hydrophilic antioxidants.
KPR-005W can detect peroxyl radicals(ROO·) scavenging activity.
GSH
Total Glutathione assay kit 100 tests [NEW]
(Code: KGS-419J) Colorimetric: 405-415 nm		 Assay range:
5-100 µmol/L		 – – OK The most abundant antioxidant in living cells. Applicable to a variety of samples such as plant tissues and bacteria.
Enzyme
assay		 SOD
SOD activity assay kit (WST-1 method)  [NEW]
(Code: KSD-311J) Colorimetric: 450 nm, 500 testsSOD activity assay kit (Hematoxylin method)
(Code: KSD-005W) Colorimetric: 560 nm, 96 wells
Cu/Zn-SOD ELISA (Human SOD1)
(Code: KSE-040W) ELISA: 450 nm, 96 wells
Mn-SOD ELISA (Human SOD2)
(Code: KSG-025W) ELISA: 450 nm, 96 wells
ec-SOD ELISA (Human SOD3)
(Code: KSF-002W) ELISA: 450 nm, 96 wells		 Assay range:
5-60 U/mL
Assay range:
0.04 – 5 ng/mL
Assay range:
25 – ng/mL
Assay range:
2 – 168 ng/mL		 —
–
–
–		 OKEryth-
rocyte
OK
–
–		 OKOK
OK
OK
OK		 Can be applied to all animals, plants and bacteria.SOD1 is mainly located in erythrocytes and tissue cells. Trace concentration of SOD1 would be detected in serum.
Mn-SOD(SOD2) is detectable in Mitochondria.
CAT
Catalase activity assay kit 96 wells
(Code: KCA-003W) UV: 240 nm		 Assay range:
6-150 U/mL		 – Eryth-
rocyte		 OK
MPO activity assay kit
(Code: KMP-008W) Colorimetric: 412 nm
96 microplate assays or 30 cubette assays		 Assay range:
8- 400 U/mL		 – OK OK
GSH metabolism
Glutathion Peroxidase (GPx) assay kit 192 wells
(Code: KGX-005W) Colorimetric: 340 nmGlutathion Reductase (GR) assay kit 192 wells
(Code: KGR-015W) Colorimetric: 340 nm		 Assay range:
5-60 mU/mLAssay range:
1.5-20 mU/mL		 — BloodBlood OKOK
Redox
regulation
markers		 Thioredoxin
Thioredoxin I ELISA (human) 96 wells
(Code: KRX-004W) ELISA: 450 nm		 Assay range:
0.4- 25 ng/mL		 – OK Δ
Thioredoxin reductase I ELISA (human) 96 wells
(Code: KRR-004W) ELISA: 450 nm		 Assay range:
0.4- 25 ng/mL		 – OK Δ
Peroxiredoxin I ELISA (human) 96 wells
(Code: KPX-005W) ELISA: 450 nm		 Assay range:
0.5 – 32 ng/mL		 – OK Δ
Peroxiredoxin III ELISA (human) 96 wells
(Code: KPZ-002W) ELISA: 450 nm		 Assay range:
2 – 32 ng/mL		 – OK Δ
Trace
elements
assay		 Fe
Iron assay kit (Ferrozine) 100 tests
(Code: CFE-005) Colorimetric: 540-580 nmIron assay kit (Nitroso-PSAP) 100 tests
(Code: CFE-010) Colorimetric: 740-760 nm
UIBC assay kit (Bathophenanthroline) 100 tests
(Code: CBC-800) Colorimetric: 540-550 nm		 Assay range:
5-1,000 µg/dLAssay range:
10-1,000 µg/dL
Assay range:
10-800 µg/dL		 OKOK
–		 OKOK
OK		 OKOK
–		 Iron (Fe) is an important pro-oxidant factor which produce hydroxyl radical by fenton reaction. CFE-005 and CFE-010 can be also applied to extractes from animal tissue, plants and hair samples.Detection of unsaturated iron binding capacity(UIBC). Species independent.
Cu
Copper assay kit (3,5-DiBr-PAESA) 100 tests
(Code: CCU-400) Colorimetric: 570-590 nm		 Assay range:
3-400 µg/dL		 OK OK OK Can detect total (free and ceruloplasmin – bond) copper. Also applicable to extractes from tissues, plants and hair.
Zn
Zink assay kit (5-Br-PAPS)
(Code: CZN-001) Colorimetric: 550-580 nm		 Assay range:
4-1,000 µg/dL		 OK OK OK Can detect total (free and protein – bond) zinc. Also applicable to extractes from tissues, plants and hair.
Ca
Calcium assay kit (Chlorophosphonazo III)
(Code: CCA-020) Colorimetric: 680-700 nm, 200 testsCalcium assay kit (o-Cresolphtalein-Complexone)
(Code: CCA-030) Colorimetric: 560-590 nm, 200 tests		 Assay range:
0.05-20 mg/dLAssay range:
0.2-30 mg/dL		 OKOK OKOK OKOK Applicable to serum, plasma, urine, saliva, extracts from tissues, plants, hair and bone samples. Avoid EDTA containing samples.
Mg
Magnesium assay kit (Xyridil Blue-I) 100 tests
(Code: CMG-035) Colorimetric: 660 nm		 Assay range:
0.05-3.5 mg/dL		 OK OK OK Applicable to serum, plasma, urine, saliva, extracts from tissues, plants and hair. May be interfared by EDTA, haemolysis, anticoagulants and potassium.

日本 JaICA代理 Anti Di Tyrosine (DT) 单抗

发表于由

日本 JaICA代理 Anti Di Tyrosine (DT) 单抗
日本老化制御研究所自成立以来就对抗衰老进行研究，它是在有助于健康促进的理念下创建的。结果，此研究所在开发DNA氧化损伤测试试剂盒方面取得了很大的成功，并在该领域处于了领先地位。
本研究所现在销售许多氧化应激相关产品，这些产品可应用于医学，药学，生化学，食品科学等。它还利用其独特的技术开展检测服务。随着社会老龄化的发展，预防医学与抗衰老引起了越来越多的关注，在这些领域中，我们研究所将会通过开发和提供研究产品来助于营造一个健康，长寿的社会。
产品类型：
DNA氧化检测产品；
蛋白氧化检测产品；
脂肪氧化检测产品；
抗氧化检测产品。
特性：
高专一性、高灵敏性；
不与RNA损伤产生交叉反应；
易于操作、简单快速；
无需昂贵的设备和样品预处理。
上海金畔生物作为日本 JaICA 老化中国代理商，欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
日本 JaICA代理 Anti Di Tyrosine (DT) 单抗
产品名称： Anti Di Tyrosine (DT) 单抗
英文名称： Anti Di Tyrosine (DT) monoclonal antibody
产品货号： MDT-020P
产品规格： 20ug
产品产地： 日本
产品商标： JaICA
 

Code Product Name  
KOG-200SE New 8-OHdG Check ELISA kit 96 wells
KOG-200TE New 8-OHdG Check ELISA Trial Package (32wells) 32 wells
KOG-HS10E Highly Sensitive 8-OHdG Check ELISA kit 96 wells
MOG-100P Anti 8-OHdG monoclonal antibody 100 micro gram
MOG-020P Anti 8-OHdG monoclonal antibody 20 micro gram
MHN-100P Anti 4-HNE monoclonal antibody 100 micro gram
MHN-020P Anti 4-HNE monoclonal antibody 20 micro gram
MBY-020P Anti Di bromo tyrosine (DiBrY) monoclonal antibody 20 micro gram
MTG-100P Anti Tymidine Glycol (TG) monoclonal antibody 100 micro gram
MDT-020P Anti Di Tyrosine (DT) monoclonal antibody 20 micro gram
MHL-021P Anti Hexanoyl-Lys(HEL) monoclonal antibody (clone 5F12) 20 micro gram
KHL-700E Hexanoyl-Lys(HEL) ELISA kit 96 wells
KPA-050 Test kit for Potential Anti Oxidant (PAO) 96 wells
MMD-030n Anti malondialdehyde (MDA) monoclonal antibody 30 micro gram
MAR-020n Anti acrolein (ACR) monoclonal antibody 20 micro gram
MAR-100n Anti acrolein (ACR) monoclonal antibody 100 micro gram
MHH-030n Anti 4-hydroxy-2-hexenal (4-HHE) monoclonal antibody 30 micro gram
MCA-030n Anti crotonaldehyde (CRA) monoclonal antibody 30 micro gram
MMG-030n Anti methyglyoxal (MG) monoclonal antibody 30 micro gram
MKC-020n Anti 7-ketocholesterol (7-KC) monoclonal antibody 20 micro gram
OPF-010E NEW Ultra filter unit for serum pretreatment (MW cut off 10kDa, Pall Corp.) 1 unit
KDT-010E Dityrosine (DT) ELISA  96 wells
OPNUCP1 Nuclease P1 from Penicillium citrinum 500 units

 

日本 JaICA代理 Anti HEL monoclonal antibody

发表于由

日本 JaICA代理 Anti HEL monoclonal antibody
日本 JaICA 老化 Antibody系列产品
上海金畔生物作为日本 JaICA 老化中国代理商，欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。
日本老化制御研究所 Jaica代理
抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
适用于免疫组织化学和ELISA。
Anti hexanoyl-Lysine adduct monoclonal antibody.
Suitable for immunohistocheistry and ELISA. For research use only.
Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.
由活性氧（ROS）引起的脂质氧化损伤在一些疾病，细胞功能损害和衰老中起重要作用。醛类（如丙二醛/MDA）和4-羟基-2-壬烯醛（4-HNE）已被报道为先进的脂质过氧化产物之一，但最近在脂质过氧化的早期阶段，发现13-hydroperoxyoctadecanoic acid(13-HPODE)与蛋白质1)共价结合。
己酰-赖氨酸加合物（HEL）是一种新的脂质氢过氧化物修饰的赖氨酸残基， HEL通过氧化的omega-6脂肪酸（例如亚油酸或花生四烯酸）氧化修饰形成。
抗己酰-赖氨酸（HEL）单克隆抗体对于含有HEL的蛋白质和肽是特异的，适用于通过免疫组织化学和免疫印迹的HEL检测。
A new biomarker for lipid peroxidation   脂质过氧化新的生物标志物


Cisplatin-treated rat kidney. 顺铂处理的大鼠肾脏
(Dr. Sugiyama, Tottori Univ.)  （Sugiyama博士）

Detection of HEL at Human atherosclerotic lesions.
(Dr. Naito)
HEL在人类动脉粥样硬化病变的检测  （Naito博士）

Clone #: 5F12
Subclass: IgG1 kappa
Antigen: N epsilon hexanoyl-Keyhole limpet hemocyanin (HEL-KLH)
Specificity: Specific to Hexanoyl-Lys adducts.
Form: Lyophilized power. Containing 10mM PBS(pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950. 100 碌g/mL of IgG.
Application: Immunohistochemistry
(Recommended antibody concentration is 2 碌g/mL on paraformaldehyde fixed tissue), and ELISA
Storage: Less than -20C

• 技术参数
克隆＃：5F12；
亚基：IgG1 kappa；
抗原：N epsilon己酰-钥孔血蓝蛋白（HEL-KLH）；
特异性：特异于己酰-赖氨酸加合物；
形式：冻干粉，100μg/mL（200μL），PBS（pH7.4），包含5％蔗糖，1％ BSA；
应用：免疫组织化学（多聚甲醛固定的组织推荐抗体浓度为2μg/mL）和ELISA；
储存：低于-20°C
参考文献：

1) Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)
Identification of HEL, which is lysine adduct of 13-HPODE.
2) Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)
Development and characterization of anti HEL monoclonal antibody.
3) Fukuchi Y, Miura Y, Nabeno Y, Kato Y, Osawa T, Naito M, Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions. J Atheroscler Thromb 15(4)p185-192(2008)
Application to rabbit atherosclerotic lesions.
4) Maeda R, Noiri E, Isobe H, Homma T, Tanaka T, Negishi K, Doi K, Fujita T, Nakamura E, A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells. Hypertens Res 31(1)p141-151(2008)
Application to cultured cells (HUVECs).
5) Sango K, Yanagisawa H, Kato K, Kato N, Hirooka H, Watabe K: Differential Effects of High Glucose and Methylglyoxal on Viability and Polyol Metabolism in Immortalized Adult Mouse Schwann Cells. Open Diabetes J. 1, p1-11(2008)
Application to cultured cell from mouse.

产品信息：

Product name Code Specifications Application NOTICE
Anti HEL monoclonal antibody(clone 5F12) MHL-021P 20ug of IgG Immunohistochemistry Current product
Anti HEL monoclonal antibody(clone 5H4) MHL-020P 20ug of IgG Immunohistochemistry Discontinued

使用说明书
免疫组织化学方法
技术信息
克隆#5F12和5H4的比较

Instruction manual (PDF) Immunohistochemical procedure Technical information
Comparison of clone 5F12 and 5H4 (PDF)