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Hypoxyprobe™ Plus Kit
(HPI Part # HP2-XXX)
Kit contents:
Solid pimonidazole HCl (Hypoxyprobe™-1)
FITC conjugated to anti-pimonidazole mouse IgG1 monoclonal antibody (FITC-MAb1) and
Rabbit anti-FITC conjugated with horseradish peroxidase as a secondary reagent.
Applications: Immunochemical detection of cell and tissue hypoxia through immunofluorescence or immunoperoxidase staining, western blotting, or flow cytometry. There is very low background in mouse tissues when the Hypoxyprobe™ Plus Kit is combined with an anti-FITC secondary reagent.
Quantities: a. Hypoxyprobe™ Plus Kits contain100 mg, 200 mg or 1000 mg of pimonidazole HCl. Typical doses are 60mg/kg body weight in small animal studies and 14mg/kg body weight for human studies. b. One vial (100 and 200 mg Kits) or two vials (1000 mg Kit) containing 200 uL of an anti-pimonidazole, FITC-conjugated IgG1 mouse monoclonal antibody (clone 4.3.11.3). Each vial contains 200 microliters of a 0.50 mg/ml solution of the FITC-MAb1 in PBS containing 1 % BSA and 0.09% sodium azide. Optimal dilution of the FITC-MAb1 is to be determined by the investigator but 1:50 or 1:100 dilutions have been found to give strong immunostaining in mouse tumor tissue when combined with a 1:50 or 1:100 dilution of the peroxidase conjugated anti-FITC secondary reagent. While not designed specifically for immunofluorescence, FITC-MAb1 can be used for this purpose. Antibody dilution is typically lower for immunofluorescence than for immunoperoxidase detection.
Not supplied: Standard reagents used for immunohistochemical analyses.
Storage: a. Store Hypoxyprobe™-1 solid at room temperature in the dark (can also be stored at 2- 8 degrees C). b. Store FITC-MAb1 and HRP conjugated rabbit anti-FITC at 2-8 degrees C in the dark. Do not freeze.