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Anti-NMDA Receptor 2A (GluN2A) (extracellular)
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| Ionotropic Glutamate receptor subunit ε1, N-methyl-D-aspartate receptor subunit 2A, GRIN2A, GLUN2A |
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- Cat #: AGC-002
- Size: 25 µl, 50 µl, 0.2 ml
- Source: Rabbit
- Type: Polyclonal
一般信息
| Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GluN2A. Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody (#AGC-002) can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry. The antibody recognizes an extracellular epitope and is thus ideal for detecting GluN2A in living cells. It has been designed to recognize NR2A from rat, mouse, and human samples.
Related products for control experiments:
Control peptide antigen (supplied with the antibody free of charge).
Anti-NMDA Receptor 2A (GluN2A) antibody (#AGC-002) in the literature:
Western blot analysis (WB):
– Mouse brain lysate (1:2000) (see Konstantoudaki, X. et al. (2016) in Product Citations).
– Mouse hippocampus lysate (1:1000) (see Tindi, J.O. et al. (2015) in Product Citations).
– Mouse brain lysate (1:500) (see Atkin, G. et al. (2015) in Product Citations).
Immunohistochemistry (IH):
– Mouse brain sections (1:100) (see Atkin, G. et al. (2015) in Product Citations).
Immunocytochemistry (IC):
– Human induced pluripotent stem cells (1:300) (see ezhkin, V. et al. (2016) in Product Citations).
– Mouse hippocampal neurons (see Atkin, G. et al. (2015) in Product Citations).
– Rat cultured hippocampal neurons (1:100) (see Swanger, S.A. et al. (2013) in Product Citations).
Live cell imaging (LCI):
– Rat hippocampal neurons (see Dupuis, J.P. et al. (2014) in Product Citations).
– Rat hippocampal primary neurons (1:500) (see Mikasova, L. et al. (2012) in Product Citations). |
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| Western blot |
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| Western blot analysis of rat brain lysates: |
1. Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody (#AGC-002), (1:600).
2. Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody, preincubated with the control peptide antigen. |
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| Live cell imaging / Immunocytochemistry |
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| Rat live C6 glioma cells (1:100). See immunocytochemistry. |
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| Immunoprecipitation |
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| Immunoprecipitation of rat brain lysates |
1. Cell lysate.
2. Cell lysates + protein A beads + Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody (#AGC-002).
3. Cell lysates + protein A beads + pre-immune rabbit serum.
Black arrow indicates the NMDA Receptor 2A protein while the red arrow shows the IgG heavy chain. Immunoblot was performed with Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody. |
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| Immunohistochemistry |
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| Immuno-colocalization of GluN2A and GluN2B in mouse deep cerebellar nucleus |
| Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDA Receptor 2B (GluN2B) (extracellular)-ATTO-594 antibody (#AGC-003-AR), (1:60) and Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody (#AGC-002), (1:200). A. Sections were incubated with Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody, followed by goat anti-rabbit-Alexa-488 (green). B. The same sections were incubated with Anti-NMDA Receptor 2B (GluN2B) (extracellular)-ATTO-594 antibody (red). C. Merge of A and B demonstrates the ubiquitous colocalization of the GluN2A and GluN2B subunits in cells with neuronal profiles in this nucleus. Arrows point at an example of NR2A and NR2B co-expression. |
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| Expression of NMDA Receptor 2A in rat hippocampus |
| Immunohistochemical staining of rat hippocampal dentate gyrus with Anti-NMDA Receptor 2A (GluN2A) (extracellular) antibody (#AGC-002). A. NMDA Receptor 2A (green) appears diffusely in the outer molecular layer of the dentate gyrus (Out Mol.) and in cells along the subgranular layer (arrows). B. Staining of parvalbumin (PV, red) identifies interneurons in the dentate gyrus. C. Confocal merge demonstrates localization of PV in some neurons with NMDA Receptor 2A. |
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| Immunocytochemistry |
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| Expression of NMDA Receptor 2A in rat C6 glioma cells |
Immunocytochemical staining of live intact rat C6 glioma cells.
A. Cells were stained with Anti-NMDA Receptor 2A (GluN2A) (extracellular)antibody (#AGC-002), (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). Cell nuclei were stained with the cell permeable dye Hoechst 33342 (blue staining). B. Live view of the same field. |
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