Anti-TTF1 – Rabbit (WRAB-1231)使用说明

Anti-TTF1 – Rabbit (WRAB-1231)使用说明

产品信息

Seven Hills Bioreagents

WRAB-1231

100 µL

宿

反应种属

人、鼠

克隆类型

多克隆

0.01% sodium azide

重组鼠TTF-1融合6*his蛋白

保存条件

储存在-20,避免反复冻融

蛋白背景

甲状腺转录因子-1TTF-1)是同源域转录因子家族的成员,并且在调节甲状腺,肺和脑内表达的基因中起作用。

产品使用

实验应用

建议稀释倍数

Western Blot

1: 5000

Immunohistochemistry

1:1000 – 1:2000

说明:最佳稀释倍数与浓度应由研究人员确认

应用实例

图片成年小鼠肺组织,用6%正常山羊血清预包埋,并用抗大鼠TTF-1兔血清(1:2000稀释)染色。通过DAB(黑色)观察TTF-1,并用核快红(粉红色)复染。放大倍数10倍。

参考文献

1. Zhou L, Lim L, Costa RH, Whitsett JA. 'Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung.' J Histochem Cytochem. 1996 Oct;44(10):1183-93.

2. Stahlman MT, Gray ME, Whitsett JA. 'Expression of thyroid transcription factor-1(TTF-1) in fetal and neonatal human lung.' J Histochem Cytochem. 1996 Jul;44(7):673-8.

3. Khoor A, Whitsett JA, Stahlman MT, Olson SJ, Cagle PT. 'Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma.' Hum Pathol. 1999 Jun;30(6):695-700.

4. deFelice M, Silberschmidt D, DiLauro R, Xu Y, Wert SE, Weaver TE, Bachurski CJ, Clark JC, Whitsett JA. 'TTF-1 phosphorylation is required for peripheral lung morphogenesis, perinatal survival, and tissue-specific gene expression.' J Biol Chem. 2003 Sep 12;278(37):35574-83.

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