Pentapharm 蛇毒蛋白质

 

 

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英文版:Snake Venom Proteins

Activator

Source

Application

Remarks

 

 

113-01 / 113-04

Protac®

• Determination of protein C and protein S
• Protein C pathway assays

Serine protease Agkistrodon contortrix

116-01

Ecarin

• Prothrombin assay
• Determination of hirudin

Metalloprotease, activates specifically prothrombin via meizothrombin, does not need PL and Ca2+ Echis carinatus

119-02

Convulxin

Activation of human plaets via binding and clustering of GPVI-receptors under physiological conditions

Crotalus durissus

121-03

RVV Factor V-Activator

Activation of FV in APC-R assay

Serine protease, not inhibited by AT

121-06

RVV-X

• Determination of FX
• Screening assay for lupus anti-coagulants (diluted Russel viper venom test, dRVVT)

Metalloprotease, does also (weakly) activate protein C and factor IX Daboia (Vipera) russelli

800191

Pefakit® Reptilase® Time

Ready-to-use diagnostic reagent for measurement of
• functionality of fibrin poly-merisation
• afibrinogenemia or hypofibrinogen-emia
• hyperfibrinolysis
• thrombolytic therapy

CE-marked IVD Bothrops atrox

 

中文版:

蛇毒蛋白质

活化剂

资源

应用

备注

113-01 / 113-04

Protac®

•蛋白C和蛋白S的
测定•蛋白C途径测定

丝氨酸蛋白酶Agkistrodon contortrix

116-01

蝰蛇毒

•凝血酶原测定
•水蛭素测定

金属蛋白酶,通过凝血酶原特异性激活凝血酶原,不需要PL和Ca2 + Echis carinatus

119-02

惊厥

在生理条件下通过结合和聚集GPVI-受体激活人血小板

响尾蛇durissus

121-03

RVV因子V激动剂

在APC-R测定中激活FV

丝氨酸蛋白酶,不受AT抑制

121-06

RVV-X

•FX的测定
•狼疮抗凝剂的筛选测定(稀释的罗素毒蛇毒测试,dRVVT)

金属蛋白酶,也(弱)激活蛋白C和因子IX Daboia(Vipera)russelli

800191

Pefakit®Reptilase®时间

即用型诊断试剂,用于测量
纤维蛋白聚合物的功能
•纤维蛋白原血症或纤维蛋白原 – 肌原纤维•纤维蛋白
溶解
•溶栓治疗

CE标记的IVD Bothrops atrox

 附件为113-01说明书。

Description:        Protac, a single chain glycoprotein, is a fast-acting protein C activator isolated from the venom of the copperhead snake Agkistrodon contortrix and closely related snake species. This serine proteinase rapidly converts protein C of man and other vertebrates into activated protein C which may be determined either by measuring its prolonging effect on the activated partial thromboplastin time (APTT) or by measuring its enzyme activity by means of a specific

 

chromogenic substrate.

 

 

Application:

 

Determination of protein C and protein S levels.

 

Origin:

 

Agkistrodon contortrix snake venom

 

MW: 36’000 to 42’000

 

Assay:

 

Chromogenic method

 

Clotting method

 

0.050 ml human citrated plasma

0.025 ml human citrated plasma

 

0.050 ml Protac  (0.5 unit/ml)

0.025 ml Protac  (0.1 unit/ml)

 

=> incubate for 5 min at 37°C

0.025 ml Actin 

 

0.800 ml 50 mM Tris-HCl buffer, pH 8.4

=> incubate for 4 min at 37°C

 

0.100 ml PefachromePCa (4 mM)

=> Determination of DOD/min at 405 nm

0.025 ml 25 mM CaCl2

=> Determination of clotting time

 

 

Unit definition:   1 Unit is the amount of Protac   which activates the amount of protein C contained in 1 ml of normal citrated human plasma within 5 min at 37°C.

 

 

Stability:

Lyophilised vials:

Reconstituted vials:

5 years

30 days at 2 – 8°C, approx. 7 days at 37°C

 

 

1 year at -20°C, 1 month at 20°C.

 

 

Storage: May be used by the expiry date given on the label when stored unopened, protected from moisture, in the dark, 2 – 8°C. Avoid contamination of the reagents by micro-organisms. Shipment of product does not require cooling during the time of transportation.

 

References: Bastian S. Protein C-Diagnostik – Methoden, Interpretation und Limitationen. ellipse 2002; 18 (1): 11-16

 

Gempeler-Messina PM, Volz K, Bühler B, Müller C. Protein C Activators from Snake Venoms and Their Diagnostic Use. Haemostasis 2001; 31:266-72.

 

Robert A, Eschwege V, Hameg H, Drouet L, Aillaud MF. Anticoagulant response to Agkistrodon contortrix venom (ACV test): a new global test to screen for defects in the anticoagulant protein C pathway. Thromb Haemost 1996; 75: 562-66.

 

Kraus M, Noah M, Fickenscher K. The PCAT – a simple screening assay for assessing the functionality of the protein C anticoagulant pathway. Thromb Res 1995; 79: 217-22.

 

Meier J, Stocker K. Snake venom protein C activators. In: Handbook of Natural Toxins, Reptile Venoms and Toxins. Tu AT, ed. New York: Marcel Dekker 1990; 265-79.

 

Stocker K, Fischer H, Meier J, Brogli M, Svendsen L. Characterization of the protein C activator Protac    from  the  venom  of  the  southern  copperhead  (Agkistrodon  contortrix)  snake.  Toxicon 1987; 25: 239-52.

Package size:

Vial containing 3 unit/vial Code:

113-01

 

Bulk [unit]

113-04

 

FOR RESEARCH USE ONLY. NOT FOR HUMAN USE OR DRUG USE.

 

Pentapharm 2018年产品列表

 

货号

品名

规格

目录价

品牌

081-01

Pefachrome® TH 5244

25 mg

3000.00

Pentapharm

081-05

Pefachrome® TH 5247

25 mg

3000.00

Pentapharm

081-09

Pefachrome® TH 5251

25 mg

3000.00

Pentapharm

081-11

Pefachrome® FXIIa/TH 5253

25 mg

3000.00

Pentapharm

081-45

Pefachrome® 6017

10 µmol

3000.00

Pentapharm

081-15

Pefachrome® TH 5256

25 mg

3000.00

Pentapharm

081-17

Pefachrome® TG

25 mg

3000.00

Pentapharm

081-66

Pefachrome® TH 8198

25 mg

3000.00

Pentapharm

082-33

Pefachrome® uPA 8294

25 mg

3000.00

Pentapharm

083-01

Pefachrome® PL/Tryp 5261

25 mg

3000.00

Pentapharm

083-02

Pefachrome® PL 5262

25 mg

3000.00

Pentapharm

083-03

Pefachrome® PL 5263

25 mg

3000.00

Pentapharm

083-04

Pefachrome® PL 5264

25 mg

3000.00

Pentapharm

084-01

Pefachrome® TRY 5274

25 mg

3000.00

Pentapharm

085-01

Pefachrome® FXa 5277

25 mg

3000.00

Pentapharm

085-03

Pefachrome® FXa 5279

25 mg

3000.00

Pentapharm

085-06

Pefachrome® FXa/LAL 5288

25 mg

3000.00

Pentapharm

086-11

Pefachrome® LAL 5288

100 μmol

6000.00

Pentapharm

085-27

Pefachrome® FXa 8595

25 mg

3000.00

Pentapharm

802050

Pefachrome® FXa 2732

25 mg

3000.00

Pentapharm

087-03

Pefachrome® C1E

25 mg

3000.00

Pentapharm

089-02

Pefachrome® PCa

25 mg

3000.00

Pentapharm

091-01

Pefachrome® tPA

25 mg

3000.00

Pentapharm

091-03

Pefachrome® tPA5312

25 mg

3000.00

Pentapharm

093-01

Pefachrome® FVIIa

25 mg

3000.00

Pentapharm

095-20

Pefachrome® FIXa

10 μmol

1500.00

Pentapharm

801058

Pefafluor® TH

25 mg

3600.00

Pentapharm

073-70

Aprotinin Conc. Solution (vial 50 ml)

Mio KIU

1500.00

Pentapharm

800277

Aprotinin Powder, lyoph. (vial 1 g)

Mio KIU

1200.00

Pentapharm

126-10

r-Hirudin EC

2000 ATU

3200.00

Pentapharm

381-01

Pefabloc® TH (α-NAPAP)

5 mg

1800.00

Pentapharm

399-01

Pefabloc® SC

1 g

2200.00

Pentapharm

399-01

Pefabloc® SC

10 g

20000.00

Pentapharm

099-01

Pefabloc® FG

1 g

11600.00

Pentapharm

099-11

Pefabloc® FG

50 mg

1200.00

Pentapharm

101-04

Batroxobin maranhao

100 BU

2500.00

Pentapharm

101-06

Batroxobin maranhao

1000 BU

22500.00

Pentapharm

113-01

Protac®

3 U

780.00

Pentapharm

113-05

Protac® solution 10 U/ml

1 ml

2400.00

Pentapharm

116-01

Ecarin

50 EU

1980.00

Pentapharm

119-02

Convulxin

50 µg

8600.00

Pentapharm

121-03

RVV-Factor V Activator

1000 U

6000.00

Pentapharm

121-06

RVV Factor X Activator

5 U

1000.00

Pentapharm

121-07

RVV-Factor X-Activator

50 U

6000.00

Pentapharm

502-01

Pefakit® APC-R Factor V Leiden 3 x 40 tests

Kit

3600.00

Pentapharm

502-21

Pefakit® APC-R Factor V Leiden Controls

Kit

1100.00

Pentapharm

505-01

Pefakit® PiCT®

Kit

4000.00

Pentapharm

505-22

Pefakit® PiCT® Controls UFH

Kit

3900.00

Pentapharm

800186

Pefakit® TAFI

Kit

10000.00

Pentapharm

800187

Pefakit® TAFI Controls and Calibration

Kit

6000.00

Pentapharm

800191

Pefakit® Reptilase® Time

Kit

2800.00

Pentapharm

069-03

Prionex® 10%

100 ml

2000.00

Pentapharm

069-03

Prionex® 10%

500 ml

9000.00

Pentapharm

069-03

Prionex® 10%

1000 ml

12000.00

Pentapharm

801682

Rabbit brain cephalin

100mg

12000.00

Pentapharm

 

 

 

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