pEF1/myc-His C载体说明书

型号 载体名称 出品公司 载体用途 价格
VPI0028 pEF1/myc-His C Invitrogen 哺乳动物表达载体 800



Key Function:Constitutive Expression
Mammalian Selection:Neo,G418
Vector Type:pEF
Cloning Method:Restriction Enzyme/MCS 
Protein Tag or Fusion:c-Myc Epitope Tag,His Tag (6x His) 
Constitutive or Inducible System:Constitutive




pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

  1. Human elongation factor 1?-subunit (hEF-1α) promoter for high-level expression across a broad range of species and cell types (Goldman et al., 1996; Mizushima and Nagata, 1990).
  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc (c-myc) epitope and a polyhistidine metal-binding peptide.
  3. Neomycin resistance gene for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g., COS7).

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.