Merck蛋白抽提产品


Merck蛋白抽提产品

简要描述:BugBuster蛋白抽提试剂用于温和破坏大肠杆菌细胞壁以释放活性蛋白。作为激烈的机械法(如弗式压榨法和超声波法)的替代方法,这种试剂处理起来非常简便、快速,且成本低廉,适合制备表达蛋白用于下游的纯化等应用。

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Merck蛋白抽提

MERCK蛋白抽提

 
1、BugBuster®蛋白抽提试剂——尤其适用于GST融合蛋白的抽提
BugBuster蛋白抽提试剂用于温和破坏大肠杆菌细胞壁以释放活性蛋白。作为激烈的机械法(如弗式压榨法和超声波法)的替代方法,这种试剂处理起来非常简便、快速,且成本低廉,适合制备表达蛋白用于下游的纯化等应用。
 
2、BugBuster Master Mix预混蛋白抽提试剂
BugBuster Master Mix是将BugBuster蛋白抽提试剂,Benzonase核酸酶和rLysozyme™溶菌酶溶液按*化配比混合好的一种非常方便使用的蛋白抽提试剂,有助于在*化条件下获得可溶活性蛋白。
Product
Size
Cat.No.
Price(
BugBuster® Protein Extraction Reagent
100 ml
70584-3
750.00
500 ml
70584-4
3,789.00
BugBuster® Master Mix
100 ml
71456-3
1,700.00
500 ml
71456-4
5,200.00
CytoBuster™ Protein Extraction Reagent
50 ml
71009-3
824.00
250 ml
71009-4
3,122.00

 
 
          
Comparison of E. coli lysis methods
50 ml samples of an induced 500 ml culture of BL21(DE3) containing pET-41a(+) encoding GST were harvested by centrifugation and resuspended in 2 ml 1X PBS, another commercially available protein extraction reagent, or BugBuster Reagent. The sample in PBS was sonicated with 10 pulses at 50% duty for 30 s total. Samples in lysis reagent were treated according to their respective protocols. Extracts were clarified by centrifugation and assayed for GST enzymatic activity.
 
3、Benzonase® 核酸酶——赛多利斯病毒纯化试剂盒推荐使用
应用范围
  •    ² 蛋白抽提操作中降低样品粘度
  •    ² 双向电泳中样品制备过程
  •    ² 重组蛋白样品制备中去除核酸污染
Product
Size
Cat.No.
Price(
Benzonase® Nuclease, Purity > 90%
10 ku
70746-3
1,200.00
2.5 ku
70746-4
400.00
 
 
 
 
 
 
 
Viscosity reduction by Benzonase Nuclease
E. coli BL21(DE3) cells containing a pET construct were suspended in BugBuster Reagent (5 ml/g wet weight). Aliquots of the suspension were treated with the indicated amounts of Benzonase Nuclease for 10 min at