葡聚糖硫酸钠5,000 Sodium Dextran Sulfate 5,000

葡聚糖硫酸钠5,000
Sodium Dextran Sulfate 5,000

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  • 参考文献

葡聚糖硫酸钠5,000葡聚糖硫酸钠5,000                              Sodium Dextran Sulfate 5,000

Sodium Dextran Sulfate 5,000

规格含量 : Total sulfur : 15.0~20.0%
生产商:FUJIFILM Wako Pure Chemical Corporation
保存条件 : 室温
CAS RN® : 9011-18-1 

分子量 : 1,000~9,000

应用级别分子生物学级别,医药生产用原料级别可供选择

包装规格25 g、100 g、500 g 可批量供应


葡聚糖硫酸钠5,000                              Sodium Dextran Sulfate 5,000

◆产品概况


葡聚糖硫酸钠,是一种由葡萄糖聚合,经硫酸化后所得的化合物。本产品的平均分子量约为5000(分子量范围:1000-9000),是聚阴离子物质。在细胞培养中,本品可通过改变细胞表面的电荷,来促使单个细胞悬浮,防止细胞结团,是一种被广泛用于重组蛋白生产的抗结团剂。

本品有分子生物学级别与用于医药生产的CertiPro-L级别两种可供选择。分子生物学级别经电泳或荧光法对DNase和RNase的活性进行检测。CertiPro-L级别是在ISO9001标准下生产的符合日本药典、医药品添加剂规格(药添规)标准的生产专用原料。联系富士胶片和光客服可获取相应产品规格书。

 


◆物性信息


外观:白色~微淡黄色,结晶粉末~粉末

溶解性:易溶于水,几乎不溶于乙醇和丙酮

pH信息:pH(50 g/L,25℃):4.5~8

包装:氩气封装

 


◆用途

可防止细胞培养中的细胞结团,提升CHO细胞等生产用细胞系的重组蛋白产量。


其他用途


1. 诱导小鼠、大鼠等动物的结肠炎模型[1-12]

2. 诱导小鼠的结肠癌模型[13]

3. β-脂蛋白的定量分析中选择性分离脂蛋白[14]

4. 抑制人免疫缺陷病毒(HIV)的体外复制[15]

5. 细胞培养中防止细胞结团;

6. 促进核酸杂交。

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参考文献


 1.

Hori, Y. , Hoshino, J. , Yamazaki, C. , Sekiguchi, T. , Miyauchi, S. , & Mizuno, S. , et al. (1997). Effect of lecithinized-superoxide dismutase on the rat colitis model induced by dextran sulfate sodium. Jpn J Pharmacol, 74(1), 99-103.

DSS 5000溶解于蒸馏水中并制备成3%溶液(25mg/5ml/Kg)【IF=1.305,诱导大鼠结肠炎模型】

 2.

Kawabata, K., Kanmura, S., Morinaga, Y., Tanaka, A., Makino, T., Fujita, T. … Ido, A. (2019). A high‑fructose diet induces epithelial barrier dysfunction and exacerbates the severity of dextran sulfate sodium‑induced colitis. International Journal of Molecular Medicine, 43, 1487-1496.  全文

DSS 5000溶解于蒸馏水中并制备成1%溶液【IF=3.098诱导小鼠结肠炎模型


 3.

Numata, Y. , Tazuma, S. , Nishioka, T. , Ueno, Y. , & Chayama, K. . (2010). Immune response in mouse experimental cholangitis associated with colitis induced by dextran sulfate sodium. Journal of Gastroenterology & Hepatology, 19(8), 910-915.

DSS 5000溶解于蒸馏水中并制备成2.5%溶液【IF=1.83诱导小鼠结肠炎模型


 4.

Ogawa, A., Andoh, Araki, Y., & Bamba, et al. (2004). Neutralization of interleukin-17 aggravates dextran sulfate sodium-induced colitis in mice. CLINICAL IMMUNOLOGY, 110(1), 55-62.

向小鼠喂食含有2.0%(wt/wt) DSS 5000的饲料【IF=3.368诱导小鼠结肠炎模型


 5.

Nishimura, T. , Andoh, A. , Hashimoto, T. , Kobori, A. , Tsujikawa, T. , & Fujiyama, Y. . (2010). Cellobiose prevents the development of dextran sulfate sodium (dss)-induced experimental colitis. Journal of Clinical Biochemistry and Nutrition, 46(2).

向小鼠喂食含有3.5%(wt/wt) DSS 5000的饲料【IF=2.405诱导小鼠结肠炎模型


 6.

Deguchi, Y., Andoh, A., Inatomi, O., Yagi, Y., Bamba, S., Araki, Y., Hata, K., Tsujikawa, T., & Fujiyama, Y. (2007). Curcumin prevents the development of dextran sulfate Sodium (DSS)-induced experimental colitis. Digestive diseases and sciences, 52(11), 2993–2998. 全文

向小鼠喂食含有3.5%(wt/wt) DSS 5000的饲料【IF=2.751诱导小鼠结肠炎模型


 7.

Araki, Y., Mukaisyo, K., Sugihara, H., Fujiyama, Y., & Hattori, T. (2010). Increased apoptosis and decreased proliferation of colonic epithelium in dextran sulfate sodium-induced colitis in mice . Oncology Reports, 24, 869-874.  全文

向小鼠喂食含有5%(wt/wt) DSS 5000的饲料【IF=3.417诱导小鼠结肠炎模型

 8.

Katada, K., Yoshida,N., Suzuki,T., et al. (2008). Dextran sulfate sodium-induced acute colonic inflammation in angiotensin ii type 1a receptor deficient mice. Inflammation Research, 57(2), 84-91.

用饮用水向小鼠喂食3.0 % (w/vol) DSS 5000,持续7天【IF=3.174诱导小鼠结肠炎模型


 9.

Iwanaga, T. , Hoshi, O. , Han, H. , & Fujita, T. . (1994). Morphological analysis of acute ulcerative colitis experimentally induced by dextran sulfate sodium in the guinea pig: some possible mechanisms of cecal ulceration. Journal of Gastroenterology, 29(4), 430.

向豚鼠喂食3.0 % DSS 5000水溶液【IF=6.132诱导豚鼠结肠炎模型


10.

Shintani, N. , Nakajima, T. , Okamoto, T. , Kondo, T. , Nakamura, N. , & Mayumi, T. . (1998). Involvement of cd4+ t cells in the development of dextran sulfate sodium-induced experimental colitis and suppressive effect of igg on their action. General Pharmacology: The Vascular System, 31(3), 477-481.

向小鼠喂食含有5.0 % (w/v) DSS 5000的饮用水,持续7天【IF=1.134诱导小鼠结肠炎模型


11.

Kanauchi, O. , Nakamura, T. , Agata, K. , Mitsuyama, K. , & Iwanaga, T. . (1998). Effects of germinated barley foodstuff on dextran sulfate sodium-induced colitis in rats. Journal of Gastroenterology, 33(2), 179-188.

向大鼠喂食含有3.0%(wt/wt) DSS 5000的饲料【IF=6.132诱导大鼠结肠炎模型


12.

Kao, N. J. , Hu, J. Y. , Wu, C. S. , & Kong, Z. L. . (2016). Curcumin represses the activity of inhibitor-κb kinase in dextran sulfate sodium-induced colitis by s-nitrosylation. International immunopharmacology, 38, 1-7.

向小鼠喂食含有5.0 % (w/v) DSS 5000的饮用水【IF=3.943诱导小鼠结肠炎模型


13.

Gu, S. , Papadopoulou, N. , Gehring, E. M. , Nasir, O. , Dimas, K. , & Bhavsar, S. K. , et al. (2009). Functional membrane androgen receptors in colon tumors trigger pro-apoptotic responses in vitro and reduce drastically tumor incidence in vivo. Molecular Cancer, 8(1), 114.

向小鼠喂食30g/L(即3.0%)的 DSS 5000水溶液,持续7天【IF=15.302诱导小鼠结肠癌模型


14.

Sakagami, T. , & Zilversmit, D. B. . (1961). Separation of dog serum lipoproteins by ultracentrifugation, dextran sulfate precipitation, and paper electrophoresis. Journal of Lipid Research, 2(3), 271-277.

向每毫升血清中加入0.04 ml的5%葡聚糖硫酸盐和0.1 mL的11%氯化钙。在4℃下静置2小时后,将混合物以4,000 X g的速度离心10分钟,去除上清液【IF=4.483选择性分离脂蛋白


15.

Ito, M. , Baba, M. , Sato, A. , Pauwels, R. , Clercq, E. D. , & Shigeta, S. . (1987). Inhibitory effect of dextran sulfate and heparin on the replication of human immunodeficiency virus (hiv) in vitro.Antiviral Research , 7(6), 361-367.

使用PBS溶解(保存于4℃待用)【IF=4.101抑制人免疫缺陷病毒(HIV)的体外复制


16.

Yoshio, A., kira, A., Yoshihide, F., Kazunori, H.,Jin, M., Takafumi, O., Fumiyasu, N., Tadao, B., et al. (2001). Application of 2-aminopyridine fluorescence labeling in the analysis of in vivo and in vitro metabolism of dextran sulfate sodium by size-exclusion high-performance liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 753( 2), 209-215.

0.2mol的 DSS 5000溶解于25mL的氨基吡啶溶液中【利用HPLC分析在体内外的代谢