世界*实验材料供应商 Genlantis Inc. 正式上海金畔为其中国代理， Genlantis Inc. 在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔一直秉承为中国科研客户带来的产品，的服务， Genlantis Inc. 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

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Genlantis位于美国加利福尼亚州的圣地亚哥，是基因治疗系统公司的分公司。Genlantis致力于体内和体外诊断蛋白表达工具的研发及传递技术的开发。Genlantis为的生命科学研究客户生产研发生物学试剂，包括基因传递和质粒DNA的转染试剂（GenePORTER®），哺乳动物细胞siRNA转染试剂（GeneSilencer®），原代神经元细胞的优化转染试剂（NeuroFECT®）和原代神经细胞的转染试剂（NeuroPure®）。

此外，我们还是Tap Express®和Xi-Clone® Instant Enzymeless PCR克隆技术的持有者。这些技术使得科学家们可以同时设计成百上千的蛋白，还可把成本控制在理想的范围内；可以使科学家们由复杂的细菌感染比如疟疾，肺结核，炭疽，天花病毒，衣原体，螺杆菌等快速的制备出相应的蛋白。

• Express toxic proteins
• Optimized E. coli strain for expressing insoluble proteins in soluble form
• Simple and straightforward protocol
• Compatible with all T7 promoter-based expression vectors
• 2 White Papers (See tab above)

Genlantis is pleased to offer SoluBL21™ Competent E. coli**, a significantly improved BL21 host strain for toxic and soluble protein expression. Click on the DATA tab. Using a directed evolution approach, Genlantis scientists have developed a mutant strain of BL21 DE3 E. coli that can produce soluble protein in the majority of cases where expression in the parent BL21(DE3) yielded no detectable soluble product.

Contents

Shipping

The SoluBL21 Chemically Competent E. coli are shipped frozen. 
For maximum stability and long-term use, store the cells and pUC19 control plasmid at -70篊 upon receipt. 
All components are guaranteed stable for 6 months when stored properly

** Patent Pending

Soluble Protein Data

Figure 1: (click to enlarge) Expression of Insoluble Proteins in SoluBL21 vs. standard BL21(DE3) E. coli

Figure 1 legend: A panel of 22 clones that produced little or no soluble protein in standard BL21(DE3) cells were induced side-by-side in BL21(DE3) and SoluBL-21 E. coli. These clones encoded for a wide range of proteins including: cell cycle proteins, ATP-binding proteins, transferases, isomerases, kinases, and repair enzymes from human and other mammalian sources. With the SoluBL21 strain, ~70% of previously insoluble proteins were expressed in soluble form. Examples of SoluBL21-expressed proteins are shown above.

With Genlantis' SoluBL21 strain, a major obstacle to effective protein expression in E. coli has been overcome for many proteins. This significant improvement should enable scientists to make progress in a wide range of applications quicker and far less expensively than in the past.

Toxic Protein Data

We have evaluated the SoluBL21 host to determine whether clones toxic in BL21(DE3) and BL21(DE3)pLysS would exhibit similar toxicity in this slower growing variant. We demonstrate here that in addition to improving the solubility profile of many recombinant proteins, the SoluBL21 strain also permits the establishment of many clones that are toxic to, and cannot be established in, BL21(DE3) and BL21(DE3)pLysS. Furthermore, we show that these toxic clones produce substantial quantities of soluble protein when induced. White Paper Link

Transformation of Plasmid DNAs (Toxic Clones) into standard BL21(DE3), BL21(DE3)pLysS and SoluBL21 E. coli

Soluble Protein Fractions of “Toxic” Clones in SoluBL21 (DE3) E. coli