SYSY 104 211 产品说明书

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Synaptobrevin 2 (VAMP 2)

Major vesicle protein involved in fusion

Fact Sheet Cat. No. 104 211

针对Synaptobrevin2 / VAMP的单克隆小鼠抗体2
Cat. No. 104 211
IgG, unlabeled
50 µg purified IgG, lyophilized. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 10000 up to 1 : 100000 (AP staining) blot 
IP: yes (see remarks) 
ICC: 1 : 1000 image 
IHC: 1 : 500 up to 1 : 1000 image 
IHC-P: 1 : 500 image 
EM: yes 
ELISA: yes (see remarks)
Clone 69.1
Subtype IgG1
Immunogen Synthetic peptide SATAATVPPAAPAGEG (aa 2 – 17 in rat synaptobrevin 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity Human, rat, mouse, hamster, cow; no signal in chicken and zebrafish.
Specificity Specific for VAMP 2, K.O. verified. No cross-reactivity to VAMP 1 and VAMP 3.
Remarks
  • IP: This antibody quantitatively precipitates synaptobrevin 2 from detergent extracts regardless of whether the protein is associated.
  • ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 104 102 or 104 203 as detector antibodies (protocol for sandwich-ELISA).
  • Although the epitope for this antibody is still present in the Botulinumtoxin B cleavage product (aa 1 – 76), it is not recognized for unknown reasons.