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Lens culinaris lectin (LCA/LcH) (10 mg)
Medicago AB Products Are Available Worldwide
For Research Use Only
Code: A05-0104-10mg
Manufacturer’s Part Number: 05-0104-10mg
Shipping Weight: 1.00 pounds
Quantity in Basket: None
Product DescriptionLens culinaris lectin is isolated from lentil seeds by affinity chromatography. It has 2 subunits and a molecular weight of 46 kDa. The lectin gives two major bands in isoelectrofocusing corresponding to the isomers LCA-A and LCA-B. Lens culinaris is not blood group specific. Its carbohydrate specificity is D-mannose and D-glucose. Adding Mn2+ and Ca2+ to the reconstitution buffer will enhance hemagglutination activity. LCA contains a hemagglutinin A and B mixture that agglutinates human red blood cells but that is not group-specific. Lens culinaris agglutinates a 2% suspension of human erythrocytes at a lectin concentration of ≤6 µg/ml in 0.9% NaCl after 2 h at 25°C. Adding 60 mM methylmannoside gives an inhibition with a titer that is at least 16-fold lower than the control. Lens culinaris lectin is supplied as a white lyophilized powder from a buffer containing 1 mM CaCl2, 1 mM MnCI2 and 1 mM MgCI2. No preservatives are added. For laboratory use only. |
Information
| SKU ID# | Packaging | Website Links |
| A05-0104-10mg | 10 mg | www.buffer-solutions。。ｃｏｍ |
| Features | Specifications | Applications |
|
Sugar specificity: α-D-mannose and α-D-glucose. |
Appearance: White lyophilized powder or flocculate. Source: Lentil seeds. Molecular weight: 46 kDa. Sugar specificity: α-D-mannose and α-D-glucose. Activity: Agglutinates human erythrocytes in a 2% blood suspension with lectin concentration ≤8 µg/ml in 0.9% NaCl after 2 h at 25°C. Microorganisms: <100 CFU/g. Protein content: >85%, OD280nm (ε1.6 = 1 mg/ml). Identity: SDS-PAGE, three bands – the three isolectins. Shelf life: >Three years when stored at -20°. |
Hemagglutination. Cell agglutination studies. |
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