世界*实验材料供应商 Applied Genetics Laboratories, Inc 正式上海金畔为其中国代理， appliedgenetics 在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔一直秉承为中国科研客户带来的产品，的服务， appliedgenetics 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获
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Hozier博士在佛罗里达州立大学分子生物物理研究所进行染色体复制研究生工作，并在Tübingen的马克斯·普朗克研究所进行染色体分子组织研究工作，德国。他曾在明尼苏达大学医学院，佛罗里达州立大学佛罗里达州立大学（分子生物物理研究所）和新墨西哥州立大学医学院任教。他于1984年创立了AGL。他的研究兴趣集中在理解染色体畸变和染色体分子组织。他的临床兴趣包括使用DNA探针的高分辨率核型分析和荧光原位杂交，现在通常用于临床工作来定义癌症中的特定染色体重排和发育异常。他是美国医学遗传学院的研究员，并获得美国医学遗传学委员会临床细胞遗传学认证。曾任多所大学和商业机构的临床实验室主任。
Bryan K Hall，MS，CG（ASCP）CM 技术总监 Bryan从佛罗里达理工学院获得细胞和分子生物学硕士学位，对致突变性测定中使用的组织培养系进行染色体分析。他在人类，小鼠，大鼠，猴子等多种物种的细胞遗传学分析和染色体鉴定方面拥有30年经验。布莱恩在约翰霍普金斯大学医学院和密歇根大学儿科遗传学系担任过学术职务，并获得了位于新墨西哥州阿尔伯克基市TriCore参考实验室的临床细胞遗传学经验，负责细胞遗传学实验室的技术和监管工作。除了染色体鉴定，Bryan精通染色体畸变分析，致突变性分析和荧光原位杂交（FISH），包括探针生产。他已经在人类临床组织，建立的细胞系，体细胞杂交体和实验室小鼠的组织培养样品上工作多年。他的分子生物学技术包括核酸纯化，PCR，大插入克隆繁殖和操纵以及基因作图。Bryan曾在AGL工作了8年，目前担任技术总监，他是Cell Line Characterization Studies的研究总监。
Hybloc™ competitor DNA is the repetitive sequence fraction of genomic DNA. Hybloc™ competitor DNA may be effectively substituted for any other competitor DNA (i.e., COT-1 DNA®) for fluorescence in situ hybridization (FISH), Southern blots, microarray technology, or any other protocol requiring competition to prevent repetitive DNA from binding to the target. Hybridization with Hybloc™ competitor DNA shows a marked increase in signal intensity and a significant decrease in the amount of background noise associated with repetitive DNA. Hybloc™ competitor DNA is available for the following species: Mouse, Rat, Human, Bovine, Ovine, Porcine, Chicken, and Canine.
Product: Ovine Hybloc DNA
Catalog #: OHB
Concentration: 1 mg/ml (A260)
Source: Male Ovine Tissues
Storage: -20°C
Description:
Ovine Hybloc DNA is the repetitive sequence fraction of genomic DNA obtained from male ovine tissues by extracting, shearing, denaturing, and reannealing DNA under conditions that enrich for repetitive sequences.
Ovine Hybloc DNA may be effectively substituted for any other competitor DNA (e.g., COT-1 DNA , Salmon Sperm DNA, Genomic DNA, etc.) to suppress cross-hybridization to ovine repetitive sequences for in situ hybridization, Southern blots, microarray technology, or any other protocol requiring competition to block repetitive DNA from binding to the target. Hybridization with Hybloc DNA shows a marked increase in signal intensity and a significant decrease in the amount of background noise associated with repetitive DNA.
How Supplied:
Ovine Hybloc DNAs are supplied in various quantities at 1mg/ml in 10 mM Tris-HCl (pH 7.4), 1 mM EDTA.
Quality Control Assay:
Determination of concentration from the A260 and determination of the A260/A280 ratio (1.8 to 2.0); determination of molecular size: >80% of Ovine Hybloc DNA will run as a broad band between the 50 bp and 600 bp size range.
References:
Landegent JE, Jansen in de Wal N, Dirks RW, Baao F, van der Ploeg M. Use of whole cosmid cloned genomic sequences for chromosomal localization by non-radioactive in situ hybridization. Human Genetics 1987 Dec;77(4):366-70.
Lengauer C, Riethman H, Cremer T. Painting of human chromosomes with probes generated from hybrid cell lines by PCR with Alu and L1 primers. Human Genetics 1990 Nov;86(1):1-6.
Lichter P, Cremer T, Borden J, Manuelidis L, Ward DC. Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Human Genetics 1988 Nov;80(3):224-34.
Lichter P, Tang CJ, Call K, Hermanson G, Evans GA, Housman D, Ward DC. High resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 1990 Jan5;247(4938):64-9.
Scalzi JM, & Hozier, JC. Comparative genome mapping: mouse and rat homologies revealed by fluorescence in situ hybridization. Genomics 1998 Jan1;47(1):44-51.
COT-1 DNA is a registered trademark of Invitrogen Corporation.
部分产品报价如下：
|
货号 |
品名 |
价格 |
品牌 |
|
HB/SF |
0.5mg Hybloc DNA + 5 Human Female Metaphase Slides |
4600 |
Applied Genetics Laboratories |
|
HB/SM |
0.5mg Hybloc DNA + 5 Human Male Metaphase Slides |
4600 |
Applied Genetics Laboratories |
|
BHB |
Bovine Hybloc DNA 0.5mg |
2600 |
Applied Genetics Laboratories |
|
DHB |
Canine Hybloc DNA 0.5mg |
2600 |
Applied Genetics Laboratories |
|
CHB |
Chicken Hybloc DNA 0.5mg |
2600 |
Applied Genetics Laboratories |
|
HFM |
Human Female Metaphase Slides (5/box) |
2600 |
Applied Genetics Laboratories |
|
HHB |
Human Hybloc DNA 0.5mg |
2600 |
Applied Genetics Laboratories |
|
HHB-SS |
Human Hybloc DNA Single-Stranded 1mg |
6933 |
Applied Genetics Laboratories |
|
HMM |
Human Male Metaphase Slides (5/box) |
2600 |
Applied Genetics Laboratories |
|
MHB |
Mouse Hybloc DNA 0.5mg |
2600 |
Applied Genetics Laboratories |
|
OGD |
Ovine Genomic DNA 0.5mg |
2600 |
Applied Genetics Laboratories |
|
OHB |
Ovine Hybloc DNA 0.5mg |
2600 |
Applied Genetics Laboratories |
|
PGD |
Porcine Genomic DNA 0.5mg |
2600 |
Applied Genetics Laboratories |
|
PHB |
Porcine Hybloc DNA 0.5mg |
2600 |
Applied Genetics Laboratories |
|
RHB |
Rat Hybloc DNA 0.5mg |
2600 |
Applied Genetics Laboratories |
|
SHB |
Salmon Hybloc DNA 0.5mg |
2600 |
Applied Genetics Laboratories |
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