现货advancedbiomatrix PureCol®EZ凝胶说明书
PureCol® EZ Gel
Neutralized Type I Collagen Solution, ~5 mg/ml (Bovine)
Catalog #5074
PureCol®EZ凝胶是一种5 mg/ml即用型牛去端胶原溶液，只需加热至37°C即可形成3D水凝胶。该产品为pH中性，采用DMEM/F12培养基等渗配制，可立即添加细胞和3D水凝胶。
产品描述
PureCol®EZ凝胶是一种即用型胶原蛋白溶液，只需在培养箱中加热至37°C即可形成牢固的凝胶。该产品由浓度为0.5%（约5mg/ml）的纯化I型胶原、DMEM/F-12培养基和L-谷氨酰胺和二肽（L-丙氨酸-L-谷氨酰胺）的混合物组成，为细胞培养提供长期的L-谷氨酰胺来源。
PureCol®EZ凝胶旨在通过预配制培养基并将产品调节至中性pH值来提高凝胶的稠度。该产品避免了因试剂添加、pH调节和处理条件的变化而导致的凝胶制备过程中的不一致。
PureCol®EZ凝胶是提供牢固凝胶的理想选择，也可用于制备用于培养细胞的薄层。PureCol®EZ-Gel胶原蛋白采用用户友好的包装，便于使用和储存。本产品是无菌的，作为即用溶液提供。
| Parameter, Testing, and Method | PureCol® EZ Gel Type I Collagen #5074 |
| Sterilization Method | Filtration |
| Extraction Method | Enzyme – atelocollagen |
| Form | Solution |
| Package Size | 35 mL |
| Storage Temperature | 2-10°C |
| Shelf Life | Minimum of 6 months from date of receipt |
|
Collagen Concentration – Biuret |
~5 mg/mL |
|
Collagen Purity – Silver Staining |
>99% |
| pH | 6.9-7.4 |
| Kinetic Gel Test (Minutes) | <40 |
| Gel Formation Tube Test (Minutes) | <40 |
|
Fibrillogenesis (Absorbance Units) |
>0.5 |
|
Electrophoretic Pattern – Coomassie Blue |
Characteristic |
| Sterility – USP modified | No growth |
| Endotoxin – LAL | <1.0 EU/mL |
| Osmolality (mOsmo H2O/kg) | 300-360 |
| Cell Attachment Assay | Pass |
| Source | Bovine Hide |
| Hydrogel Young's Modulus E (Pa) | Characteristic |
| Medium Supplement | DMEM/F-12 |
| L-Glutamine Source | Mixture of L-Glutamine and Dipeptide (L-alanine-L-glutamine) |