advancedbiomatrix TeloCol®-3说明书
TeloCol®-3
Type I Collagen Solution, 3 mg/ml (Bovine)
TeloCol-3是50mL的3mg/mL的I型牛telocollagen溶液，用于2D涂层或3D水凝胶。该套件配有中和溶液，便于操作。TeloCol-3作为端羟基胶原，将形成更牢固的水凝胶，并且凝胶速度明显快于相同浓度的端羟基胶原溶液。
产品描述
TeloCol®-3牛胶原蛋白溶液来源于酸提取过程，产生端肽完整胶原蛋白。TeloCol®是溶于0.01 N HCL的端羟基胶原。胶原链两端的端肽区，即N-端肽区和C-端肽区保持不变。
TeloCol®-3胶原蛋白约为95%的I型胶原蛋白，其余为III型胶原蛋白。每个产品包括一瓶含有50ml胶原蛋白溶液的瓶子，以及一瓶预先配制的用于形成胶原蛋白凝胶的中和溶液。该产品的供应浓度约为3mg/ml。TeloCol®-3经过无菌过滤，并以方便使用和储存的包装提供。
TeloCol®-3是表面涂层的理想选择，为培养细胞提供薄层准备，或用作固体凝胶。
| Parameter, Testing, and Method | TeloCol®-3 Type I Collagen #5026 |
| Sterilization Method | Filtration |
| Extraction Method | Acid – telocollagen |
| Form | Solution |
| Package Size | 50 mL Kit |
| Storage Temperature of Collagen | 2-10°C |
| Storage Temperature of Neutralization Solution | Room Temperature |
| Shelf Life | Minimum of 6 months from date of receipt |
|
Collagen Concentration – Biuret |
2.8-3.3 mg/mL |
|
Collagen Purity – Silver Staining |
>99% |
| pH | 1.9-3.0 |
| Kinetic Gel Test (Minutes) | <40 |
| Gel Formation Tube Test (Minutes) | <40 |
|
Fibrillogenesis (Absorbance Units) |
>0.35 |
|
Electrophoretic Pattern – Coomassie Blue |
Characteristic |
| Sterility – USP modified | No growth |
| Endotoxin – LAL | <10.0 EU/mL |
| Osmolality (mOsmo H2O/kg) | <35 |
| Cell Attachment Assay | Pass |
| Source | Bovine Hide |
| Hydrogel Young's Modulus E (Pa) | Characteristic |