日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody

日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody
中文名称:抗丙二醛单克隆抗体
英文名称:Anti Malondialdehyde(MDA) monoclonal antibody
货号:MMD-030n
规格:30ug
Anti MDA monoclonal antibody  脂质过氧化生物标志物
适用于免疫组化(仅供研究使用)
About malondialdehyde (MDA)
Malondialdehyde (MDA) is one of the major aldehyde derive from lipid peroxidation. MDA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with MDA and lysine residue leads to the formation of numerous numbers of adducts, such as dihydropyridine-lysine (DHP-lysine) type derivative. This monoclonal antibody is specific for the MDA-modified protein, especially DHP-lysine type derivative.

关于丙二醛(MDA)
丙二醛(MDA)是衍生自脂质过氧化的主要醛之一。 MDA是高度反应性的醛,并与蛋白质中的赖氨酸残基反应。 与MDA和赖氨酸残基的反应导致众多数量的加合物的形成,例如二氢吡啶-赖氨酸(DHP-赖氨酸)型衍生物。 该单克隆抗体对MDA修饰的蛋白质,特别是DHP-赖氨酸型衍生物是特异性的。
Specifications

Clone #: 1F83
Antigen: MDA-modified keyhole-lympet hemocyanine.
Form: Frozen (100 碌g/mL antibody in 10mM PBS containing 0.1% NaN3 and 0.5% BSA). Purified by Protein-A.
Application: Immunohistochemistry.
Recommended antibody concentration is 0.5-1.0 碌g/mL on paraformaldehyde fixed tissue.
Specificity: MSpecific for MDA-modified protein (especially DHP-lysine).
Subclass: Mouse IgG2a(lambda)
Storage: Less than -20掳C


Specificity of anti MDA antibody.

Immunohistochemical detection of MDA-modified protein in atherosclerotic aorta.
Dr. N Shibata, Tokyo Women’s Medical University.
技术参数
克隆号#:1F83;
抗原:MDA修饰的钥孔血蓝蛋白(KLH);
性状:冻结(100μg/mL抗体在含有0.1%NaN3和0.5% BSA的10mM PBS中),经过蛋白A纯化;
应用:免疫组织化学。对多聚甲醛固定的组织推荐抗体浓度为0.5-1.0μg/mL;
特异性:特异性针对MDA修饰的蛋白(特别是DHP-赖氨酸)。
亚基:小鼠IgG2a(lambda)
储存:小于-20℃
其他相关产品:
·  抗丙烯醛单克隆抗体 Anti Acrolein(ACR) monoclonal antibody
·  抗丙烯醛单克隆抗体 Anti Acrolein(ACR) monoclonal antibody
·  抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
·  己酰-赖氨酸加合物ELISA试剂盒 Hexanoyl-Lysine adduct(HEL) ELISA Kit
References 参考文献

1) Immunochemical detection of a lipofuscin-like fluorophore derivered from malondialdehyde and lysine.
S Yamada, S Kumazawa, T Ishii, T Nakayama, K Itakura, N Shibata, M Kobayashi, K Sakai, T Osawa and K Uchida.
J.Lipid Res. 42, p1187-1196 (2001)
2) Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress.
Muratori M, Tamburrino L, Marchiani S, Cambi M, Olivito B, Azzari C, Forti G, Baldi E
Mol Med. 21,p109-122(2015). doi: 10.2119/molmed.2014.00158.

日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody技术资料:http://www.jaica.com/e/technical_forum_nof.html#MDA
日本 JaICA 老化Anti Malondialdehyde(MDA) monoclonal antibody 说明书:
http://www.jaica.com/e/pdf/mda_ab_manual.pdf

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