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LBIS® 小鼠卵清蛋白特异性免疫球蛋白E（OVA-IgE）ELISA试剂盒 LBIS® OVA-IgE Mouse

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LBIS® 小鼠卵清蛋白特异性免疫球蛋白E（OVA-IgE）ELISA试剂盒
LBIS® OVA-IgE Mouse

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

LBIS® 小鼠卵清蛋白特异性免疫球蛋白E（OVA-IgE）ELISA试剂盒 LBIS® OVA-IgE Mouse小鼠卵清蛋白特异性免疫球蛋白E（OVA-IgE） ELISA试剂盒

 


　　IgE（Immunoglobulin E，免疫球蛋白E）是第五个被发现的免疫球蛋白，由5个结构域（VH、CHε 1～4）构成的两个Hε链和两个L链中组成的IgE，其分子量约190000，电泳实验中向γ1域移动。IgE的代谢半衰期约3天，正常人血清中的IgE浓度非常低，约300 ng/mL。但在寄生虫感染和枯草热时浓度会升高。与过敏性相关的IgE被称作反应素。因接触过敏原导致反应素含量升高，反应素会在Fc域与存在于皮肤、呼吸道、消化脏器中嗜碱性粒细胞和肥大细胞的FcεR1受体结合，引起细胞过敏。在结合过敏原后细胞发生脱粒现象，组胺，五羟色胺，蛋白酶，肝素，趋化因子，前列腺素，白三烯等被投放，经过支气管收缩和黏膜水肿，分泌亢进，从而诱发支气管哮喘，部分荨麻疹，过敏性鼻炎，过敏性反应等I型过敏性反应。

　　本试剂盒是以OVA（卵清蛋白）作为免疫的抗原，在一个简化的反应系统中通过测定鼠抗OVA- IgE抗体值来进行小鼠免疫系统检测的试剂盒。

◆特点

LBIS® 小鼠卵清蛋白特异性免疫球蛋白E（OVA-IgE）ELISA试剂盒 LBIS® OVA-IgE Mouse


●　测定时间短（总反应时间：1小时50分钟）。

●　微量样本即可测定。

●　使用无害的防腐剂。

●　全部试剂为溶液即用类型。

●　高测定精度和高重复性。

●　操作简便，无需进行特殊前处理。

 

◆试剂盒组成


组成品

状态

包装

OVA包被96孔板（干燥板）

清洗后使用

96 wells（8×12）／1个

标准溶液（Anti OVA-IgE:1,200 U/mL）（单抗）

稀释后使用

100 μL/1瓶

缓冲液

直接使用

60 mL/1瓶

生物素结合抗小鼠IgE抗体（单抗）

稀释后使用

200 μL/1瓶

过氧化物酶·抗生素结合物

稀释后使用

200 μL/1瓶

显色液（TMB）

直接使用

12 mL/1瓶

终止液（1M H2SO4）※小心轻放

直接使用

12 mL/1瓶

浓缩清洗液（10×）

稀释后使用

100 mL/1瓶

孔板密封膜

3个

产品说明书

1本

 


◆样本信息


●　小鼠血清·血浆

●　10 μL/well（稀释样本）

※　样本需要用试剂盒附带的缓冲液调至标准曲线范围内。

※　样本必须稀释10倍以上。

 


◆测定范围


1.88～120 U/mL（标准曲线范围）

（本试剂盒中1 U/mL定义为抗原结合常数（Ka）为2.0×108 M-1的抗体1.3 ng/mL）

 


◆实验数据


精度测试（组内变异）


样本

A

B

1

70.7

19.1

2

71.0

18.7

3

77.1

19.6

4

74.3

19.7

5

72.9

18.9

Mean

73.2

19.2

SD

2.6

0.42

CV（%）

3.6

2.2

单位：U/mL



重复性测试（组间变异）


测定日/样本

C

D

E

0天

60.0

15.0

3.75

1天

59.1

15.0

3.75

2天

58.1

14.7

3.65

3天

63.6

16.0

3.48

Mean

60.2

15.2

3.66

SD

2.4

0.57

0.13

CV（%）

4.0

3.7

3.4

单位：U/mL



加标回收测试


样本F


添加量

实测值

回收量

回收率（%）

0.00

6.93

—

—

5.35

12.2

5.27

98.5

10.7

17.8

10.9

102

17.1

25.1

18.2

106

单位：U/mL，n=3


样本G


添加量

实测值

回收量

回收率（%）

0.00

40.5

—

—

30.8

70.4

29.5

95.8

35.9

77.1

36.6

102

53.9

94.9

54.4

101

单位：U/mL，n=3



稀释直线性测试


2个血清样本连续用稀释缓冲液稀释3个梯度测定结果，直线回归值R2=0.9987~0.9999

参考文献



 1.

Pinocembrin attenuates allergic airway inflammation via inhibition of NF-κB pathway in mice. Gu X, Zhang Q, Du Q, Shen H, Zhu Z. Int Immunopharmacol. 2017 Oct 18;53:90-95.

 2.

A dichloromethane fraction of Triticum aestivum sprouts reduces allergic immune response through inhibiting Th2 differentiation in ovalbumin‑immunized mice. Ki HH, Hwang SW, Lee JH, Kim YH, Kim DK, Lee YM. Mol Med Rep. 2017 Sep;16(3):3535-3541.

 3.

Urban PM2.5 exacerbates allergic inflammation in the murine lung via a TLR2/TLR4/MyD88-signaling pathway. He M, Ichinose T, Yoshida Y, Arashidani K, Yoshida S, Takano H, Sun G, Shibamoto T. Sci Rep. 2017 Sep 8;7(1):11027.

 4.

Activation of group 2 innate lymphoid cells exacerbates and confers corticosteroid resistance to mouse nasal type 2 inflammation. Morikawa T, Fukuoka A, Matsushita K, Yasuda K, Iwasaki N, Akasaki S, Fujieda S, Yoshimoto T. Int Immunol. 2017 May 1;29(5):221-233.


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Aquaporin-3 potentiates allergic airway inflammation in ovalbumin-induced murine asthma. Ikezoe K, Oga T, Honda T, Hara-Chikuma M, Ma X, Tsuruyama T, Uno K, Fuchikami J, Tanizawa K, Handa T, Taguchi Y, Verkman AS, Narumiya S, Mishima M, Chin K. Sci Rep. 2016 May 11;6:25781.

 6.

Exposure to bisphenol A enhanced lung eosinophilia in adult male mice. He M, Ichinose T, Yoshida S, Takano H, Nishikawa M, Shibamoto T, Sun G. Allergy Asthma Clin Immunol. 2016 Apr 14;12:16.

 7.

Differences in allergic inflammatory responses between urban PM2.5 and fine particle derived from desert-dust in murine lungs. He M, Ichinose T, Kobayashi M, Arashidani K, Yoshida S, Nishikawa M, Takano H, Sun G, Shibamoto T. Toxicol Appl Pharmacol. 2016 Apr 15;297:41-55.

 8.

Desert dust induces TLR signaling to trigger Th2-dominant lung allergic inflammation via a MyD88-dependent signaling pathway. He M, Ichinose T, Song Y, Yoshida Y, Bekki K, Arashidani K, Yoshida S, Nishikawa M, Takano H, Shibamoto T, Sun G. Toxicol Appl Pharmacol. 2016 Apr 1;296:61-72.

 9.

Administration of Pigment Epithelium-Derived Factor Inhibits Airway Inflammation and Remodeling in Chronic OVA-Induced Mice via VEGF Suppression. Zha W, Su M, Huang M, Cai J, Du Q. Allergy Asthma Immunol Res. 2016 Mar;8(2):161-9.

10.

Low-dose benzo[a]pyrene aggravates allergic airway inflammation in mice. Yanagisawa R, Koike E, Win-Shwe TT, Ichinose T, Takano H. J Appl Toxicol. 2016 Feb 25.

11.

Prevention of allergic rhinitis by ginger and the molecular basis of immunosuppression by 6-gingerol through T cell inactivation. Kawamoto Y, Ueno Y, Nakahashi E, Obayashi M, Sugihara K, Qiao S, Iida M, Kumasaka MY, Yajima I, Goto Y, Ohgami N, Kato M, Takeda K. J Nutr Biochem. 2016 Jan;27:112-22.

12.

Immunotoxic Effect of Low-Dose Methylmercury Is Negligible in Mouse Models of Ovalbumin or Mite-Induced Th2 Allergy. Nakamura R, Takanezawa Y, Sone Y, Uraguchi S, Sakabe K, Kiyono M.

Biol Pharm Bull. 2016;39(8):1353-8.

13.

Prevention of allergic rhinitis by ginger and the molecular basis of immunosuppression by 6-gingerol through T cell inactivation. Yoshiyuki Kawamoto, Yuki Ueno, Emiko Nakahashi, Momoko Obayashi, Kento Sugihara, Shanlou Qiao, Machiko Iida, Mayuko Y. Kumasaka, Ichiro Yajima, Yuji Goto, Nobutaka Ohgami, Masashi Kato, Kozue Takeda. The Journal of Nutritional Biochemistry, Volume 27, Jan. 2016, Pages 112–122

14.

Effects of Sohamhyoong-Tang on Ovalbumin-Induced Allergic Reaction in BALB/c Mice. Jo SH, Lee YJ, Kang DG, Lee HS, Kim DK, Park MC. Evid Based Complement Alternat Med. 2016;2016:6286020.

15.

Effect of diosmetin on airway remodeling in a murine model of chronic asthma. Ge A, Liu Y, Zeng X, Kong H, Ma Y, Zhang J, Bai F, Huang M. Acta Biochim Biophys Sin (Shanghai). Vol.47(8), p604-11, Aug 2015.

16.

PM2.5-rich dust collected from the air in Fukuoka, Kyushu, Japan, can exacerbate murine lung eosinophilia. He M, Ichinose T, Ren Y, Song Y, Yoshida Y, Arashidani K, Yoshida S, Nishikawa M, Takano H, Sun G. Inhal Toxicol. Vol.27(6), p287-99, May 2015.

17.

Anti-asthma potential of crocin and its effect on MAPK signaling pathway in a murine model of allergic airway disease. Xiong Y, Wang J, Yu H, Zhang X, Miao C. Immunopharmacol Immunotoxicol. Vol.10, p1-8, Mar 2015.

18.

Roles of lipoxin A4 receptor activation and anti-interleukin-1β antibody on the toll-like receptor 2/mycloid differentiation factor 88/nuclear factor-κB pathway in airway inflammation induced by ovalbumin. Kong X, Wu SH, Zhang L, Chen XQ. Mol Med Rep. 2015 Mar 5

19.

Pharyngeal aspiration of metal oxide nanoparticles showed potential of allergy aggravation effect to inhaled ovalbumin. Horie M, Stowe M, Tabei M, Kuroda E. Inhal Toxicol. Vol.27(3), p181-90, Feb 2015.

20.

Oxidized dietary oils enhance immediate- and/or delayed-type allergic reactions in BALB/c mice. Ogino H, Sakazaki F, Okuno T, Arakawa T, Ueno H. Allergol Int. Vol.64(1), p66-72, Jan 2015.

21.

The effects of nodakenin on airway inflammation, hyper-responsiveness and remodeling in a murine model of allergic asthma. Xiong Y, Wang J, Yu H, Zhang X, Miao C, Ma S. Immunopharmacol Immunotoxicol. Vol.36(5), p341-348, Oct 2014.

22.

Allogeneic pluripotent stem cells suppress airway inflammation in murine model of acute asthma. Ogulur I, Gurhan G, Kombak FE, Filinte D, Barlan I, Akkoc T. International Immunopharmacology, Vol.22(1), p31-40 Sep 2014.

23.

Effects of prior oral exposure to combinations of environmental immunosuppressive agents on ovalbumin allergen-induced allergic airway inflammation in Balb/c mice. Fukuyama T, Nishino R, Kosaka T, Watanabe Y, Kurosawa Y, Ueda H, Harada T.. Immunopharmacol Immunotoxicol. Vol.36(4), p261-70, Aug 2014.

24.

Enhancement of OVA-induced murine lung eosinophilia by co-exposure to contamination levels of LPS in Asian sand dust and heated dust. Ren Y, Ichinose T, He M, Song Y, Yoshida Y, Yoshida S, Nishikawa M, Takano H, Sun G, Shibamoto T. Allergy, Asthma & Clinical Immunology, Vol.10(1), Jun 2014.

25.

A bacterial extract of OM-85 Broncho-Vaxom prevents allergic rhinitis in mice. Han L, Zheng CP, Sun YQ, Xu G, Wen W, Fu QL. American Journal of Rhinology & Allergy, Vol.28(2), p110-116, Mar-Apr 2014.

26.

Broncho-Vaxom Attenuates Allergic Airway Inflammation by Restoring GSK3β-Related T Regulatory Cell Insufficiency. Fu R, Li J, Zhong H, Yu D, Zeng X, Deng M, Sun Y, Wen W, Li H. PLoS One. 2014 Mar 25;9(3):e92912

27.

Lung inflammation by fungus, Bjerkandera adusta isolated from Asian sand dust (ASD) aerosol and enhancement of ovalbumin-induced lung eosinophilia by ASD and the fungus in mice. Liu B, Ichinose T, He M, Kobayashi F, Maki T, Yoshida S, Yoshida Y, Arashidani K, Takano H, Nishikawa M, Sun G, Shibamoto T. Allergy, Asthma & Clinical Immunology, Vol.10(1), Feb 2014.

28.

Midazolam inhibits IgE production in mice via suppression of class switch recombination. Kusama H, Kobayashi R, Kurita-Ochiai T. Journal of Oral Science, Vol.56(1), p77-83, 2014.

29.

Induction of immune tolerance and reduction of aggravated lung eosinophilia by co-exposure to Asian sand dust and ovalbumin for 14 weeks in mice. He M., Ichinose T., Yoshida S., Takano H., Nishikawa M., Sun G. and Shibamoto T. Allergy, Asthma & Clinical Immunology, Vol.9(19), 2013.

30.

Galangin Abrogates Ovalbumin-Induced Airway Inflammation via Negative Regulation of NF-B. Zha W-J., Qian Y., Shen Y., Du Q., Chen F-F., Wu Z-Z., Li X. and Huang M. Evidence-Based Complementary and Alternative Medicine, Vol.2013 (2013), p14.

31.

Effects of two Asian sand dusts transported from the dust source regions of Inner Mongolia and northeast China on murine lung eosinophilia. M.He, T.Ichinose, Y.Song, Y.Yoshida, K.Arashidani, S.Yoshida, B.Liu, M.Nishikawa, H.Takano, G.Sun. Toxicology and Applied Pharmacology, Available online 26 July 2013.

32.

Effect of the size of receptor in allergy detection using field effect transistor biosensor. S.Hideshima, S.Kuroiwa, M.Kimura, S.Cheng, T.Osaka. Electrochimica Acta, Available online 24 July 2013.

33.

Elevated Macrophage Inflammatory Protein 1α and Interleukin-17 Production in an Experimental Asthma Model Infected with Respiratory Syncytial Virus. T.Ishioka, Y.Yamada, H.Kimura, M.Yoshizumi, H.Tsukagoshi, K.Kozawa, K.Maruyama, Y.Hayashi, M.Kato. Int Arch Allergy Immunol, Vol.161(suppl 2), p129-137, May 2013.

34.

Leukotriene B4 receptor BLT2 negatively regulates allergic airway eosinophilia . Y.Matsunaga, S.Fukuyama, T.Okuno, F.Sasaki, T. Matsunobu, Y.Asai, K.Matsumoto, K.Saeki, M.Oike, Y.Sadamura, K.Machida, Y.Nakanishi, M.Kubo, T.Yokomizo and H.Inoue. The FASEB Journal, Published online before print April 19, 2013.

35.

Effects of exposure to nanoparticle-rich or -depleted diesel exhaust on allergic pathophysiology in the murine lung. Tanaka M., Aoki Y., Takano H., Fujitani Y., Hirano S., Nakamura R., Sone Y., Kiyono M., Ichinose T., Itoh T., Inoue K. Journal of Toxicological Sciences, Vol.38(1), p35-48, Feb 2013.

36.

HIF-1α Inhibition Reduces Nasal Inflammation in a Murine Allergic Rhinitis Model. Zhou H, Chen X, Zhang W-M, Zhu L-P, Cheng L. PLOS one, 2012.

37.

Human Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Prevent Allergic Airway Inflammation in Mice. Sun Y-Q, Deng M-X, He J, Zeng Q-X, Wen W, Wong D S.H, Tse H-F, Xu G, Lian Q, Shi J, Fu Q-L. STEM CELLS, Vol.30(12), p2692-2699, Dec 2012.

38.

Aggravating effects of Asian sand dust on lung eosinophilia in mice immunized beforehand by ovalbumin. He M, Ichinose T, Yoshida S, Takano H, Nishikawa M, Mori I, Sun G, Shibamoto T. Inhalation Toxicology, Vol.24(11) , p751-761, Sep 2012.

39.

Attenuation of airway hyperreactivity and T helper cell type 2 responses by coumarins from Peucedanum praeruptorum Dunn in a murine model of allergic airway inflammation. Xiong Y-Y, Wu F-H, Wang J-S, Li J, Kong L-Y. Journal of Ethnopharmacology, Vol.141(1), p314-321, May 2012.

40.

Effects of lysed Enterococcus faecalis FK-23 on experimental allergic rhinitis in a murine model. Zhu L,Shimada T, Chen R, Lu M, Zhang Q, Lu W, Yin M, Enomoto T, Cheng L. Journal of Biomedical Research, Vol.26(3), p226-234, May 2012.

41.

Sphingosine-kinase 1 and 2 contribute to oral sensitization and effector phase in a mouse model of food allergy. S. C. Diesner., A. Olivera., S. Dillahunt., C. Schultz., T. Watzlawek., E. Forster-Waldl., A. Pollak., E. Jensen-Jarolim., E. Untersmayr., J. Rivera. Immunology Letters, Vol. 141, Issue 2, 30 January 2012, Pages 210-219

42.

Identification of Semaphorin 4B as a Negative Regulator of Basophil-Mediated Immune Responses. Y. Nakagawa., H. Takamatsu., T. Okuno., S. Kang., S. Nojima., T. Kimura., T. R. Kataoka., M. Ikawa., T. Toyofuku., I. Katayama., and A. Kumanogoh. The Journal of Immunology, March 1, 2011 vol. 186 no. 5 2881-2888

43.

Suppression of ovalbumin-induced allergic diarrhea by diminished intestinal peristalsis in RAMP1-deficient mice. R. Yoshikawa., N. Mikami., I. Otani., T. Kishimoto., S. Nishioka., N. Hashimoto., Y. Miyagi., Y. Takuma., K. Sueda., S. Fukada., H. Yamamoto., K. Tsujikawa. Biochemical and Biophysical Research Communications, Vol. 410, Issue 3, 8 July 2011, Pages 389-393

44.

Cortex Mori Radicis extract exerts antiasthmatic effects via enhancement of CD4+CD25+Foxp3+ regulatory T cells and inhibition of Th2 cytokines in a mouse asthma model. H.-J. Kim., H. J. Lee., S.-J. Jeong., H.-J. Lee., S.-H. Kim., E.-J. Park. Journal of Ethnopharmacology, Vol. 138, Issue 1, 31 October 2011, Pages 40-46

45.

Urban particulate matter in Beijin,China,enhances allergen-induced murine lung eosinophilia. He,M.,Ichinose,T.,Yoshida,S.,Nishikawa,M.,Mori,I.,Yanagisawa,R.,Takano,H.,Inoue,K.,Sun,G.,Shibamoto,T. Inhalation Toxicology 22(9):709-718,August,2010

46.

Deficiency in the Serum-Derived Hyaluronan-Associated Protein-Hyaluronan Complex Enhances Airway Hyperresponsiveness in a Murine Model of Asthma. L. Zhu., L. Zhuo., K. Kimata., E. Yamaguchi., H. Watanabe., M. A. Aronica., V. C. Hascall., K. Baba. Int Arch Allergy Immunol 153:223-233 2010

47.

Thioredoxin suppresses airway inflammation independently of systemic Th1/Th2 immune modulation. M. Torii., L. Wang., N. Ma., K. Saito., T. Hori., M. Sato-Ueshima., Y. Koyama., H. Nishikawa., N. Katayama., A. Mizoguchi., H. Shiku., J. Yodoi., K. Kuribayashi., T. Kato. European Journal of Immunology Vol.40(3) 787-796 2010

48.

Peritoneal injection of fucoidan suppresses the increase of plasma IgE induced by OVA-sensitization. Yanase,Y.,Hiragun,T.,Uchida,K.,Ishii,K.,Oomizu,S.,Suzuki,H.,Mihara,S.,Iwamoto,K.,Matsuo,H.,Onishi,N.,Kameyoshi,Y.,and Hide,M. Biochemical and Biophysical Research Communications 387:3:435- 439,2009

49.

Peroxisome Proliferator-Activated Receptor gNegatively Regulates Allergic Rhinitis in Mice. Fukui,N.,Honda,K.,Ito,E.,and Ishikawa K. Allergology Internatioal.58:247-253,2009

50.

IL-16 Variabillity and Modulation by Antiallergic Drugs in a Murine Experimental Allergic Rhinitis Model. Akiyama,K.,Karaki,M.,Kobayshi,R.,Dobashi,H.,Ishida,T, and Mori,N. Allergy and Immunology 149:4,2009

51.

Frequency of Foxp3+CD4+CD25+ T cell is associated with the phenotypes of allergic asthma. Matsumoto,K.,Inoue,H.,Fukuyama,A.,Kan,O,K.,Eguchi,T,M.,Matsumoto,T.,Moriwaki,A.,Nakano,T., and Nakanishi,Y. Respirology 14:2,2009

52.

Differential Regulatory Function of Resting and Preactivated Allergen-Specific CD4+CD25+ Regulatory T Cells in Th2-Type Airway Inflammation. Saito, K., Torii, M., Ning Ma, Tsuchiya, T., Wang, L., Hori, T., Nagakubo, D., Nitta, N.,Kanegasaki, S., Hieshima, K., Yoshie, O., Gabazza, E.C., Katayama, N., Shiku, H.,Kuribayashi, K. and Kato, T. The Journal of Immunology, 181:6889-6897, 2008

53.

Effects of Asian Sand Dust, Arizona Sand Dust, Amorphous Silica and Aluminum Oxide on Allergic Inflammation in the Murine Lung. Ichinose, T., Yoshida, S., Sadakane, K., Takano, H., Yanagizawa, R., Inoue, K., Nishikawa, M.,Mori, I., Kawazato, H., Yasuda, A. and Shibamoto, T. Inhalation Toxicology, Volume 20, Issue 7, 685-694, 2008

54.

The Effects of Microbial Materials Adhered to Asian Sand Dust on Allergic Lung Inflammation. Ichinose,T., Yoshida,S., Hiyoshi,K., Sadakane,K., Takano,H., Nishikawa,M., Mori,I., Yanagisawa,R., Kawazato,H., Yasuda,A., and Shibamoto,T. Arch Environ Contam Toxicol 55:348-357,2008

55.

Differential Regulatory Function of Resting and Preactivated Allergen-Specific CD4+CD25+ Regulatory T cell in Th2-Type Airway Inflammation. Saito,K., Torii,M., Ma,N., Tsuchiya,T., Wang,L.,Hori,T., Nagakubo,D., Nitta,N., Kanegasaki,S., Hieshima,K., Yoshie,O., Gabazza,E,C., Katayama,N., Shiku,H., Kuribayashi,K., and Kato,T. The Journal of Immunology 181:6889-6897,2008

产品列表
产品编号 产品名称 产品规格 产品等级 备注
639-07651 （AKRIE-030）小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE) ELISA试剂盒
 LBIS® OVA-IgE Mouse		 96 tests – –

临床前研究用检测试剂 免疫·过敏研究领域

发表于由

临床前研究用检测试剂
免疫·过敏研究领域

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

临床前研究用检测试剂 免疫·过敏研究领域




◆免疫球蛋白E（IgE）

生产商编号
和光编号		 产品名称 检测范围

检测样本量

（稀释样本）

AKRIE-010

633-02899

 LBIS® IgE Mouse ELISA Kit  
小鼠免疫球蛋白E（IgE） ELISA试剂盒 		 1～100 ng/mL 50 μL
AKRIE-011
636-04349

LBIS® IgE Rat ELISA Kit  
大鼠免疫球蛋白E（IgE）ELISA试剂盒

 1～100 ng/mL 50 μL


◆白细胞介素（Interleukin）

生产商编号

和光编号

 产品名称 检测范围 检测样本量
（稀释样本）
AKMIL12-011
638-40841		 LBIS® Mouse IL-12 ELISA   Kit
小鼠白细胞介素-12（IL-12）ELISA试剂盒		 2.87～700 pg/mL 100 μL

◆TDAR(T cell Dependent Antibody Responce)试验用


生产商编号
和光编号		 产品名称 检测范围 检测样本量
（稀释样本）
AKRKG-010
632-13751

LBIS® KLH(TDAR)-Rat IgG ELISA KIT
血蓝蛋白（KLH）（T细胞依赖性抗原）

大鼠免疫球蛋白G（IgG） ELISA试剂盒 (＊１)

 0.47～30 ng/mL 50 μL
AKRKM-010
639-13761

LBIS® KLH(TDAR)-Rat IgM ELISA KIT
血蓝蛋白（KLH）（T细胞依赖性抗原）

大鼠免疫球蛋白M（IgM） ELISA试剂盒 (＊２)

 3.13～200 ng/mL 50 μL


(＊1) 为了避免非特异性反应，请将检测样本稀释500倍以上

(＊2) 为了避免非特异性反应，请将检测样本稀释200倍以上




◆抗OVA(ovalbumin)抗体

生产商编号
和光编号		 产品名称 检测范围 检测样本量
（稀释样本）
AKRIE-030
639-07651

LBIS® OVA-IgE Mouse
小鼠卵清蛋白特异性免疫球蛋白E

（OVA-IgE）ELISA试剂盒 

 1.88～120 U/mL 10 μL
AKRIE-040
636-07661

LBIS® OVA-IgG1 Mouse
小鼠卵清蛋白特异性免疫球蛋白G1

（OVA-IgG1）ELISA试剂盒

 1.88～120 U/mL 10 μL




◆抗核酸抗体


生产商编号
和光编号		 产品名称 检测范围 检测样本量
（稀释样本）
AKRDD-061
637-02691		 LBIS® Anti dsDNA-Mouse ELISA Kit
小鼠抗双链DNA抗体 ELISA试剂盒		 15.6～1000mU/mL 100 μL

AKRSD-051
630-02701

 LBIS® Anti ssDNA-Mouse ELISA Kit
LBIS® 抗ssDNA-小鼠ELISA试剂盒 		 15.6～1000mU/mL 100 μL

◆类风湿因子


生产商编号
和光编号		 产品名称 检测范围 检测样本量
（稀释样本）
AKRRG-101
637-02679

LBIS® Rheumatoid Factor IgG-Mouse ELISA Kit
小鼠免疫球蛋白G（IgG）

类风湿因子 ELISA试剂盒

 15.6～1000 mU/mL 100 μL
AKRRG-111
634-02689

LBIS® Rheumatoid Factor IgM-Mouse ELISA Kit
小鼠免疫球蛋白M（IgM)

类风湿因子 ELISA试剂盒

 15.6～1000 mU/mL 100 μL

※ 本页面产品仅供研究用，研究以外不可使用。


参考文献

◆LBIS® 小鼠免疫球蛋白E（IgE） ELISA试剂盒相关参考文献


 1.

Inhibitory Effect of Carnosol on Phthalic Anhydride-Induced Atopic Dermatitis via Inhibition of STAT3. Lee DY, Hwang CJ, Choi JY, Park MH, Song MJ, Oh KW, Son DJ, Lee SH, Han SB, Hong JT. Biomol Ther (Seoul). 2017 Sep 1;25(5):535-544.


 2.

Effects of aqueous extract from red Liriope platyphylla on phthalic-anhydride-induced atopic dermatitis in Interleukin-4/Luciferase/Consensus non-coding sequence-1 transgenic mice evaluated in terms of luciferase signal and general phenotype biomarkers. Kwak MH, Kim JE, Go J, Son HJ, Lee HS, Hong JT, Hwang DY. Journal of Traditional Chinese Medicine, Vol.37, Issue 4, Aug 2017, p475-485

 3.

Oral administration of freeze-dried powders of honey bee larvae inhibits the development of atopic dermatitis-like skin lesions in NC/Nga mice. Kageyama M, Xing G, Li K, Zhang Z, Sugiyama A. Personalized Medicine Universe, Vol. 6, July 2017, Pages 22-27.

 4.

Differential Nitric Oxide-Derived Protein Modifications of Flavin-Containing Monooxygenases in Type 1 Allergic Mice. Tanino T, Bando T, Komada A, Nojiri Y, Okada Y, Ueda Y, and Sakurai E. Drug Metabolism and Disposition  July 31, 2017,  dmd.117.076570;

 5.

Simultaneously increased expression of glucocorticoid‑induced tumor necrosis factor receptor and its ligand contributes to increased interleukin‑5/13‑producing group 2 innate lymphocytes in murine asthma. Zhang M, Wan J, Xu Y, Zhang D, Peng J, Qi C, Guo Q, Xia S, Su Z, Wang S, Xu H. Mol Med Rep. 2017 Jun;15(6):4291-4299.

 6.

Transgenic rat model of childhood-onset dermatitis by overexpressing telomerase reverse transcriptase (TERT). Kaneko R, Sato A, Hamada S, Yagi T, Ohsawa I, Ohtsuki M, Kobayashi E, Hirabayashi M, Murakami T. Transgenic Res. 2016 Aug;25(4):413-24.

 7.

Effects of sublingual immunotherapy in a murine asthma model sensitized by intranasal administration of house dust mite extracts. Shima K, Koya T, Tsukioka K, Sakagami T, Hasegawa T, Fukano C, Ohashi-Doi K, Watanabe S, Suzuki E, Kikuchi T. Allergol Int. 2016 Jul 8.

 8.

Therapeutic effect of tyndallized Lactobacillus rhamnosus IDCC 3201 on atopic dermatitis mediated by down-regulation of immunoglobulin E in NC/Nga mice. Lee SH, Yoon JM, Kim YH, Jeong DG, Park S, Kang DJ. Microbiol Immunol. 2016 Jul;60(7):468-76.

 9.

Gain-switched 311-nm Ti:Sapphire laser might be a potential treatment modality for atopic dermatitis.. Choi SY, Oh CT, Kwon TR, Kwon HJ, Choi EJ, Jang YJ, Kim HS, Chu H, Mun SK, Kim MN, Kim BJ. Lasers Med Sci. 2016 Jul 9.

10.

Perilla leaf extract prevents atopic dermatitis induced by an extract of Dermatophagoides farinae in NC/Nga mice. Komatsu KI, Takanari J, Maeda T, Kitadate K, Sato T, Mihara Y, Uehara K, Wakame K. Asian Pac J Allergy Immunol. 2016 Mar 20.

11.

Functional polysaccharides from Grifola frondosa aqueous extract inhibit atopic dermatitis-like skin lesions in NC/Nga mice. Park HS, Hwang YH, Kim MK, Hong GE, Lee HJ, Nagappan A, Yumnam S, Kim EH, Heo JD, Lee SJ, Won CK, Kim GS. Biosci Biotechnol Biochem. Vol.79(1), p147-54, 2015.

12.

Inhibitory effect of 5,6-dihydroergosteol-glucoside on atopic dermatitis-like skin lesions via suppression of NF-κB and STAT activation. Jung M, Lee TH, Oh HJ, Kim H, Son Y, Lee EH, Kim J. J Dermatol Sci. 2015

13.

Immunopotentiator from Pantoea agglomerans Prevents Atopic Dermatitis Induced by Dermatophagoides farinae Extract in NC/Nga Mouse. Wakame K, Komatsu K, Inagawa H, Nishizawa T. Anticancer Res. Vol.35(8), p4501-8, Aug 2015.

14.

Solanum tuberosum L. cv Jayoung Epidermis Extract Inhibits Mite Antigen-Induced Atopic Dermatitis in NC/Nga Mice by Regulating the Th1/Th2 Balance and Expression of Filaggrin. Yang G, Cheon SY, Chung KS, Lee SJ, Hong CH, Lee KT, Jang DS, Jeong JC, Kwon OK, Nam JH, An HJ. J Med Food. 2015 Jun 23.

15.

Gamisasangja-tang suppresses pruritus and atopic skin inflammation in the NC/Nga murine model of atopic dermatitis. Park BK, Park YC, Jung IC, Kim SH, Choi JJ, Do M, Kim SY, Jin M. J Ethnopharmacol. 2015 Feb 23.

16.

Bacillus subtilis KCTC 11782BP-produced alginate oligosaccharide effectively suppresses asthma via T-helper cell type 2-related cytokines. Bang MA, Seo JH, Seo JW, Jo GH, Jung SK, Yu R, Park DH, Park SJ. PLoS One. 2015 Feb 6;10(2):e0117524.

17.

Effect of the hand antiseptic agents benzalkonium chloride, povidone-iodine, ethanol, and chlorhexidine gluconate on atopic dermatitis in NC/Nga mice. Sadakane K, Ichinose T. Int J Med Sci. Vol.5;12(2), p116-25, Jan 2015.

18.

Effects of oral administration of di-(2-ethylhexyl) and diisononyl phthalates on atopic dermatitis in NC/Nga mice. Sadakane K., Ichinose T., Takano H., Yanagisawa R. and Koike E. Immunopharmacology and Immunotoxicology, Vol.36(1), p61-69, Feb 2014.

19.

Oral Administration of SSC201, a Medicinal Herbal Formula, Suppresses Atopic Dermatitis-Like Skin Lesions. Kyung P., Chun P., Chul J., Hyung K., Eun C., Sunyoung P., June C. and Mirim J. Journal of Medicinal Food, 2014.

20.

Effect of Allergen Sensitization on External Root Resorption. Murata N., Ioi H., Ouchi M., Takao T., Oida H., Aijima R., Yamaza T., Kido MA. JDR, Vol.92(7), p641-647, Jul 2013.

21.

Quantitative evaluation of therapeutic effect of Liriope platyphylla on phthalic anhydride-induced atopic dermatitis in IL-4/Luc/CNS-1 Tg mice. M.H.Kwak, J.E.Kim, I.S.Hwang, Y.J.Lee, B.S.An, J.T.Hong, S.H.Lee, D.Y.Hwang. Journal of Ethnopharmacology, Vol.148(3), p880-889, Jul 2013.

22.

Lack of both α2-antiplasmin and plasminogen activator inhibitor type-1 induces high IgE production. K.Okada, S.Ueshima, N.Kawao, M.Yano, Y.Tamura, M.Tanaka, A.Sakamoto, M.Hatano, M.Arima, S.Miyata, N.Nagai, T.Tokuhisa, O.Matsuo. Life Sciences, Vol.93(2-3), p89-95, Jul 2013.

23.

Therapeutic effects of fermented soycrud on phenotypes of atopic dermatitis induced by phthalic anhydride. Sung J-E., Kwak M-H., Kim J-E., Lee Y-J., Kim R-U., Kim E-A., Lee G-Y., Kim D-S. and Hwang D-Y. Lab Anim Res, Vol.29(2), p103-112, Jun 2013.

24.

Water-soluble chitosan and herbal honey compound alleviates atopic dermatitis-like lesions in NC/Nga mice. C.Choi, W.-S.Kim, Y.H.Park, S.-C.Park, M.-K.Jang, J.-W.Nah. Journal of Industrial and Engineering Chemistry, May 2013.

25.

Interleukin-17/T-helper 17 cells in an atopic dermatitis mouse model aggravate orthodontic root resorption in dental pulp. M.Shimizu, M.Yamaguchi, S.Fujita, T.Utsunomiya, H.Yamamoto, K.Kasai. European Journal of Oral Sciences, Vol.121(2), p101-110, Apr 2013.

26.

Water-soluble chitosan and herbal honey compound alleviates atopic dermatitis-like lesions in NC/Nga mice. Choi C., Kim W-S., Park YH., Park S-C., Jang M-K., Nah J-W. Journal of Industrial and Engineering Chemistry, Vol.20(2), p499-504, Mar 2014.

27.

Indirubin, a purple 3,2- bisindole, inhibited allergic contact dermatitis via regulating T helper (Th)-mediated immune system in DNCB-induced model. Kim M H, Choi Y Y, Yang G, Cho I-H, Nam D, Yang W M. Journal of Ethnopharmacology, Vol.145(1), p214-219, Jan 2013.

28.

Effects of Catalpa ovata stem bark on atopic dermatitis-like skin lesions in NC/Nga mice. Yang G, Choi C-H, Lee K, Lee M, Ham I, Choi H-Y. Journal of Ethnopharmacology, Vol.145(2), p416-423, Jan 2013.

29.

Interleukin 33 Is Induced by Tumor Necrosis Factor α and Interferon γ in Keratinocytes and Contributes to Allergic Contact Dermatitis. Taniguchi K., Yamamoto S., Hitomi E., Inada Y., Suyama Y., Sugioka T., Hamasaki Y. J Investig Allergol Clin Immunol, Vol.23(6), p428-434, 2013.

30.

Organic Chemicals in Diesel Exhaust Particles Enhance Picryl Chloride-Induced Atopic Dermatitis in NC/Nga Mice. Sadakane K., Ichinose T., Takano H., Yanagisawa R., Inoue K., Kawazato H., Yasuda A., Hayakawa K. Int Arch Allergy Immunol, Vol.162(1), p7-15, 2013.

31.

The effects of (±)-Praeruptorin A on airway inflammation, remodeling and transforming growth factor-β1/Smad signaling pathway in a murine model of allergic asthma. Xiong Y, Wang J, Wu F, Li J, Kong L. International Immunopharmacology, Vol.14(4), p392-400, Dec 2012.

32.

Inhibitory effects of Drynaria fortunei extract on house dust mite antigen-induced atopic dermatitis in NC/Nga mice. Sung Y-Y, Kim D-S, Yang W-K, Nho K J, Seo H S, Kim Y S, Kim H K.. Journal of Ethnopharmacology, Vol.144(1), p94-100, Oct 2012.

33.

Topical application of an ethanol extract prepared from Illicium verum suppresses atopic dermatitis in NC/Nga mice. Sung Y-Y, Yang W-K, Lee A Y, Kim D-S, Nho K J, Kim Y S, Kim H K.. Journal of Ethnopharmacology, Vol.144(1), p151-159, Oct 2012.

34.

Anti-allergic effect of lactic acid bacteria isolated from seed mash used for brewing sake is not dependent on the total IgE levels. Masuda Y, Takahashi T, Yoshida K, Nishitani Y, Mizuno M, Mizoguchi H. Journal of Bioscience and Bioengineering, Vol.114(3), p292-296, Sep 2012.

35.

Advantages of histamine H4 receptor antagonist usage with H1 receptor antagonist for the treatment of murine allergic contact dermatitis. Matsushita A, Seike M, Okawa H, Kadawaki Y, Ohtsu H. Experimental Dermatology, Vol.21(9), p714-715, Sep 2012.

36.

Platycodon grandiflorus alleviates DNCB-induced atopy-like dermatitis in NC/Nga mice. Park S-J, Lee H-A, Kim J W, Lee B-S and Kim E-J. Indian J Pharmacol. Vol.44(4), p469-474, Jul-Aug 2012.

37.

Effect of Chrysanthemi borealis flos on atopic dermatitis induced by 1-chloro 2,4-dinitrobenzene in NC/Nga mouse. Yang G, Lee K, An D-G, Lee M-H, Ham I-H, Choi H-Y. Immunopharmacology and Immunotoxicology, Vol.34(3), p413-418, Jun 2012.

38.

Effects of (±)-praeruptorin A on airway inflammation, airway hyperresponsiveness and NF-κB signaling pathway in a mouse model of allergic airway disease. Xiong Y, Wang J, Wu F, Li J, Zhou L, Kong L. European Journal of Pharmacology, Vol.683(1-3), p316-324, May 2012.

39.

Anti-allergic effect of lactic acid bacteria isolated from seed mash used for brewing sake is not dependent on the total IgE levels. Y. Masuda., T. Takahashi., K. Yoshida., Y. Nishitani., M. Mizuno., H. Mizoguchi. Journal of Bioscience and Bioengineering, 2012.

40.

The Protective and Recovery Effects of Fish Oil Supplementation on Cedar Pollen-Induced Allergic Reactions in Mice. Hirao A, Furutani N, Nagahama H, Itokawa M, Shibata S. Food and Nutrition Sciences, Vol.3, p40-47, 2012.

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Th17-cells in atopic dermatitis stimulate orthodontic root resorption. Yamada K, Yamaguchi M, Asano M, Fujita S, Kobayashi R, Kasai K. Oral Diseases, 2012.

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Mixture of Polyphenols and Anthocyanins from Vaccinium uliginosum L. Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice. Kim M J and Choung S-Y. Evidence-Based Complementary and Alternative Medicine, Vol.2012 (2012)

43.

Single systemic administration of Ag85B of mycobacteria DNA inhibits allergic airway inflammation in a mouse model of asthma. Karamatsu K, Matsuo K, Inada H, Tsujimura Y, Shiogama Y, Matsubara A, Kawano M and Yasutomi Y. J Asthma Allergy, Vol.5, p71-79, 2012.

44.

Inhibitory Effect of Nelumbo nucifera (Gaertn.) on the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice. R. Karki., M.-A Jung., K.-J. Kim., and D.-W Kim. Evidence-Based Complementary and Alternative Medicine, Vol. 2012 (2012), Article ID 153568, 7 pages

45.

Lack of Transient Receptor Potential Vanilloid-1 Enhances Th2-Biased Immune Response of the Airways in Mice Receiving Intranasal, but Not Intraperitoneal, Sensitization. T. Mori., K. Saito., Y. Ohki., H. Arakawa., M. Tominaga., K. Tokuyama. Int Arch Allergy Immunol 2011, Vol.156, p305-312.

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Improvement of the Antiinflammatory and Antiallergic Activity of Bidens pilosa L. var. radiata SCHERFF Treated with Enzyme (Cellulosine). Horiuchi, M. and Seyama, Y. Journal of Health Science, Vol. 54(2008), No.3, p294-301.

47.

Suppression of spontaneous dermatitis in NC/Nga murine model by PG102 isolated from Actinidia arguta. Park, E.J., Park, K.C., Eo, H, Seo, J., Son, M., Kim, K.H. et al. J Investigative Dermatology, Vol.127, p1154-1160, 2007.

48.

Sopungyangjae-Tang inhibits development of dermatitis in Nc/Nga mice. Pokharel, Y.R., Lim, S.C., Kim, S.C., Heo, T.H., Choi, H.K. and Kang, K.W. Evidence-based Complementary and Alternative Medicine nem015, 1-8, March 12, 2007.

49.

Inhibitory effect of DA-9201, an extract of Oryza sativa L., on airway inflammation and bronchial hyperresponsiveness in mouse asthma model. Lee, S.H., Sohm, Y.S., Kang, K.K., Kwon, J.W. and Yoo, M. Biol. Pharm. Bull. Vol.29(6), p1148-1153, 2006.

50.

A new symbiotic, Lactobacillus casei subsp. casei together with dextran, reduces murine and human allergic reaction. Ogawa, T., Hashikawa, S., Asai, Y., Sakamoto, H., Yasuda, K., and Makimura, Y. FEMS Immunology and Medical Microbiology, Vol.46, p400-409, 2006.

51.

Effects of alpha tocopherol and probucol supplements on allergen-induced airway inflammation and hyperresponsiveness in a mouse model of allergic asthma. Okamoto, N., Murata, T., Tamai, H., Tanaka, H., and Nagai, H. Int. Allergy Immunol. Vol.141, p172-180, 2006.

52.

Control of IgE and selective TH1 and TH2 cytokines by PG102 isolated from Actinidia arguta. Parkm E.J., Kim, B., Eo, H., Park, K, Kim, Y., Lee, H.J. and Son, M. J Allergy and Clinical Immunol, p1151-1167, 2005.

53.

Oral immunotherapy against a pollen allergy using a seed-based peptide vaccine. Takagi, H., Saito, S., Yang, L., Nagasawa, S., Nishizawa, N., and Takaiwa, F. Plant Biotechnology Journal, Vol.3, p521-533, 2005.

54.

Activated protein C inhibits bronchial hyperresponsiveness and Th2 cytokine expression in mice. Hisamichi Yuda, Yukihiko Adachi, Osamu Taguchi, Esteban C. Gabazza, Osamu Hataji, Hajime Fujimoto, Shigenori Tamaki, Kimiaki Nishikubo, Kenji Fukudome, Corina N. D’Alessandro-Gabazza, Junko Maruyama, Masahiko Izumizaki, Michiko Iwase, Ikuo Homma, Ryo Inoue, Haruhiko Kamada, Tatsuya Hayashi, Michael Kasper, Bart N. Lambrecht, Peter J. Barnes, and Koji Suzuki. Blood, Vol.103, No.6, p2196-2204, 2004.

55.

Inadequate induction of suppressor of cytokine signaling-1 causes systemic autoimmune diseases. Fujimoto, M., Tsutsui, H., Xinshou, O., Tokumoto, M., Watanabe, D., Shima, Y., Yoshimoto, T., Hirakata, H., Kawase, I., Nakanishi, K., Kishimoto, T., and Naka, T. International Immunology Vol.16, p303-314, 2004.

56.

Association of cathepsin E deficiency with development of atopic dermatitis. Tsukuba, T., Okamoto, K., Okamoto, Y., Yanagawa, M. J Biochemistry, Vol.134, p893-902, 2003.

57.

Mast-cell-dependent histamine release after praziquantel treatment of Schistosoma japonicum infection: implications for chemotherapy-related adverse effects. Jun Matsumoto and Hajime Matsuda. Parasitology Research, Vol.88, p888 – 893, 2002.

◆LBIS® 大鼠免疫球蛋白E（IgE） ELISA试剂盒相关参考文献


1.

Transgenic rat model of childhood-onset dermatitis by overexpressing telomerase reverse transcriptase (TERT). Ryosuke Kaneko, Atsuko Sato, Shun Hamada, Takeshi Yagi, Ichiro Ohsawa, Mamitaro Ohtsuki, Eiji Kobayashi, Masumi Hirabayashi, Takashi Murakami. Transgenic Research, pp 1-12, First online: 17 February 2016

2.

Oral administration of drugs with hypersensitivity potential induces germinal center hyperplasia in secondary lymphoid organ/tissue in Brown Norway rats, and this histological lesion is a promising candidate as a predictive biomarker for drug hypersensitivity occurrence in humans. A.Tamura, I.Miyawaki, T.Yamada, J.Kimura, H.Funabashi. Toxicology and Applied Pharmacology, Vol.271(1), p30-40, Aug 2013.

3.

Enhancement of the pulmonary allergic granulocyte recruitment in rats exposed to DMTI-Ⅱ,a Kunitz-type inhibitor isolated from Dimorphandra mollis seeds. Glaucia,C,M.,Karina,F,R.,Dalize,M,S.,Andre,A,S.,Ivani,A,de S.,Maria,L R,M.,Edson,A.. International Immunopharmacology, Vol.11(6),  p740-747, Jun 2011.

4.

Altered cytokine expression in mesenteric lymph nodes in a rat strain (Matsumoto Eosinophilic Shinshyu) that spontaneously develops hypereosinophilia. Muto, S., Monnai, M., Okuhara, Y., Murakami, M., Kuroda, J., Ono, T., and Matsumoto, K.. Immunology, Vol.116, p373-380, 2005.

◆LBIS® 小鼠免疫球蛋白G（IgG）类风湿因子ELISA试剂盒相关参考文献


 1.

Dysbiosis Contributes to Arthritis Development via Activation of Autoreactive T Cells in the Intestine. Maeda Y, Kurakawa T, Umemoto E, Motooka D, Ito Y, Gotoh K, Hirota K, Matsushita M, Furuta Y, Narazaki M, Sakaguchi N, Kayama H, Nakamura S, Iida T, Saeki Y, Kumanogoh A, Sakaguchi S, Takeda K. Arthritis Rheumatol. 2016 Nov;68(11):2646-2661.

 2.

C9orf72 ablation causes immune dysregulation characterized by leukocyte expansion, autoantibody production, and glomerulonephropathy in mice. Atanasio A, Decman V, White D, Ramos M, Ikiz B, Lee HC, Siao CJ, Brydges S, LaRosa E, Bai Y, Fury W, Burfeind P, Zamfirova R, Warshaw G, Orengo J, Oyejide A, Fralish M, Auerbach W, Poueymirou W, Freudenberg J, Gong G, Zambrowicz B, Valenzuela D, Yancopoulos G, Murphy A, Thurston G, Lai KM. Sci Rep. 2016 Mar 16

 3.

Reciprocal effects of Schistosoma mansoni infection on spontaneous autoimmune arthritis in IL-1 receptor antagonist-deficient mice. Osada Y, Yamada S, Nakae S, Sudo K, Kanazawa T. Parasitol Int. Vol.64(1), p13-17. Feb 2015.

 4.

The development of mixed cryoglobulinemia in Capillaria hepatica-infected mice is associated with the capillaria antigen-induced selective proliferation of splenic B-1a cells in response to interleukin-5 stimulation. Aihara N, Kamiie J, Yamada M, Shirota K. Am J Pathol. Vol.185(1), p172-84, Jan 2015.

 5.

Mechanism of the 24-hour rhythm of tumor necrosis factor-alpha formed by onset of rheumatoid arthritis. Yoshimatsu H., Okazaki F., Ieiri I., To H. Chronobiology International, 2014.

 6.

Deficient Leptin Signaling Ameliorates Systemic Lupus Erythematosus Lesions in MRL/Mp-Faslpr Mice. Fujita Y., Fujii T., Mimori T., Sato T., Nakamura T., Iwao H., Nakajima A., Miki M., Sakai T., Kawanami T., Tanaka M., Masaki Y., Fukushima T., Okazaki T. and Umehara H. The Journal of Immunology, Vol.192(3), p979-984, Feb 2014.

 7.

Phenotype conversion from rheumatoid arthritis to systemic lupus erythematosus by introduction of Yaa mutation into FcγRIIB-deficient C57BL/6 mice. Kawano S, Lin Q, Amano H, Kaneko T, Nishikawa K, Tsurui H, Tada N, Nishimura H, Takai T, Shirai T, Takasaki Y, Hirose S. European Journal of Immunology, Vol.43(3), p770-778, Mar 2013.

 8.

IFN-γ-Producing Effector CD8 T Lymphocytes Cause Immune Glomerular Injury by Recognizing Antigen Presented as Immune Complex on Target Tissue. Tsumiyama K., Hashiramoto A., Takimoto M., Tsuji-Kawahara S., Miyazawa M. and Shiozawa S. The Journal of ImmunologyJuly, Vol.191(1), p91-96, 2013.

 9.

Increased expression of soluble form of vascular cell adhesion molecule-1 aggravates autoimmune arthritis in MRL-Fasipr mice. Oishi, H., Mizuki, S., Terada, M., Kudo, M., Araki, K., Araki, M., Nose, M., and Takahashi, S. Pathology International, Vol.57, p734-740, 2007.

10.

Electro-acupuncture at acupoint ST36 reduces inflammation and regulates immune activity in collagen-induced arthritic mice. Yim, Y.Y., Lee, H., Hong, K.E., Kim, Y.I., Lee, B.R., Son, C.G., and Kim, J.E. Evidence-based Complementary and Alternative Medicine 4:p51-57, 2007.

11.

Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment. Kumanogoh, A., Shikina, T., Watanabe, C., Takegahara, N., Suzuki, K., Yamamoto, M., Takamatsu, H., Prasad, D.V.R., Mizui, M., Toyohuku, T., Tamaru, M., Watanabe, D., Parnes, J.R., and Kikutani, H. International Immuno, Vol.17, p1277-1282, 2005.

12.

The point mutation of tyrosine 759 of the IL-6 family cytokine receptor gp130 synergizes with HTLV-1 pX in promoting rheumatoid arthritis-like arthritis. Ishihara, K., Sawa, S., Ikushima, H., Hirota, S., Atsumi, T., Kamimura, D., Park, S.J., Murakami, M., Kitanuma, Y., Iwakura, Y., and Hirano, T. International Immunol. Vol.16, p455-465, 2004.

13.

Chemical induction of HO-1 suppresses lupus nephritis by reducing local iNOS expression and synthesis of anti-dsDNA antibody. Takeda, Y., Takeno, M., Iwasaki, M., Kobayashi, H., Kirino, Y., Ueda, A., Nagahama, K., Aoki, I., and Ishigatsubo, Y. Clin Exp Immunol. Vol.138, p237-244, 2004.

14.

A Point Mutation of Tyr-759 in Interleukin 6 Family Cytokine Receptor Subunit gp130 Causes Autoimmune Arthritis. T. Atsumi., K. Ishihara., D. Kamimura., H. Ikushima., T. Ohtani., S. Hirota., H. Kobayashi., S. Park., Y. Saeki., Y. Kitamura. and T. Hirano. J Exp Med, 196, Number 7, p979-990, 2002.

15.

A novel assay kits for autoantibodies rate on spontaneous autoimmune model mice. Kikukawa, T, Kojima, M., and Abe, C. Jap J Inflammation, Vol.20, p697-701, 2000.

◆LBIS® 小鼠免疫球蛋白M（IgM）类风湿因子ELISA试剂盒相关参考文献


 1.

C9orf72 ablation causes immune dysregulation characterized by leukocyte expansion, autoantibody production, and glomerulonephropathy in mice.
Atanasio A, Decman V, White D, Ramos M, Ikiz B, Lee HC, Siao CJ, Brydges S, LaRosa E, Bai Y, Fury W, Burfeind P, Zamfirova R, Warshaw G, Orengo J, Oyejide A, Fralish M, Auerbach W, Poueymirou W, Freudenberg J, Gong G, Zambrowicz B, Valenzuela D, Yancopoulos G, Murphy A, Thurston G, Lai KM.

Sci Rep. 2016 Mar 16

 2.

Production of Autoantibodies by Murine B-1a Cells Stimulated with Helicobacter pylori Urease through Toll-Like Receptor 2 Signaling
Kobayashi F, Watanabe E, Nakagawa Y, Yamanishi S, Norose Y, Fukunaga Y and Takahashi H.

Infect. Immun, Vol.79(12), p4791-4801, Dec 2011.

 3.

Lack of B and T lymphocyte attenuator exacerbates autoimmune disorders and induces Fas-independent liver injury in MRL-lpr/lpr mice
Y. Oya., N. Watanabe. Y. Kobayashi., T. Owada., M. Oki., K. Ikeda. A. Suto., S. Kagami., K. Hirose., T. Kishimoto., and H. Nakajima.

Int. Immunol. Vol.23(5), p335-344, 2011.

 4.

An experimental type 2 mixed cryoglobulinemia with renal glomerulopathy in ICR mice triggered by Capillaria hepatica infection
N. Aihara., J. K. Chambers., K. Hosono., J. Kamiie., and K. Shirota.

Histol Histopathol, Vol.26, p1519-1529, 2011.

 5.

Point mutation of tyrosine 759 of the IL-6 family cytokine receptor,gp130,augments collagen-induced arthritis in DBA/IJ mice
Tsuji,F.,Yoshimi,M.,Katsuta,O.,Takai,M.,Ishihara,K.,Aono,H.

BMC Musculoskeletal Disorders ,10:23, 2009.

 6.

Implications for induction of autoimmunity via activation of B-1 cells by Helicobacter pylori urease.
Yamasnishi, S., Iizumi, T., Watanabe, E., Shimizu, M., Kamiya, S., Nagata, K., Kumagai, Y., Fukunaga, Y., and Takahashi, H.

Infection and Immunity, Vol.74, p248-256, 2006.

 7.

Cytokine production profile of splenocytes derived from zymosan A-treated SKG mice developing arthritis.
Kobayashi, K., Suda, T., Nan-ya, K., Sakaguchi, N., Sakaguchi, S., and Miki, I.

Inflammation Research, Vol.55, p335-341, 2006.

 8.

Chronic polyarthritis caused by mammalian DNA that escapes from degradation in macrophages.
Kawane, K., Ohtani, M., Miwa, K., Kizawa, T., Kanbara, Y., Yoshioka, Y., and Yoshikawa,H.

Nature, Vol.443, p998-1002, 2006.

 9.

Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment.
Kumanogoh, A., Shikina, T., Watanabe, C., Takegahara, N., Suzuki, K., Yamamoto, M., Takamatsu, H., Prasad, D.V.R., Mizui, M., Toyohuku, T., Tamaru, M., Watanabe, D., Parnes, J.R., and Kikutani, H.

International Immuno, Vol.17, p1277-1282, 2005.

10.

A Point Mutation of Tyr-759 in Interleukin 6 Family Cytokine Receptor Subunit gp130 Causes Autoimmune Arthritis
T. Atsumi., K. Ishihara., D. Kamimura., H. Ikushima., T. Ohtani., S. Hirota., H. Kobayashi., S. Park., Y. Saeki., Y. Kitamura. and T. Hirano

J Exp Med, 196, Number 7, p979-990, 2002.

11.

A novel assay kits for autoantibodies rate on spontaneous autoimmune model mice.
Kikukawa, T, Kojima, M., and Abe, C.

Jap J Inflammation, Vol.20, p697-701, 2000.

Biodesign公司介绍

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Biodesign公司介绍

简要描述：Biodesign公司提供多种疾病相关抗原、抗体、工业诊断用大包装免疫原料。

详细介绍

产品咨询

公司：www.biodesign.com

Biodesign公司提供多种疾病相关抗原、抗体、工业诊断用大包装免疫原料。

货号 产品名称 规格
Z87304M  MAb to Human IgE 0.5mg 
Z87210P  MAb to Human IgE 0.5mg 
Z86411M  MAb to IgE Fc 1mg 
Z86410M  MAb to IgE 1mg 
Z86301M  MAb to Hu IgM 1mg 
Z86238M  MAb to IgG （Fc） 1mg 
Z86215M  MAb to Human IgM 1mg 
Z86213P  MAb to IgG Fc 1mg 
Z86213M  MAb to IgG （Fc） 1mg 
Z86210M  MAb to IgA1 1mg 
Z86201P  MAb to IgA 1mg 
Z86201M  MAb to IgA 1mg 
Z86200M  MAb to IgA 1mg 
Z86140M  MAb to IgG4 （Fc） 1mg 
Z86130M  MAb to Hu IgG3 1mg 
Z86114P  MAb to Human IgM （Heavy Chains） 1mg 
Z86114M  MAb to Hu IgM （Heavy Chains） 1mg 
Z86113M  MAb to Hu IgM 1mg 
Z86110M  MAb to Hu IgM （Heavy Chains） 1mg 
Z86108M  MAb to Free Kappa Light Chains 1mg 
Z86107M  MAb to Free Lambda Light Chains 1mg 
Z86106M  MAb to Lambda Light Chains 1mg 
Z86105M  MAb to Kappa Light Chains 1mg 
Z86104M  MAb to IgE （Fc） 1mg 
Z86102M  MAb to IgG1 （Fc） 1mg 
Z86101P  MAb to IgG Fc 1mg 
Z86101M  MAb to IgG （Fc） 1mg 
Z86020M  MAb to IgA 1mg 
Z86003M  MAb to IgA2 （Fc） 1mg 
Z86002M  MAb to IgG2 （Fc gamma-2） 1mg 
Z86001M  MAb to IgG2, hinge region 1mg 
Z6E20-32016  DDE Ovalbumin Conjugate 1mg 
Z6D63-2595  Theophylline, BSA Conjugated 10mg 
Z6D53-3405  Digoxin BSA Conjugate 25mg 
Z54101M  MAb to IgG （Fc） 0.5mg 
Z45904M  MAb to Human IgE 1mg 
Z45802M  MAb to Human IgA 1mg 
Z45190M  MAb to Human IgM 1mg 
Z45180M  MAb to Human IgE 1mg 
Z45175M  MAb to Human IgA 1mg 
Z42750M  MAb to Human IgA （Secretory） 0.5mg 
Z30117F  MAb to Hu IgM （Heavy Chains） 100 Tests 
Z30114F  MAb to Hu IgA （Heavy Chains） 100 Tests 
Z20872M  MAb to Hu IgG2 （Fc） 1mg 
Z20185M  MAb to Human IgE 1mg 
Z20154M  MAb to Hu IgG4 （Fc） 1mg 
Z20142M  MAb to Hu IgG （Heavy Chains） 1mg 
Z0B02-194  Protein A, Recombinant 1g 
Y82992B  THC Delta 8-BSA Ag Conjugate 1mg 
Y82932B  Methamphetamine-N-BSA Ag. Conjugate 1mg 
Y82611A  Phenobarbital BSA Conjugate 1mg 
Y82609A  Morphine BSA Conjugate 1mg 
Y82451P  Cotinine-4-HRP Conjugate 1ml 
Y82290P  Cotinine-3-HRP Conjugate 1ml 
Y82250B  Oxycodone BSA Conjugate 1mg 
Y82230P  Testosterone 1ml 
Y82135P  Methamphetamine-S-HRP Conjugate 1ml 
Y82125P  THC Delta 8-HRP Conjugate 1ml 
Y82115P  HRP Conj. to Benzoylecgonine 1ml 
Y49932B  Methamphetamine-BSA Conjugate 0.2mg 

abbiotec 250205说明书

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abbiotec 250205说明书

Human-IgE

无叠氮抗体

 

对照抗体

 

人源免疫球蛋白
货号：250205
规格：1毫克
$ 2,712.00
描述

该产品是纯化的人IgE，具有从杂交瘤细胞体外产生的κ轻链。正常患者的血浆IgE浓度较低。在出现严重过敏反应和寄生虫感染的患者中发现IgE水平升高。原始材料来自健康的捐赠者，使用美国FDA批准的测试方法对HIV，HCV和B型肝炎呈阴性。通过SDS-PAGE分析，人IgE的纯度> 98％。由于细胞系的起源，不会被其他同种型的抗体污染。通过Protein-L亲和色谱法除去胎牛血清中的污染物。该材料必须与所有人类材料一样具有潜在的传染性。该产品可用作定量测定和细胞致敏测定中的IgE对照。

 

同型
人IgE
应用领域
乙
Ë
世界银行
物种反应性
H
格式

 

每个小瓶在1 ml（1 mg / ml）PBS pH7.4中包含1 mg IgE。没有叠氮化纳。通过蛋白-L亲和层析纯化抗体。1 mg人IgE相当于380,000±50,000 IU / ml。
备用名
免疫球蛋白E; 免疫球蛋白

 

克隆性
HE1
抗原
这是一种非免疫杂交瘤。
应用须知
产品目录 250205号可以1-10 ug /孔包被，用于ELISA分析。具有κ轻链的纯化的人IgE是在体外从单克隆杂交瘤中产生的。原始材料来自健康的捐赠者，使用美国FDA批准的测试方法对HIV，HCV和B型肝炎呈阴性。通过SDS-PAGE分析，人IgE的纯度> 98％。内毒素水平<25 EU / mg。
贮存
储存在-20°C。最小化冻融循环。该材料必须与所有人类材料一样具有潜在的传染性。产品自发货之日起一年内保修。

 

columbiabiosciences D5-112-E说明书

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columbiabiosciences D5-112-E说明书

 

Goat anti-Mouse IgE: SureLight™ R-PE, 100ug

$207.00

 SKU: D5-112-E

Category: Anti-Mouse

 

描述

与SureLight™R-PE共轭的山羊抗小鼠IgE，100ug

抗体： 山羊多克隆抗小鼠IgE

同种型： IgE

用途： 流式细胞仪，基于细胞的测定，微阵列和微孔板应用

 

USE:

Flow Cytometry/FACS Protein Microarrays

ANTIBODY:

Anti-Mouse

SIZE:

100μg

DYE:

SureLight™ R-phycoerythrin

 

absoluteantibody抗杏仁过敏原详情

发表于由

absoluteantibody

 

抗体是免疫系统用来保护我们免受各种病原体侵害的强大工具。但是，抗体也可能具有有害作用，正如I型超敏反应所广泛证明的那样，尤其是我们通常所说的“过敏”。

无论是花粉，蜜蜂叮咬还是各种食物-过敏都很普遍，关于它们为何呈上升趋势的原因也有各种各样的理论。

为了迎合研究过敏症的研究人员，我们提供了一系列针对常见过敏原的抗体。这些抗体中的许多已显示与天然IgE竞争，IgE是介导过敏的主要抗体同种型。我们提供这些抗体作为人类IgE（以及针对IgE本身的抗体），用作校准物和阳性对照。

 

absoluteantibody抗杏仁过敏原[X151]

描述： 

杏仁反应原的重组单克隆抗体。使用具有来自杂交瘤X151的可变区（即特异性）的AbAb重组平台生产。

克隆名称：

X151

已发布的应用程序：

ELISA法

已发布物种反应性：

杏仁），李（扁桃）

 

  • Ab01111-1.1抗杏仁过敏原[X151]
  • 杏仁），李（扁桃）
  • 小鼠IgG1
  •  
  • 净化的
  • 在4-5周内发货
  •  
  • Ab01111-20.0抗杏仁过敏原[X151]
  • 杏仁），李（扁桃）
  • 小鼠IgE
  •  
  • 净化的
  • 在4-5周内发货
  •  
  • Ab01111-10.0抗杏仁过敏原[X151]
  • 杏仁），李（扁桃）
  • 人IgG1
  •  
  • 净化的
  • 在4-5周内发货
  •  
  • Ab01111-10.3抗杏仁过敏原[X151]
  • 杏仁），李（扁桃）
  • 人IgG1
  • Fc Silent™
  • 净化的
  • 在4-5周内发货
  •  
  • Ab01111-14.0抗杏仁过敏原[X151]
  • 杏仁），李（扁桃）
  • 人IgE
  •  
  • 净化的
  • 在4-5周内发货
  •  
  • Ab01111-13.12抗杏仁过敏原[X151]
  • 杏仁），李（扁桃）
  • 人IgG4-S228P
  •  
  • 净化的
  • 在4-5周内发货
  •  
  • Ab01111-23.0抗杏仁过敏原[X151]
  • 杏仁），李（扁桃）
  • 兔IgG
  •  
  • 净化的
  • 在4-5周内发货

 

 

 

免疫原：通过用纯化的杏仁过敏原免疫小鼠来产生该抗体。

特异性：该抗体识别主要的杏仁过敏原。

应用说明：该抗体的特异性已通过ELISA测试确认。其增强的IgE和人IgG4版本可在体外和体内过敏模型中用作对照。后，该抗体的经过工程改造的IgG4版本可用于过敏治疗的研究中，该研究使用IgG阻断过敏生物系统中的游离过敏原并防止过敏原与IgE结合（Orengo等人，2018）。

抗体*发表于： PMID：

 

 

NBS-C BioScience公司介绍

发表于由

 

NBS-C BioScience公司介绍

为什么要使用NBS-C生物科学的人类IgE？

 

·免疫亲和层析纯化IgE特异性单克隆抗体。用分析凝胶渗透色谱法（SuSOSE 12／HR）确认高纯度

 

·通过监测人体高亲和力IgE受体的实时结合动力学（表面等离子体共振技术；Biacore™）对每一批进行功能测试。

 

·可作为参考标准，根据世卫组织IgE参考（世卫组织，第二IRP，75/502）校准，用于定量IgE测量，与外部（临床）研究数据具有*的可比性。

 

·可根据用户要求提供不同配方，如缓冲器、防腐剂、标签、批量订单等。

 

·20多年来，许多实验室的研究人员在*范围内使用，在同行评审期刊中引用。

eaglebio R111说明书

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eaglebio R111说明书

 

Omalizumab IgE ELISA Assay

 SKU: R111

 

奥马珠单抗IgE ELISA测定

$ 995.00

Omalizumab IgE ELISA测定法是在人血清样品中可用治疗性抗体Omalizumab影响下定量测定游离IgE和可用Omalizumab的测试试剂盒。它由一个手动的非自动化试剂盒组成，用于测定7个样品。Omalizumab Free IgE ELISA分析试剂盒仅供研究使用，不得用于诊断程序。

 

 

奥马珠单抗IgE ELISA测定

Omalizumab IgE ELISA分析仅用于研究

大小：1×96
孔灵敏度：1 IU / ml
动态范围：1 – 2000 IU / ml（样品中不含Omalizumab），1-500 IU / ml（存在Omalizumab）。Omalizumab的浓度可以在0.4-80 µg / ml之间确定
孵育时间：隔夜
样品类型：血清
样品量：30 µL
替代名称：Omalizumab Free IgE ELISA，Xolair

包括的对照
* IgE的浓度是根据WHO参考试剂，NIBSC代码进行校准的：75/502 
** 1 IU对应于2.4 ng IgE

分析背景

过敏性哮喘是一种广泛的疾病，由引起过敏的物质（过敏原）引发或加重。E型免疫球蛋白（IgE）是在生物体中形成的，与变应原反应，使变应性的递质（如组胺，白三烯和缓激肽）从肥大细胞中开始分布。在立即发生的I型反应中，吸入过敏原直接导致气道狭窄。

在特别严重的过敏性支气管哮喘病例中，使用治疗性抗体Omalizumab（针对IgE的重组人源化抗体），负责触发过敏反应的IgE通过结合被中和。奥马珠单抗是一种抗抗体，可防止级联过敏反应并减少IgE与肥大细胞的结合。奥马珠单抗的治疗目标是降低生物体中的IgE水平。

回收ELISA Omalizumab游离IgE ELISA分析试剂盒可在使用Omalizumab的同时确定游离IgE和可用的治疗性抗体（仅供研究使用）。

相关产品

Omalizumab（Xolair）ELISA试剂盒
抗Omalizumab（Xolair）ELISA测定
基于Omalizumab mAb的ELISA