Hampton 蛋白结晶优化试剂 Optimization Screens

Hampton 蛋白结晶优化试剂 Optimization Screens

1、Solubility & Stability Screen  蛋白特异性试剂优化溶解性和稳定性
Protein specific agents for optimization of solubility & stability  
2、Solubility & Stability Screen 2   离子缓冲液
Buffer, pH & ionic strength screen for optimization of solubility & stability
3、Slice pH    PH缓冲液
pH & buffer screen for optimization of crystals or sample solubility & stability
4、Additive Screen  96孔板添加剂试剂盒
96 compound screen for optimization of crystal size & quality
5、Silver Bullets • Silver Bullets Bio
Small molecule additive screen for optimization of crystal size & quality
6、Detergent Screen
Detergent screen for optimization of crystals or sample solubility & stability
7、Ionic Liquid Screen
Ionic liquid additive screen for optimization of crystal size & quality
8、StockOptions Buffer Kits
Titrated buffer sets for optimization of crystals or sample solubility & stability
9、StockOptions Polymer
n of crystal size & quality
10、StockOptions Salt
Salt reagent kit for optimization of crystal size & qualit
11、Polymer reagent kit for optimizatioy
Proti-Ace
In situ proteolysis kit protein for crystallization screening & optimization
12、Reductive Alkylation
Reductive alkylation kit for crystallization screening & optimization
13、Silica Hydrogel Kit
pH neutral silica hydrogel matrix crystallization
14、Low Melting Agarose
Agarose gel matrix crystallization
15、CryoPro
Water soluble cryoprotectant reagent set
16、Heavy Atom Screens
Heavy atom kits for multiple isomorphous replacement
17、I3C Phasing Kit
Produce heavy atom derivative of biological macromolecules for phasing

Hampton Optimize Reagents

Hampton Optimize Reagents
1、Optimize – Polymers
Crystallization grade polymers for formulating screens or optimization
2、Optimize – Organics (Volatile)
Crystallization grade organics (volatile) for formulating screens or optimization
3、Optimize – Organics (Non-Volatile)
Crystallization grade organics (non-volatile) for formulating screens or optimization
4、Optimize – Salts
Crystallization grade salts for formulating screens or optimization
5、Optimize – Buffers
Crystallization grade buffers for formulating screens or optimization
6、Optimize – Solubilizing Agents (NDSB)
Crystallization grade non detergent sulfobetaines
7、Optimize – Reducing Agent
TCEP Hydrochloride
8、Optimize – Monoolein
LCP – Lipidic Cubic Phase
9、Optimize – Cryoprotectants
For cryoprotection of crystals
10、Optimize – Oils for Crystallization
Microbatch crystallization & oil layering
11、Optimize – Izit Crystal Dye
Izit™ color dye to differentiate protein crystals from salt crystals

Hampton晶体删选试剂盒

Hampton晶体删选试剂
1.Screens 晶体删选试剂盒

产品名称 货号 用途备注
Index Kit HR2-144 用于生物大分子结晶
CrystalScreen Kit HR2-110 用于蛋白质,核酸水溶性小分子结晶
Crystal Screen 2 Kit HR2-112
Pegrx 1 HR2-082 用于生物大分子结晶
Pegrx 2 HR2-084 用于生物大分子结晶
Peg/Ion Screen Kit HR2-126 用于生物大分子结晶
Peg/Ion 2 Screen™ HR2-098 用于生物大分子结晶
Saltrx Kit HR2-108 用于生物大分子结晶
Membfac Kit HR2-114 用于膜蛋白和溶解度有限的样品结晶
Crystal Screen Lite HR2-128 用于蛋白质,缩氨酸,核酸的结晶
Natrix Kit HR2-116 用于核酸和蛋白核酸复合物的结晶
Crystal Screen Cyro Kit HR2-122 用于蛋白质,可溶性缩氨酸,核酸和水溶性小分子的初次低温结晶
Nucleic Acid Mini Screen Kit HR2-118 用于核酸片段的结晶
Low Ionic Strength Screen HR2-120 用于完整单抗,单抗片段以及在低离子浓度下溶解度较低的样品结晶
Silver Bullet & Silver Bullet Bio HR2-078 用于生物大分子的初次和二次结晶
HR2-080
Peg/Tacsimate Ph5.8 Crystallization Reagent HR2-090
Peg/Tacsimate Ph 6.8 Crystallization Reagent HR2-092
Peg/Tacsimate Ph 7.8 Crystallization Reagent HR2-094
Additive Screen™ HR2-428 利用样品间和样品溶剂间相互作用来优化晶体生长和改变样品溶解度
产品名称 货号 用途备注
Detergent Screen 1 HR2-410 改善疏水作用引起的非特异性聚集
Detergent Screen 2 HR2-411
Detergent Screen 3 HR2-412
Detergent Screen HR2-408
Heavy Atom Screen Pt HR2-442 Heavy Atoms For Multiple Isomorphous Replacement
Heavy Atom Screen Au HR2-444
Heavy Atom Screen Hg HR2-446
Heavy Atom Screen M1 HR2-448
Heavy Atom Screen M2 HR2-450
I3c Phasing Kit HR3-133

2.Custom Shop Crystallization Reagents 结晶试剂盒中单独的试剂

  1. Optimize™ Reagents晶体生长条件优化试剂

⑴ Optimize™ – Polymers聚合体
⑵ Optimize™- Organics (Volatile) 挥发性有机物
⑶ Optimize™- Organics (Non-Volatile)非挥发性的有机物
⑷ Optimize™- Salts 盐类
⑸ Optimize™- Buffers缓冲液
⑹ Optimize™- Solubilizing Agents (NDSB) 溶解试剂NDSB
⑺ Optimize™ – Reducing Agent还原剂-TCEP氢氯化物
⑻ Optimize™ – Cryoprotectants
⑼ Optimize™ – Oils用于结晶的油类
⑽ Optimize™ – Silica Hydrogel硅胶
⑾ Optimize™ – LM Agarose低熔点琼脂糖
5.Crystallization Plates, Hardware & Accessories结晶耗材
⑴ 24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种
⑵ 48 Well Crystallization Plates 48孔晶体板
⑶ 72 Well Crystallization Plates72孔晶体板
⑷ 96 Well Crystallization Plates 96孔晶体板等

Hampton坐滴蛋白结晶板 3 Well Crystallization Plate (Swissci)

Hampton坐滴蛋白结晶板     3 Well Crystallization Plate (Swissci)
3 Well Crystallization Plate (Swissci)
Applications:Sitting drop crystallization  应用于坐滴结晶
该坐滴结晶板的特点:Features

Lens effect drop wells for enhanced optics
Drop volume: 50 nl to 5 碌l
Micro-numbering alongside drop volumes
Reservoir volume: 20 to 40 碌l
ANSI/SLAS 1-2004 Standard
Drop well allows easier crystal harvesting
Three drop wells per reservoir
Midi profile or Low Profile for easier storage
UV compatible (UVP)

Hampton坐滴蛋白结晶板3    Well Crystallization Plate (Swissci)
Description
The SWISSCI 3 Lens Crystallization Plate is an ideal product for protein crystallization presented in a 96-well plate format.
该结晶板是用于在96孔板呈现蛋白质结晶的理想产品
The plate was developed in collaboration with very experienced protein crystallographers. It is the result of many years of experience in successful robotic high-throughput crystallization and combines many of the necessary features not earlier available to the crystallographer.
该板协同经验非常丰富的蛋白质晶体学家开发。这是多年的成功的机器人高通量结晶经验的结果,并结合了许多不早提供给晶体学家必要的功能。
The SWISSCI 3 Lens crystallization Plate offers unique properties that make it ideal for both nanoliter crystallization screening and mircoliter optimization alike. Made from optically superior polymer (UVP) and with a new design of the wells, the plate allows easy crystal viewing and retrieval.The Advantages of the 3 Lens Crystallization Plate    优点:Easy crystal retrieval   易结晶
Raised wide wells make the crystal mounting especially easy.Easy viewing
The wells are a wide conical shape and have a lens effect for perfect illumination. The micro-numbering ensures you will never get lost again (visible by microscope). The optically superior polymer is UV transmissible and may be used to differentiate between salt and protein crystals.Better sealing
Wide partition walls between the wells give plenty of area for good sealing with tape. No central bending occurs in this very robust structure. Excellent long term storage – no sample evaporation.Wide range of volumes
Typical volumes are 20-40 µl of reservoir and 50 nl-5 µl drop size. The 288 optical wells offer three times the number of experimental constructions.ANSI/SLAS 1-2004 Standard
The plates are designed to the 96-well ANSI/SLAS 1-2004 standards for all common holders and external numbering (A-H, 1-12)with corner location make the plate easy to use in a robotic sampler. The 3 Lens plate is suitable for centrifugation. The unique 3 drop protein crystallization plate offers a new way of sitting-drop crystallography. The 288 wells are optically perfect designed to observe crystals under a microscope.3 Lens plate features
Easy to fill 96-lens structure – typically 30 microlitres per buffer lens. Standard ANSI/SLAS 1-2004 form for automation. Optically perfect wells with lens effect for a better view with microscope. Microscopic identifier within wells, simplifying the orientation under the microscope. Maximum volume of the buffer reservoir is 40 microlitres. Grown crystals are easy to identify and to remove from lens due to a low-binding polymer. Plate with 3 lenses for each sample, better growing security with triplicates or the ability to use lens two and lens three as mixing stations. Lenses fill without micro-droplets jumping out due to static effects. Wells can be individually sealed with a perfectly flat upper surface – there is a large land area designed to ensure integrity of each lens section. The profile allows for easier storage. Low volume buffer well enables savings on reagents. Triplicates allow for 288 constructs per plate.3 Well Midi Crystallization Plate (Swissci 3W96TUVP)
3 Well Low Profile Crystallization Plate (Swissci 3W96TLP-UVP)
Hampton坐滴蛋白结晶板  3 Well Crystallization Plate (Swissci)
Sitting drop crystallization

CAT NO NAME DESCRIPTION
HR3-123 3 Well Midi Crystallization Plate (Swissci) 10 plate case
HR3-125 3 Well Midi Crystallization Plate (Swissci) 40 plate case
HR3-205 3 Well Low Profile Crystallization Plate (Swissci) 10 plate case
HR3-206 3 Well Low Profile Crystallization Plate (Swissci) 40 plate case

Hampton 水晶透明密封带Crystal Clear Sealing Tape

Hampton 水晶透明密封带Crystal Clear Sealing Tape
Crystal Clear Sealing Tape
3 inch, 1.88 inch, 1.88 inch with dispenser, 0.75 inch with dispenser (left to right)
Crystal Clear Sealing Tape
0.75 inch Crystal Clear Sealing Tape
Applications  应用
Sealing tape used to seal sitting drop crystallization experiments
Description
These optically clear tapes are compatible with protein crystallization reagents. The polypropylene Crystal Clear Sealing Tapes use acrylic solvent based adhesives which are compatible with aqueous crystallization reagents.
Catalog number HR3-511 is a 1.88 inch (48 mm) wide, 3 mil tape on a 43.7 yard (40 M) roll with a 1.5 inch core and is supplied with a green dispenser / cutter.Catalog number HR4-511 is a 1.88 inch wide, 3 mil tape on a 60 yard roll with a 3 inch core and no dispenser / cutter.Two strips of the HR3-511 or HR4-511 will seal a Corning, Cryschem, CrystalClear Strip, Greiner, Intelliplate, Linbro, Swissci (MRC), VDX and VDXm plate.Catalog number HR4-506 is a 3 inch wide, 3 mil tape on a 54.86 yard roll with a 3 inch core and no dispenser / cutter.One strip of the HR4-506 will seal a Corning, Cryschem M, CrystalClear Strip, Greiner, Intelliplate, Swissci (MRC) and VDXm plate.Crystal Clear Mini Sealing Tape HR4-508 is a 0.75 inch (19 mm) wide, 3 mil tape on a 650 inch (16.5 M) roll and is supplied with a clear dispenser / cutter. The tape is useful for resealing or patching wells after mounting crystals. The tape is also wide enough to seal a single row of a Cryschem S or Cryschem M plate.See chart for tape and plate compatibility.

Hampton 0.1毫米50号玻璃毛细管

Hampton 0.1毫米50号玻璃毛细管

货号:HR6-104
包装:25 pack

  • 英文名:0.1 mm Glass Number 50 Capillary
  • 品牌:Hampton
Hampton 0.1毫米50号玻璃毛细管

Hampton代理–金畔生物

硼硅玻璃50号毛细管壁厚0.01毫米,全长80毫米+ / – 5毫米。提供外径从0.1毫米到2毫米的毛细管,用于X射线数据收集,晶体密度测量和晶体生长实验。毛细管可以用蜡,环氧树脂等密封材料封闭,防止水分和气体。

应用
X射线数据采集
液-液扩散结晶
凝胶针状结晶
特征
50号硼硅玻璃
壁厚 10微米
描述
硼硅玻璃50号毛细管一端呈漏斗形,另一端封闭,壁厚0.01毫米,全长80毫米+/-5毫米,外径从0.1毫米到2毫米。可嵌入,盛放,储存小分子和生物大分子晶体用于X射线数据收集,晶体密度测量和晶体生长实验。毛细管可以用蜡,环氧树脂和其它密封材料密封,防止水分和气体。
请参照“线性吸收系数µcm-1表“,以决定适合的玻璃/石英玻璃毛细管。本表展示的是X射线数据收集时的辐射吸收量。
0.1mm至2.0mm毛细管开口端的基础尺寸为:3.0+/-0.15mm外径 x 0.13+/-0.10 mm壁厚,直径为在毛细管封闭端40毫米处的测量值。
 

货号

产品说明

公差

HR6-104

Size: 0.1 mm – 25 pack

0.1 mm -0.075 / +0.075 mm

HR6-106

Size: 0.2 mm – 25 pack

0.2 mm -0.050 / +0.050 mm

HR6-108

Size: 0.3 mm – 25 pack

0.3 mm -0.050 / +0.100 mm

HR6-110

Size: 0.4 mm – 25 pack

0.4 mm -0.100 / +0.100 mm

HR6-112

Size: 0.5 mm – 25 pack

0.5 mm -0.100 / +0.100 mm

HR6-114

Size: 0.6 mm – 25 pack

0.6 mm -0.100 / +0.100 mm

HR6-116

Size: 0.7 mm – 25 pack

0.7 mm -0.150 / +0.150 mm

HR6-118

Size: 0.8 mm – 25 pack

0.8 mm -0.150 / +0.150 mm

HR6-120

Size: 0.9 mm – 25 pack

0.9 mm -0.150 / +0.150 mm

HR6-122

Size: 1.0 mm – 25 pack

1.0 mm -0.150 / +0.250 mm

HR6-124

Size: 1.5 mm – 25 pack

1.5 mm -0.250 / +0.250 mm

HR6-126

Size: 2.0 mm – 25 pack

2.0 mm -0.250 / +0.250 mm

Hampton结晶用缓冲盐试剂盒– SaltRx

Hampton结晶用缓冲盐试剂盒– SaltRx
SaltRx 1 • SaltRx 2 • SaltRx HT

Applications
Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules
Features

Salt versus pH matrix crystallization screen
Samples pH 4 to 9
22 unique salts versus salt concentration and pH
Compatible with microbatch, vapor diffusion, liquid & gel diffusion methods
Tube or Deep Well block format
Preformulated, ready to screen
Developed at Hampton Research
All salts and buffers in screen readily available as Optimize™ reagents for reproducing and optimizing crystals
Individual reagents available through the Hampton Research Custom Shop
 Description
SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules. Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH. The selection, concentration, and pH of the salts were determined by data mining the BMCD1 and other crystallization databases, crystallization reports in the literature, and internal crystallization trials performed at Hampton Research. Based on this analysis, up to 35% of protein crystallizations involve salt as the primary crystallization reagent. SaltRx can be used as a primary crystallization screen when salt, ionic strength and pH are desired or suspected as appropriate crystallization variables. It is also useful as a secondary screen when salt only reagents/conditions from screens such as Index™, Crystal Screen™, and Grid Screen™ produce crystals and when further screening for additional salt conditions or optimization is desired. Since this screen does not contain volatile organics, it is compatible with microbatch, vapor diffusion, liquid and gel diffusion crystallization methods. SaltRx may also be used for dialysis crystallization in conjunction with Dialysis Buttons™.The original SaltRx kit (HR2-108) contained 96 unique reagents, 10 ml each. This kit has been discontinued and converted into two 48 reagent based kits, SaltRx 1 and SaltRx 2, available separately, which when used together are identical to the original SaltRx.SaltRx 1 contains 48 unique reagents, 10 ml each. SaltRx 1 contains reagents 1-48 of the original SaltRx 96 reagent kit.Salt Rx 2 contains 48 unique reagents, 10 ml each. SaltRx 2 contains reagent 49-96 of the original SaltRx 96 reagent kit.SaltRx HT contains the 96 SaltRx 1 and SaltRx 2 reagents in a single Deep Well block format.Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
Measured pH range of kit is 4.1 to 9.0 at 25°C
Average measured pH of kit is 6.9 at 25°C
Median measured pH of kit is 7.3 at 25°C
Mode measured pH of kit is 7.4 at 25°C
CAT NO NAME DESCRIPTION
HR2-136 SaltRx HT 1 ml, Deep Well block format
HR2-107 SaltRx 1 10 ml, tube format
HR2-109 SaltRx 2 10 ml, tube format

Hampton蛋白结晶工具及仪器

Hampton蛋白结晶工具及仪器
CryoTong™ – Long
ApplicationsCrystal transfer under cryo temperature
FeaturesArtery clamp style closure30 seconds of cryo temperature180 mm length
Description
The CryoTong is a tool used to manually transfer a crystal mounted on a CrystalCap™ from liquid nitrogen to a magnetic base in a goniometer head positioned in a cryogenic stream, and then back to liquid nitrogen. The one-piece, compact CryoTong is available in two lengths. The long CryoTong is approximately 180 mm in overall length. The artery clamp style maintains the CryoTong in the closed position until the clamp is squeezed, which opens the opposing heads. The heads are non-magnetic stainless steel. The handle is magnetic stainless steel. The inside of the head is machined to closely surround the CrystalCap with loop and crystal in place. A small retaining lip is machined into the lower portion of the head to prevent the CrystalCap from slipping out of the CryoTong when the tool is in the closed position. The CryoTong can maintain the temperature of the crystal at -160°C for up to 30 seconds during room temperature crystal transfers. Choose the proper CryoTong size based upon the pin length required by the configuration of the x-ray data collection hardware used. The 18 mm CryoTong (HR5-112) is to be used with the CrystalCap HT and any other CrystalCap configured for 18 mm.HR5-114 Long CryoTong 18 mm, 180° features the head in alignment with the handle of the CryoTong.

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Vial Clamp™ – Straight
ApplicationsVial support and manipulation
FeaturesStraight end tipHemostat style closureDescription
The Vial Clamp™ – Straight is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems™ straight when the clamp is closed. The clamp can be locked using the hemostat style closure. This clamp makes it easy to dip the storage vial into liquid nitrogen for crystal storage. The complete length of the clamp is straight, with an overall length of 195 mm. When the vial is placed in the clamp, the length of it is positioned perpendicular, 90° to the length of the clamp.
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CrystalWand™ Magnetic
ApplicationsCrystalCap Magnetic & CrystalCap Copper Magnetic handling toolDescription
The CrystalWand Magnetic is designed to be used exclusively with the CrystalCap Magnetic, CrystalCap Copper Magnetic, and CrystalCap HT systems during transfer of the caps from the vial to the goniometer head and from the goniometer to the vial. The 6 1/2” (165 mm) long chrome plated steel wand features a plastic housing enclosing a spring tensioned plunger that when depressed, moves a non-magnetic steel platform away from the magnet housed in the end of the wand. This causes the steel CrystalCap to detach from the wand and be placed readily into the vial or the CryoTong™. When the platform is retracted, the wand securely holds the steel CrystalCap Magnetic. The CrystalWand Magnetic 45° (McMiken Tool) offers the same functionality but is bent at a 45° angle at approximately 2.5 cm from the magnet end.HR4-729 CrystalWand Magnetic, StraightHR4-315 CrystalWand Magnetic, 45° (McMiken Tool)
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Vial Clamp™ – Curved
ApplicationsVial support & manipulation
FeaturesCurved end tipHemostat style closureDescription
The Vial Clamp – Curved is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems at an angle when the clamp is closed. The clamp can be locked using the hemostat style closure. The end of the clamp, where the vial is held, is curved at either a 45°/135° or 110°/ 70° angle. When the vial is placed in the clamp, the length of it is positioned to the clamp at either of those same angles. The overall length of each clamp is the same, 195 mm.HR4-671 Vial Clamp – Curved 45°/135°HR4-672 Vial Clamp – Curved 110°/70°
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CrystalCap™ Holder
pplicationsCrystalCap support inside stainless steel dewars
FeaturesAdjustable heightSupports up to 8 CrystalCaps15 ml vial position for liquid propane500 and 1,000 ml versionsDescription
CrystalCap Holders are convenient stands for holding all CrystalCap™ systems in liquid nitrogen dewars during crystal cryogenic procedures. Available in two sizes. Both holders feature spring-clips to secure the CrystalCap Vials in the holder. Both stands have 3 inches of adjustable Z (height) which allows one to raise or lower the CrystalCaps to the appropriate height, depending upon the liquid nitrogen level in the dewar. Each holder is supplied with a socket driver for height adjustment.500 ml version holds five CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 500 ml dewar. 1,000 ml version holds eight CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 1,000 ml dewar.HR4-707 CrystalCap Holder – 500 ml
HR4-706  Replacement Height Adjustment Tool
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Hampton Solubility & Stability Screen

Hampton Solubility & Stability Screen

Solubility & Stability Screen(溶解度和稳定性的屏幕)
应用:溶解性和稳定性屏幕的设计,以协助识别解决方案的条件,促进蛋白质的溶解性和稳定性,并尽量减少蛋白质沉淀。
Solubility & Stability Screen
Solubility & Stability Screen
Solubility & Stability Screen
Solubility & Stability Screen HR2-072
Applications

A solubility screen, a stability screen, an additive screen for protein assays including ThermoFluor and crystallization
Preformulation screening

 

Compatible with Thermofluor , Differential Scanning Fluorimetry (DSF), and Protein Thermal Shift assays
96 sterile filtered reagents
17 classes of reagents and excipients
Highly concentrated (10x) reagent formulation
Developed at Hampton Research

Features
Methodologies

Thermal shift assay
Dynamic Light Scattering
Use Solubility & Stability Screen along side Slice pH and Solubility & Stability Screen 2 to optimize sample buffer
Description

Solubility & Stability Screen is designed to assist in the identification of solution conditions which promote protein solubility and stability, and minimize protein precipitation. Solubility & Stability Screen is a solubility screen, a stability screen, and may also be used as an additive screen in the presence of a crystallization reagent.
The Hampton Research Solubility and Stability Screen can evaluate protein solubility, stability and crystallization in the presence of 94 different chemical additives sampling 17 different classes of reagents plus two controls.
Protein solubility and stability are universally required in a wide range of applications, including general biochemical studies, the preparation of proteins in pharmaceuticals, structural biology and crystallization.1-11The preparation of a concentrated, soluble and stable protein sample can often be a difficult task as proteins often aggregate, precipitate or denature.
Thermofluor (also known as Differential Scanning Fluorimetry, DSF and Protein Thermal Shift) provides a rapid, convenient and effective means of identifying subsaturating solution conditions that may also correlate with protein heterogeneity and poor crystallization outcomes.12
Protein solubility and stability is affected by many different chemical factors including pH, buffer type, chemical additives and excipients. pH and buffer type are dominant protein solubility and stability variables and can be evaluated and optimized using the Hampton Research Slice pH kit HR2-070. Slice pH evaluates protein solubility, stability and crystallization versus 20 different buffers over the pH range 3.5-9.6. Chemical additives influencing protein solubility and stability can be evaluated using the Solubility & Stability Screen.
It is widely accepted that protein solubility and stability can be increased by the use of chemical additives.5,15Seventeen classes of reagents are sampled by the Solubility & Stability Screen and each of these classes has been reported as important in improving sample solubility and stability.2-11
The Solubility & Stability Screen is a set of 94 high purity reagents formulated in high purity water (NCCLS/ASTM Type 1+) at 25°C and are 0.22 micron sterile filtered. The 94 solubility and stability reagents are formulated at 2 to 10 times their recommended working concentration. The remaining two reagents are water and a negative (TCA) control. A water control demonstrates the effect of diluting sample as well as sample buffer concentration. TCA, the negative control, demonstrates total sample precipitation, loss of sample solubility and loss of sample stability. The effects of the Solubility & Stability reagents can be compared with this negative control to assist in the identification of reagents promoting sample solubility and stability. 500 microliters of each reagent is supplied in a sterile 96 well polypropylene Deep Well block. The Solubility & Stability Screen reagents are compatible with the sitting or hanging drop vapor diffusion, microbatch, free interface diffusion, sandwich drop vapor diffusion, and dialysis crystallization methods utilizing water soluble reagents.

Hampton AlumaSeal™ II Sealing Film and Applicator

Hampton AlumaSeal™ II Sealing Film and Applicator

Applications
Sealing film used to reseal HT format screen kits in polypropylene blocks and plates
Features
Excellent seal
Film conforms to raised chimney wells
Easily pierceable with single or multichannel pipettors and robotic probes
Heat & cold resistant, recommended for temperatures from -80 °C to +120 °C
Certified DNase-, RNase-, and nucleic-acid-free
Less evaporation than clear films
Excellent barrier properties, virtually no reagent evaporation or drying
 Description
A 38 µm soft non-permeable aluminum foil sealing film with strong medical-grade adhesive, AlumaSeal II sealing films eliminate the need for heat-sealing devices or mats during the resealing of reagents in polypropylene deep well blocks. Each sealing film measures 82.6 x 142.9 mm and offers sufficient sealing area for 96 deep well blocks. Length between the perforations with end tabs removed is 125.4 mm. Compared to other aluminum foils, AlumaSeal II has less tendency to roll back on itself when removing the backing paper and it conforms well to the plate during application.AlumaSeal II is a soft, pierceable adhesive film designed for the convenient and rapid sealing of polypropylene deep well blocks. A multiple split backing with two end tabs allows for easy, accurate positioning and secure sealing. The use of an adhesive sealing film minimizes evaporation and helps to prevent well-to-well cross contamination in reagent blocks. AlumaSeal II films are easily pierced by pipettte tips or robotic probes or piercing tools for direct reagent recovery without significant gumming by adhesive.
AlumaSeal™ II Sealing Film and Applicator
AlumaSeal™ II Sealing Film and Applicator

CAT NO NAME DESCRIPTION
HR8-069 AlumaSeal II Sealing Film 100 pack
HR4-413 Film Sealing Paddle 5 pack